Q7M6Y6 · MRO2B_MOUSE

  • Protein
    Maestro heat-like repeat-containing protein family member 2B
  • Gene
    Mroh2b
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

May play a role in the process of sperm capacitation (PubMed:27105888).

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentacrosomal vesicle
Cellular Componentcytoplasm
Cellular Componentsperm flagellum
Cellular Componentsperm midpiece
Biological Processcell differentiation
Biological Processprotein kinase A signaling
Biological Processspermatogenesis

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Maestro heat-like repeat-containing protein family member 2B
  • Alternative names
    • HEAT repeat-containing protein 7B2
    • Sperm PKA-interacting factor
      (SPIF
      )

Gene names

    • Name
      Mroh2b
    • Synonyms
      Heatr7b2

Organism names

  • Taxonomic identifier
  • Strain
    • C57BL/6J
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus

Accessions

  • Primary accession
    Q7M6Y6
  • Secondary accessions
    • A0A096XFP7
    • A0A096XFP8

Proteomes

Organism-specific databases

Subcellular Location

Cytoplasm
Note: Colocalizes with PRKACA and TCP11 on the acrosome and tail regions in round spermatids and spermatozoa regardless of the capacitation status of the sperm.

Keywords

Phenotypes & Variants

Disruption phenotype

Embryonically lethal before 10 dpc.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 84 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00003951051-1581Maestro heat-like repeat-containing protein family member 2B

Post-translational modification

Constitutively phosphorylated on serine and threonine residues in acrosomal region of the sperm head, midpiece and flagellar regions of noncapacitated spermatozoa. Phosphorylation on tyrosine residues increases upon sperm capacitation within the acrosomal and tail regions in a protein kinase A (PKA)-dependent signaling pathway.

Proteomic databases

PTM databases

Expression

Tissue specificity

Expressed strongly in round spermatids and fully mature spermatozoa. Expressed weakly in pachytene spermatocytes (at protein level). Isoform 2 is specifically expressed in the testis. Isoform 2 is expressed in pachytene spermatocytes and round spermatids. Isoform 3 is weakly expressed in testis.

Gene expression databases

Interaction

Subunit

Found in a complex at least composed of MROH2B isoform 2, PRKACA isoform 2 and TCP11 (PubMed:27105888).
Interacts with PRKACA isoform 2 (PubMed:27105888).
Interacts with TCP11 (PubMed:27105888).

Protein-protein interaction databases

Miscellaneous

Family & Domains

Features

Showing features for repeat.

TypeIDPosition(s)Description
Repeat123-160HEAT 1
Repeat305-342HEAT 2
Repeat401-441HEAT 3
Repeat464-501HEAT 4
Repeat526-543HEAT 5
Repeat544-580HEAT 6
Repeat658-695HEAT 7
Repeat773-815HEAT 8
Repeat960-997HEAT 9
Repeat1017-1055HEAT 10
Repeat1112-1150HEAT 11
Repeat1153-1191HEAT 12
Repeat1254-1291HEAT 13
Repeat1295-1332HEAT 14
Repeat1359-1379HEAT 15
Repeat1380-1416HEAT 16

Keywords

Phylogenomic databases

Family and domain databases

Sequence & Isoforms

Align isoforms (3)
  • Sequence status
    Complete

This entry describes 3 isoforms produced by Alternative splicing.

Q7M6Y6-1

This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

  • Length
    1,581
  • Mass (Da)
    180,480
  • Last updated
    2010-06-15 v2
  • Checksum
    0314A4B1659ACF95
MEEYGDMFGDINLTIGMLSKEDNISKEDIYCHLTSFIQNTDIMDDAIVQRLIYYTSKDMRDEEIPRELRMLAGEVLVSLAAHDFNSVMYEVQSNFRILELPNEFVVLALAELATSYVSQSIPFMMMTLLTMQTMLRLVEDENMRQTFCIALENFSKSIYKYVNHWKDFPYPKLDANRLSDKIFMLFRYIMEKWAPQASPMHALAIIKAHGPTVSLLLHREDFCEFALSQISWLLLQYRDKENDFYITQSLKQILTAAVLYDIALPKNLRRSVLSSLLHQICKVPEPPIKENKLEASSCFLILAHANPVDLLDFFDEQIRSTNEAVRTGILTLLRSTINAEEPKFRNHTTSIEKTVKLVMGDLSVKVRKSTLLLIQTMCEKGYIEAREGWPLIDYIFSQFAMSNRNLENPIKSNSQEDENGEKSVQETSLEVLKSLDPLVIGMPQVLWPRILTYVVPKEYTGTLDYLFNIIRILIMAEEKKKRDIQESTALVVSTGAVKLPSPQQLLARLLVISILASLGQLCGAGAIGLLKIMPEIIHPKLAEMWKTRMPALLQPLEGSNASIVLWETMLLQLLKESLWKISDVAWTSQLSRDFSLQMGSYSNSSMEKKFLWKALGTTLASCQDKDFVSSQINEFLVTPSLLGDHRQGTTSILGFCAENHLDIVLNVLKTFQDKEKFFVNRCKGIFSGKKSLTKTDLILIYGAVALHAPKQQLLARLDQDIMGQILLLYGQCCQILGVSVINKDMDLQMSFTRSITEVGIAVQDAEDQKFQFTYKEMLIGSMLDLIKDEPLNTLASPVRWKVLIAIRYLSKLKPALSLNDHLNILEENIRRLLPLPPLEKLKSQGETDKDRERIEFLYERSMDALGKLLRSMIWDNTDAQNCEEMFNLLRMWLVSQKQWERERAFQVTSKVLTKDVEAPQNFRIGSLLGLLAPHSCDTLPTIRQAATSSTIGLLCAKGICQEVDRLQGLQEGLDSEDEQVQIKISSKIAKIVCKFIPSEEIQVFLEETLDGLETLDPLCTKACGIWMIAALKEHGALLEDQLLEILSTIYHHMPVLRQKEESFQFMLEAISQIASFHMDAVVNNLLQKPLPFDRDTKTLWKALAENPASSGKLMRALIKKLVARLEDDIAGTEAISVACAIYEVILTGAHITHLYPELFTLLLKLVSCSLGQKMPMSTLSQRRRVMQLGERQRFPDPCRLSTATLKCLQAQAMREGLAKESDEGDNLWTLLSNPDTHHIGVCALARSMAVWQHGVILDIMEHLLSSLTSSSENYRITGMAFFSELMKEPILWKHGNLRDVLIFMDQNARDSNAILRQMAIRGLGNTACGAPHKVRKYKQMMLECIIRGLYHLARTEVVCESLKALKKILELLTERDINFYFKEIVLQTRTFFEDEQDDVRLTAISLFEDLATLTGRRWKIFFAEEVKKSMISFLLHLWDPNPKIGAACRDVLVICIPFLGLQELYGLLDHLLERDLPRARDFYRQLCMKLSKKNQEILWILHTHSFTFFTSSWEMIRSAAVKLTDAIILHLTKRYVELLDREQLTMRLQALRQDPCISVQRAAEATLQTLLRRCKEISIPL

Q7M6Y6-2

  • Name
    2
  • Synonyms
    SPIF-L
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical

Q7M6Y6-3

  • Name
    3
  • Synonyms
    SPIF-S
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical
    • 1-743: MEEYGDMFGDINLTIGMLSKEDNISKEDIYCHLTSFIQNTDIMDDAIVQRLIYYTSKDMRDEEIPRELRMLAGEVLVSLAAHDFNSVMYEVQSNFRILELPNEFVVLALAELATSYVSQSIPFMMMTLLTMQTMLRLVEDENMRQTFCIALENFSKSIYKYVNHWKDFPYPKLDANRLSDKIFMLFRYIMEKWAPQASPMHALAIIKAHGPTVSLLLHREDFCEFALSQISWLLLQYRDKENDFYITQSLKQILTAAVLYDIALPKNLRRSVLSSLLHQICKVPEPPIKENKLEASSCFLILAHANPVDLLDFFDEQIRSTNEAVRTGILTLLRSTINAEEPKFRNHTTSIEKTVKLVMGDLSVKVRKSTLLLIQTMCEKGYIEAREGWPLIDYIFSQFAMSNRNLENPIKSNSQEDENGEKSVQETSLEVLKSLDPLVIGMPQVLWPRILTYVVPKEYTGTLDYLFNIIRILIMAEEKKKRDIQESTALVVSTGAVKLPSPQQLLARLLVISILASLGQLCGAGAIGLLKIMPEIIHPKLAEMWKTRMPALLQPLEGSNASIVLWETMLLQLLKESLWKISDVAWTSQLSRDFSLQMGSYSNSSMEKKFLWKALGTTLASCQDKDFVSSQINEFLVTPSLLGDHRQGTTSILGFCAENHLDIVLNVLKTFQDKEKFFVNRCKGIFSGKKSLTKTDLILIYGAVALHAPKQQLLARLDQDIMGQILLLYGQCCQILGVSVINK → MAHAKSIPNASVPQEQIQTLQHKLDYGSDCSFHFIALPTIYSGNIITFLPAKCRAFFEPKNPSLPFLLLLFVFYLP

Sequence caution

The sequence DAA01465.1 differs from that shown. Reason: Erroneous initiation Truncated N-terminus.

Features

Showing features for alternative sequence, sequence conflict.

TypeIDPosition(s)Description
Alternative sequenceVSP_0587211-743in isoform 3
Sequence conflict401in Ref. 1; AHK25002
Sequence conflict659in Ref. 1; AHK25002
Alternative sequenceVSP_058722739-743in isoform 2

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
KF778378
EMBL· GenBank· DDBJ
AHK25002.1
EMBL· GenBank· DDBJ
mRNA
KF778379
EMBL· GenBank· DDBJ
AHK25003.1
EMBL· GenBank· DDBJ
mRNA
AC115877
EMBL· GenBank· DDBJ
-Genomic DNA No translation available.
AC117588
EMBL· GenBank· DDBJ
-Genomic DNA No translation available.
BK001331
EMBL· GenBank· DDBJ
DAA01465.1
EMBL· GenBank· DDBJ
mRNA Different initiation

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
FeedbackHelp