Q7LXU0 · HJC_SACS2
- ProteinCrossover junction endodeoxyribonuclease Hjc
- Genehjc
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids143 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
A structure-specific endonuclease that resolves Holliday junction (HJ) intermediates during genetic recombination; may have some degree of sequence preference in a mobile junction. Cleaves 4-way DNA junctions introducing paired nicks in opposing strands, leaving a 5'-terminal phosphate and a 3'-terminal hydroxyl group that are subsequently ligated to produce recombinant products. Can cleave all 4 strands 3 bases 3' of the junction center. Cleaves both mobile and immobile junctions. Modifies the structure of the 4-way DNA junction, a model Holliday junction structure. The protein forms multiple complexes with 4-way DNA, suggesting more than 1 homodimer can bind to each junction.
Miscellaneous
A second Holliday junction resolving enzyme, Hje, with different substrate specificity exists in this organism.
Catalytic activity
Cofactor
Note: Binds 1 Mg2+ ion per subunit.
Activity regulation
Autoinhibits at very high concentrations, possibly because of extreme junction distortion. Inhibition (and activity at low concentrations of enzyme) is stimulated by dsDNA and Sso7d (PubMed:11709558).
Activity stimulated by PCNA subunit PCNA1 (PubMed:17011573).
Activity stimulated by PCNA subunit PCNA1 (PubMed:17011573).
Features
Showing features for binding site, active site, site.
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | crossover junction DNA endonuclease activity | |
Molecular Function | DNA binding | |
Molecular Function | magnesium ion binding | |
Biological Process | DNA recombination | |
Biological Process | DNA repair |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameCrossover junction endodeoxyribonuclease Hjc
- EC number
- Short namesHjc
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageArchaea > Thermoproteota > Thermoprotei > Sulfolobales > Sulfolobaceae > Saccharolobus
Accessions
- Primary accessionQ7LXU0
- Secondary accessions
Proteomes
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 12 | No cleavage of 4-way junction DNA. Binds junction DNA normally. | ||||
Sequence: E → Q | ||||||
Mutagenesis | 32 | No cleavage of fixed 4-way junction DNA. Binds junction DNA normally. | ||||
Sequence: S → A | ||||||
Mutagenesis | 42 | No cleavage of 4-way junction DNA. Binds junction DNA normally. | ||||
Sequence: D → N | ||||||
Mutagenesis | 55 | No cleavage of 4-way junction DNA. Binds junction DNA normally. | ||||
Sequence: E → Q | ||||||
Mutagenesis | 57 | 1000-fold less cleavage of 4-way junction DNA. Binds junction DNA normally. | ||||
Sequence: K → A | ||||||
Mutagenesis | 137-143 | No interaction with PCNA1. | ||||
Sequence: Missing |
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000429158 | 1-143 | Crossover junction endodeoxyribonuclease Hjc | |||
Sequence: MNAKKRKGSAVERNIVSRLRDKGFAVVRAPASGSKRKDPIPDIIALKNGVIILIEMKSRKDIEGKIYVRREQAEGIIEFARKSGGSLFLGVKKPGVLKFIPFEKLRRTETGNYVADSEIEGLDLEDLVRLVEAKISRTLDNFL |
Proteomic databases
Interaction
Subunit
Homodimer (Probable). Interacts with PCNA subunit PCNA1.
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length143
- Mass (Da)16,010
- Last updated2011-05-31 v1
- Checksum22099074AECF1279
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
Y18930 EMBL· GenBank· DDBJ | CAB57725.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AE006641 EMBL· GenBank· DDBJ | AAK40889.1 EMBL· GenBank· DDBJ | Genomic DNA |