Q7K5M0 · SCARF_DROME

Function

function

Inactive serine protease that plays a role in germ-band retraction and dorsal closure morphogenesis in embryogenesis; contributes to amnioserosa attachment and epithelial apico-basal polarity by regulating the localization of laminin LanA on the apical side of the amnioserosa epithelium (PubMed:20379222, PubMed:28628612).
Contributes to epithelial morphogenesis probably by regulating the bsk/JNK pathway, as part of a negative-feedback loop, and by modulating the cross-talk between the Egfr, bsk/JNK and dpp signal transduction pathways (PubMed:20379222, PubMed:28628612).
In larval development, antagonizes the morphogenetic movements controlled by the bsk/JNK signaling including male genitalia formation and thorax development (PubMed:20379222, PubMed:20530545, PubMed:25737837).

Caution

Lacks the conserved residues within the catalytic triad, probably resulting in a loss of proteolytic activity.

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentcytoplasm
Cellular Componentextracellular region
Cellular Componentextracellular space
Biological Processdorsal closure
Biological Processdorsal closure, elongation of leading edge cells
Biological Processestablishment or maintenance of epithelial cell apical/basal polarity
Biological Processgerm-band shortening
Biological Processimaginal disc morphogenesis
Biological Processimaginal disc-derived male genitalia morphogenesis
Biological Processnotum morphogenesis
Biological Processproteolysis
Biological Processregulation of developmental process

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Inactive serine protease scarface

Gene names

    • Name
      scaf
    • Synonyms
      scarf
    • ORF names
      CG11066

Organism names

  • Taxonomic identifier
  • Strain
    • Berkeley
  • Taxonomic lineage
    Eukaryota > Metazoa > Ecdysozoa > Arthropoda > Hexapoda > Insecta > Pterygota > Neoptera > Endopterygota > Diptera > Brachycera > Muscomorpha > Ephydroidea > Drosophilidae > Drosophila > Sophophora

Accessions

  • Primary accession
    Q7K5M0

Proteomes

Organism-specific databases

Subcellular Location

Keywords

Phenotypes & Variants

Disruption phenotype

Embryonic lethal due to defects in the dorsal closure and germ-band retraction; these defects include undifferentiated or defective larval cuticles presenting dorsal holes and wrinkles, defective attachment of the amnioserosa to the tail end of the germ band, defective elongation of the lateral ectoderm with compromised interface between the LE cells and the amnioserosa (PubMed:15342518, PubMed:20379222, PubMed:20530545).
RNAi-mediated knockdown results in pupal lethality and scarring (PubMed:25737837).
RNAi-mediated knockdown in the epidermis results in loss of wing veins and thorax mechanosensory bristles as well as male terminalia malformations including genitalia rotation defects and partial dissociation of the genital plate from the abdomen (PubMed:20530545).
RNAi-mediated knockdown in the dorsal compartment of the wing disk results in loss of bristles from the medio-lateral region of the thorax and in the appearance of a mild thoracic cleft (PubMed:25737837).
RNAi-mediated knockdown in the notum area of the wing disk, destined to form the dorsal medio-lateral region of the adult thorax as well as thoracic bristles, results in a thoracic cleft and loss of bristles from the medio-lateral region of the thorax (PubMed:25737837).

PTM/Processing

Features

Showing features for signal, chain, disulfide bond.

TypeIDPosition(s)Description
Signal1-24
ChainPRO_501068360025-655Inactive serine protease scarface
Disulfide bond344↔394
Disulfide bond350↔383
Disulfide bond356↔395
Disulfide bond450↔466
Disulfide bond547↔605
Disulfide bond579↔587
Disulfide bond595↔623

Keywords

Proteomic databases

Expression

Developmental stage

In embryos, expressed in leading edge (LE) cells (at protein level) (PubMed:20530545).
In larvae, expressed in the wing imaginal disk cells in the future hinge region, specifically in the peripodial stalk and in the peripodial membrane cells (at protein level) (PubMed:15342518, PubMed:25737837).
In larvae, expressed in the A8 abdomen-derived cells in the male genital disk (at protein level) (PubMed:20530545).
In third instar larvae, expressed in the anterior and posterios termini (at protein level) (PubMed:15342518).
In adult fly, expressed in the wing hinge, in the socket cells of the micro- and macrochaete and proboscis (at protein level) (PubMed:15342518).
In embryos, expressed in leading edge (LE) cells during germ-band retraction and dorsal closure from stage 13; expressed in some cells of the amnioserosa in particular in the posterior canthus as well as in the ventral ectoderm; expressed both in head and tail region (PubMed:20379222, PubMed:20530545, PubMed:28628612).

Gene expression databases

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for region, compositional bias, domain.

TypeIDPosition(s)Description
Region213-319Disordered
Compositional bias215-248Polar residues
Region343-407CLIP
Domain421-644Peptidase S1

Domain

The CLIP domain consists of 37-55 residues which are 'knitted' together usually by 3 conserved disulfide bonds forming a clip-like compact structure.

Sequence similarities

Belongs to the peptidase S1 family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    655
  • Mass (Da)
    71,417
  • Last updated
    2006-10-03 v1
  • Checksum
    4726435D771ABF14
MSASHFREQLALCITLAVLAAASGDYRANMFLNGQYQNGIKDQKENNLLVNPSTNVFLNHAIISRQASPFQGPTYLPPKEFLKCAPGQQCVRSGQCLNGYFAQQLPKIQNCDPETTVCCTYRPPPTTTTTTTTSVPVANCAYDSDCVTPDNCRNGEISAINYVKKQGPNRCPAPNICCRIPSTTLTEDGYIFNLPEKTFPLPTKPAVLAMPSTQAPFRPQPTTAVPASRPTIEYLPPSTTQHPSYEKVQTSRRPVYLPPSPATESASSLIPKIRPRPEPRPQPTRRPTNEYLPPAAANEIPRFEPDRAPQPSNQKPIYRGEDQLSPQIFPTPQPANVPKHFAKCASALVCTSENFCNAIGVLSETPVELSPMEAAFRVPLTDCLQTENGSPGKCCRDPNYVDPWPVNLAGVCATRNKRTKPTGVKDLDANFAEIPWQAMILRESSKTLICGGAIIGDQFVLSSASCVNGLPVTDIRVKAGEWELGSTNEPLPFQLTGVKTVDVHPDYDPSTNSHDLAIIRLERRLEFASHIQPICISDEDPKDSEQCFTSGWGKQALSIHEEGALMHVTDTLPQARSECSADSSSVCSATKFDSCQFDVGSALACGSGSSVRLKGIFAGENSCGEGQTVRFAKPDIKWINTAFAENNKPLLLKRF

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias215-248Polar residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AE013599
EMBL· GenBank· DDBJ
AAF57320.2
EMBL· GenBank· DDBJ
Genomic DNA
AE013599
EMBL· GenBank· DDBJ
AAM68354.1
EMBL· GenBank· DDBJ
Genomic DNA
AY047548
EMBL· GenBank· DDBJ
AAK77280.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

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