Q7K5M0 · SCARF_DROME
- ProteinInactive serine protease scarface
- Genescaf
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids655 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Inactive serine protease that plays a role in germ-band retraction and dorsal closure morphogenesis in embryogenesis; contributes to amnioserosa attachment and epithelial apico-basal polarity by regulating the localization of laminin LanA on the apical side of the amnioserosa epithelium (PubMed:20379222, PubMed:28628612).
Contributes to epithelial morphogenesis probably by regulating the bsk/JNK pathway, as part of a negative-feedback loop, and by modulating the cross-talk between the Egfr, bsk/JNK and dpp signal transduction pathways (PubMed:20379222, PubMed:28628612).
In larval development, antagonizes the morphogenetic movements controlled by the bsk/JNK signaling including male genitalia formation and thorax development (PubMed:20379222, PubMed:20530545, PubMed:25737837).
Contributes to epithelial morphogenesis probably by regulating the bsk/JNK pathway, as part of a negative-feedback loop, and by modulating the cross-talk between the Egfr, bsk/JNK and dpp signal transduction pathways (PubMed:20379222, PubMed:28628612).
In larval development, antagonizes the morphogenetic movements controlled by the bsk/JNK signaling including male genitalia formation and thorax development (PubMed:20379222, PubMed:20530545, PubMed:25737837).
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | extracellular region | |
Cellular Component | extracellular space | |
Biological Process | dorsal closure | |
Biological Process | dorsal closure, elongation of leading edge cells | |
Biological Process | establishment or maintenance of epithelial cell apical/basal polarity | |
Biological Process | germ-band shortening | |
Biological Process | imaginal disc morphogenesis | |
Biological Process | imaginal disc-derived male genitalia morphogenesis | |
Biological Process | notum morphogenesis | |
Biological Process | proteolysis | |
Biological Process | regulation of developmental process |
Keywords
- Molecular function
Names & Taxonomy
Protein names
- Recommended nameInactive serine protease scarface
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Metazoa > Ecdysozoa > Arthropoda > Hexapoda > Insecta > Pterygota > Neoptera > Endopterygota > Diptera > Brachycera > Muscomorpha > Ephydroidea > Drosophilidae > Drosophila > Sophophora
Accessions
- Primary accessionQ7K5M0
Proteomes
Organism-specific databases
Subcellular Location
Phenotypes & Variants
Disruption phenotype
Embryonic lethal due to defects in the dorsal closure and germ-band retraction; these defects include undifferentiated or defective larval cuticles presenting dorsal holes and wrinkles, defective attachment of the amnioserosa to the tail end of the germ band, defective elongation of the lateral ectoderm with compromised interface between the LE cells and the amnioserosa (PubMed:15342518, PubMed:20379222, PubMed:20530545).
RNAi-mediated knockdown results in pupal lethality and scarring (PubMed:25737837).
RNAi-mediated knockdown in the epidermis results in loss of wing veins and thorax mechanosensory bristles as well as male terminalia malformations including genitalia rotation defects and partial dissociation of the genital plate from the abdomen (PubMed:20530545).
RNAi-mediated knockdown in the dorsal compartment of the wing disk results in loss of bristles from the medio-lateral region of the thorax and in the appearance of a mild thoracic cleft (PubMed:25737837).
RNAi-mediated knockdown in the notum area of the wing disk, destined to form the dorsal medio-lateral region of the adult thorax as well as thoracic bristles, results in a thoracic cleft and loss of bristles from the medio-lateral region of the thorax (PubMed:25737837).
RNAi-mediated knockdown results in pupal lethality and scarring (PubMed:25737837).
RNAi-mediated knockdown in the epidermis results in loss of wing veins and thorax mechanosensory bristles as well as male terminalia malformations including genitalia rotation defects and partial dissociation of the genital plate from the abdomen (PubMed:20530545).
RNAi-mediated knockdown in the dorsal compartment of the wing disk results in loss of bristles from the medio-lateral region of the thorax and in the appearance of a mild thoracic cleft (PubMed:25737837).
RNAi-mediated knockdown in the notum area of the wing disk, destined to form the dorsal medio-lateral region of the adult thorax as well as thoracic bristles, results in a thoracic cleft and loss of bristles from the medio-lateral region of the thorax (PubMed:25737837).
PTM/Processing
Features
Showing features for signal, chain, disulfide bond.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Signal | 1-24 | |||||
Sequence: MSASHFREQLALCITLAVLAAASG | ||||||
Chain | PRO_5010683600 | 25-655 | Inactive serine protease scarface | |||
Sequence: DYRANMFLNGQYQNGIKDQKENNLLVNPSTNVFLNHAIISRQASPFQGPTYLPPKEFLKCAPGQQCVRSGQCLNGYFAQQLPKIQNCDPETTVCCTYRPPPTTTTTTTTSVPVANCAYDSDCVTPDNCRNGEISAINYVKKQGPNRCPAPNICCRIPSTTLTEDGYIFNLPEKTFPLPTKPAVLAMPSTQAPFRPQPTTAVPASRPTIEYLPPSTTQHPSYEKVQTSRRPVYLPPSPATESASSLIPKIRPRPEPRPQPTRRPTNEYLPPAAANEIPRFEPDRAPQPSNQKPIYRGEDQLSPQIFPTPQPANVPKHFAKCASALVCTSENFCNAIGVLSETPVELSPMEAAFRVPLTDCLQTENGSPGKCCRDPNYVDPWPVNLAGVCATRNKRTKPTGVKDLDANFAEIPWQAMILRESSKTLICGGAIIGDQFVLSSASCVNGLPVTDIRVKAGEWELGSTNEPLPFQLTGVKTVDVHPDYDPSTNSHDLAIIRLERRLEFASHIQPICISDEDPKDSEQCFTSGWGKQALSIHEEGALMHVTDTLPQARSECSADSSSVCSATKFDSCQFDVGSALACGSGSSVRLKGIFAGENSCGEGQTVRFAKPDIKWINTAFAENNKPLLLKRF | ||||||
Disulfide bond | 344↔394 | |||||
Sequence: CASALVCTSENFCNAIGVLSETPVELSPMEAAFRVPLTDCLQTENGSPGKC | ||||||
Disulfide bond | 350↔383 | |||||
Sequence: CTSENFCNAIGVLSETPVELSPMEAAFRVPLTDC | ||||||
Disulfide bond | 356↔395 | |||||
Sequence: CNAIGVLSETPVELSPMEAAFRVPLTDCLQTENGSPGKCC | ||||||
Disulfide bond | 450↔466 | |||||
Sequence: CGGAIIGDQFVLSSASC | ||||||
Disulfide bond | 547↔605 | |||||
Sequence: CFTSGWGKQALSIHEEGALMHVTDTLPQARSECSADSSSVCSATKFDSCQFDVGSALAC | ||||||
Disulfide bond | 579↔587 | |||||
Sequence: CSADSSSVC | ||||||
Disulfide bond | 595↔623 | |||||
Sequence: CQFDVGSALACGSGSSVRLKGIFAGENSC |
Keywords
- PTM
Proteomic databases
Expression
Developmental stage
In embryos, expressed in leading edge (LE) cells (at protein level) (PubMed:20530545).
In larvae, expressed in the wing imaginal disk cells in the future hinge region, specifically in the peripodial stalk and in the peripodial membrane cells (at protein level) (PubMed:15342518, PubMed:25737837).
In larvae, expressed in the A8 abdomen-derived cells in the male genital disk (at protein level) (PubMed:20530545).
In third instar larvae, expressed in the anterior and posterios termini (at protein level) (PubMed:15342518).
In adult fly, expressed in the wing hinge, in the socket cells of the micro- and macrochaete and proboscis (at protein level) (PubMed:15342518).
In embryos, expressed in leading edge (LE) cells during germ-band retraction and dorsal closure from stage 13; expressed in some cells of the amnioserosa in particular in the posterior canthus as well as in the ventral ectoderm; expressed both in head and tail region (PubMed:20379222, PubMed:20530545, PubMed:28628612).
In larvae, expressed in the wing imaginal disk cells in the future hinge region, specifically in the peripodial stalk and in the peripodial membrane cells (at protein level) (PubMed:15342518, PubMed:25737837).
In larvae, expressed in the A8 abdomen-derived cells in the male genital disk (at protein level) (PubMed:20530545).
In third instar larvae, expressed in the anterior and posterios termini (at protein level) (PubMed:15342518).
In adult fly, expressed in the wing hinge, in the socket cells of the micro- and macrochaete and proboscis (at protein level) (PubMed:15342518).
In embryos, expressed in leading edge (LE) cells during germ-band retraction and dorsal closure from stage 13; expressed in some cells of the amnioserosa in particular in the posterior canthus as well as in the ventral ectoderm; expressed both in head and tail region (PubMed:20379222, PubMed:20530545, PubMed:28628612).
Gene expression databases
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region, compositional bias, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 213-319 | Disordered | ||||
Sequence: TQAPFRPQPTTAVPASRPTIEYLPPSTTQHPSYEKVQTSRRPVYLPPSPATESASSLIPKIRPRPEPRPQPTRRPTNEYLPPAAANEIPRFEPDRAPQPSNQKPIYR | ||||||
Compositional bias | 215-248 | Polar residues | ||||
Sequence: APFRPQPTTAVPASRPTIEYLPPSTTQHPSYEKV | ||||||
Region | 343-407 | CLIP | ||||
Sequence: KCASALVCTSENFCNAIGVLSETPVELSPMEAAFRVPLTDCLQTENGSPGKCCRDPNYVDPWPVN | ||||||
Domain | 421-644 | Peptidase S1 | ||||
Sequence: PTGVKDLDANFAEIPWQAMILRESSKTLICGGAIIGDQFVLSSASCVNGLPVTDIRVKAGEWELGSTNEPLPFQLTGVKTVDVHPDYDPSTNSHDLAIIRLERRLEFASHIQPICISDEDPKDSEQCFTSGWGKQALSIHEEGALMHVTDTLPQARSECSADSSSVCSATKFDSCQFDVGSALACGSGSSVRLKGIFAGENSCGEGQTVRFAKPDIKWINTAFA |
Domain
The CLIP domain consists of 37-55 residues which are 'knitted' together usually by 3 conserved disulfide bonds forming a clip-like compact structure.
Sequence similarities
Belongs to the peptidase S1 family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length655
- Mass (Da)71,417
- Last updated2006-10-03 v1
- Checksum4726435D771ABF14
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 215-248 | Polar residues | ||||
Sequence: APFRPQPTTAVPASRPTIEYLPPSTTQHPSYEKV |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AE013599 EMBL· GenBank· DDBJ | AAF57320.2 EMBL· GenBank· DDBJ | Genomic DNA | ||
AE013599 EMBL· GenBank· DDBJ | AAM68354.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AY047548 EMBL· GenBank· DDBJ | AAK77280.1 EMBL· GenBank· DDBJ | mRNA |