Q7JYX0 · GSTEE_DROME

Function

function

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles (PubMed:22082028).
Essential for ecdysteroid biosynthesis (PubMed:25300303, PubMed:25344753).
May be involved in detoxification (PubMed:22082028).

Miscellaneous

Member of the Halloween gene group.

Catalytic activity

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
3.84 mMglutathione
0.28 mM1-chloro-2,4-dinitrobenzene
Vmax pH TEMPERATURE[C] NOTES EVIDENCE
133 μmol/min/mgwith 1-chloro-2,4-dinitrobenzene as substrate
2.75 μmol/min/mgwith 4-hydroxy-2-nonenal as substrate
0.322 μmol/min/mgwith phenethyl isothiocyanate as substrate

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular Functionglutathione transferase activity
Molecular Functionsteroid delta-isomerase activity
Biological Processcholesterol homeostasis
Biological Processecdysteroid biosynthetic process
Biological Processglutathione metabolic process
Biological Processresponse to toxic substance

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Glutathione S-transferase E14
  • EC number
  • Alternative names
    • Protein noppera-bo

Gene names

    • Name
      GstE14
    • Synonyms
      GSTD14-14
      , nobo
    • ORF names
      CG4688

Organism names

  • Taxonomic identifier
  • Strain
    • Berkeley
  • Taxonomic lineage
    Eukaryota > Metazoa > Ecdysozoa > Arthropoda > Hexapoda > Insecta > Pterygota > Neoptera > Endopterygota > Diptera > Brachycera > Muscomorpha > Ephydroidea > Drosophilidae > Drosophila > Sophophora

Accessions

  • Primary accession
    Q7JYX0

Proteomes

Organism-specific databases

Subcellular Location

Phenotypes & Variants

Disruption phenotype

Embryonic lethal. Embryos display severe developmental defects such as poorly differentiated cuticle, failure of head involution, abnormal gut looping, and defective mouth hooks and dorsal closure (PubMed:25300303, PubMed:25344753).
Trichromes are absent (PubMed:25344753).
Embryos have a higher sterol content (PubMed:25300303, PubMed:25344753).
Embryonic lethality, defective epidermal differentiation and trichome development can be rescued by supplementing embryos with 20-hydroxyecdysone (20E), ecdysone or cholesterol (PubMed:25344753).
RNAi-mediated knockdown results in larval lethality at the second instar stage, which can be rescued by supplementing larvae with 20E, ecdysone or cholesterol (PubMed:25300303).

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004336441-232Glutathione S-transferase E14

Proteomic databases

Expression

Tissue specificity

Expressed in the adult ovary (at protein level).

Developmental stage

In early embryos expression is ubiquitous (PubMed:25344753).
From mid-embryogenesis (stage 16) and throughout larval development, expressed in the prothoracic gland cells of the ring gland (PubMed:25300303, PubMed:25344753).
Weak expression in the follicle cells of the ovarioles in developing egg chambers (at protein level) (PubMed:25300303).

Gene expression databases

    • FBgn0033817Expressed in head capsule and 12 other cell types or tissues

Interaction

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain4-85GST N-terminal
Domain91-218GST C-terminal

Sequence similarities

Belongs to the GST superfamily. Epsilon family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    232
  • Mass (Da)
    26,622
  • Last updated
    2006-10-03 v1
  • Checksum
    81BDFB41C6FBD87B
MSQPKPILYYDERSPPVRSCLMLIKLLDIDVELRFVNLFKGEQFQKDFLALNPQHSVPTLVHGDLVLTDSHAILIHLAEKFDEGGSLWPQEHAERMKVLNLLLFECSFLFRRDSDFMSATVRQGFANVDVAHHERKLTEAYIIMERYLENSDFMAGPQLTLADLSIVTTLSTVNLMFPLSQFPRLRRWFTAMQQLDAYEANCSGLEKLRQTMESVGSFQFPSSSAVVTEKVE

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AE013599
EMBL· GenBank· DDBJ
AAF58397.1
EMBL· GenBank· DDBJ
Genomic DNA
AY071713
EMBL· GenBank· DDBJ
AAL49335.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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