Q7JYX0 · GSTEE_DROME
- ProteinGlutathione S-transferase E14
- GeneGstE14
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids232 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles (PubMed:22082028).
Essential for ecdysteroid biosynthesis (PubMed:25300303, PubMed:25344753).
May be involved in detoxification (PubMed:22082028).
Essential for ecdysteroid biosynthesis (PubMed:25300303, PubMed:25344753).
May be involved in detoxification (PubMed:22082028).
Miscellaneous
Member of the Halloween gene group.
Catalytic activity
- RX + glutathione = an S-substituted glutathione + a halide anion + H+
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
3.84 mM | glutathione | |||||
0.28 mM | 1-chloro-2,4-dinitrobenzene |
Vmax | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|
133 μmol/min/mg | with 1-chloro-2,4-dinitrobenzene as substrate | ||||
2.75 μmol/min/mg | with 4-hydroxy-2-nonenal as substrate | ||||
0.322 μmol/min/mg | with phenethyl isothiocyanate as substrate |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | glutathione transferase activity | |
Molecular Function | steroid delta-isomerase activity | |
Biological Process | cholesterol homeostasis | |
Biological Process | ecdysteroid biosynthetic process | |
Biological Process | glutathione metabolic process | |
Biological Process | response to toxic substance |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameGlutathione S-transferase E14
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Metazoa > Ecdysozoa > Arthropoda > Hexapoda > Insecta > Pterygota > Neoptera > Endopterygota > Diptera > Brachycera > Muscomorpha > Ephydroidea > Drosophilidae > Drosophila > Sophophora
Accessions
- Primary accessionQ7JYX0
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Phenotypes & Variants
Disruption phenotype
Embryonic lethal. Embryos display severe developmental defects such as poorly differentiated cuticle, failure of head involution, abnormal gut looping, and defective mouth hooks and dorsal closure (PubMed:25300303, PubMed:25344753).
Trichromes are absent (PubMed:25344753).
Embryos have a higher sterol content (PubMed:25300303, PubMed:25344753).
Embryonic lethality, defective epidermal differentiation and trichome development can be rescued by supplementing embryos with 20-hydroxyecdysone (20E), ecdysone or cholesterol (PubMed:25344753).
RNAi-mediated knockdown results in larval lethality at the second instar stage, which can be rescued by supplementing larvae with 20E, ecdysone or cholesterol (PubMed:25300303).
Trichromes are absent (PubMed:25344753).
Embryos have a higher sterol content (PubMed:25300303, PubMed:25344753).
Embryonic lethality, defective epidermal differentiation and trichome development can be rescued by supplementing embryos with 20-hydroxyecdysone (20E), ecdysone or cholesterol (PubMed:25344753).
RNAi-mediated knockdown results in larval lethality at the second instar stage, which can be rescued by supplementing larvae with 20E, ecdysone or cholesterol (PubMed:25300303).
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000433644 | 1-232 | Glutathione S-transferase E14 | ||
Proteomic databases
Expression
Tissue specificity
Expressed in the adult ovary (at protein level).
Developmental stage
In early embryos expression is ubiquitous (PubMed:25344753).
From mid-embryogenesis (stage 16) and throughout larval development, expressed in the prothoracic gland cells of the ring gland (PubMed:25300303, PubMed:25344753).
Weak expression in the follicle cells of the ovarioles in developing egg chambers (at protein level) (PubMed:25300303).
From mid-embryogenesis (stage 16) and throughout larval development, expressed in the prothoracic gland cells of the ring gland (PubMed:25300303, PubMed:25344753).
Weak expression in the follicle cells of the ovarioles in developing egg chambers (at protein level) (PubMed:25300303).
Gene expression databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 4-85 | GST N-terminal | |||
Domain | 91-218 | GST C-terminal | |||
Sequence similarities
Belongs to the GST superfamily. Epsilon family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length232
- Mass (Da)26,622
- Last updated2006-10-03 v1
- Checksum81BDFB41C6FBD87B
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AE013599 EMBL· GenBank· DDBJ | AAF58397.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AY071713 EMBL· GenBank· DDBJ | AAL49335.1 EMBL· GenBank· DDBJ | mRNA |