Q7AP87 · INLH_LISMO
- ProteinInternalin H
- GeneinlH
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids548 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Contributes to systemic listeriosis in mice by decreasing host IL-6 cytokine production and thus evasion of the host immune response. Does not contribute to invasion of the host intestinal tissue.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | extracellular region |
Keywords
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameInternalin H
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Bacillota > Bacilli > Bacillales > Listeriaceae > Listeria
Accessions
- Primary accessionQ7AP87
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Secreted, cell wall ; Peptidoglycan-anchor
Note: In the absence of SrtA in exponential phase some protein is still anchored to the cell wall while protein levels are the same in the srtA mutant; in stationary phase much less protein accumulates (PubMed:22837151).
During exponential growth detected on the cell surface as a series of dots in a helical pattern, it is excluded from the septum (PubMed:21725001).
The helical pattern of InlA does not overlap with that of InlH, InlJ or Hbp2 (SvpA) (PubMed:21725001).
In stationary phase colocalizes with InlA at cell poles and at the septum, the location shift requires SigB (PubMed:21725001).
During exponential growth detected on the cell surface as a series of dots in a helical pattern, it is excluded from the septum (PubMed:21725001).
The helical pattern of InlA does not overlap with that of InlH, InlJ or Hbp2 (SvpA) (PubMed:21725001).
In stationary phase colocalizes with InlA at cell poles and at the septum, the location shift requires SigB (PubMed:21725001).
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
Increased expression of InlA (PubMed:20176794).
No change in invasion or bacterial survival in host mamammalian cells including macrophages (PubMed:20176794).
Decreased bacterial counts in blood, spleen and liver of intravenously inoculated BALB/c and C57BL/6 mice (PubMed:11575932, PubMed:20176794).
Increased production of host IL-6 in liver and spleen of intravenously infected BALB/c mice, but not in macrophage cell lines (PubMed:20176794).
No change in invasion or bacterial survival in host mamammalian cells including macrophages (PubMed:20176794).
Decreased bacterial counts in blood, spleen and liver of intravenously inoculated BALB/c and C57BL/6 mice (PubMed:11575932, PubMed:20176794).
Increased production of host IL-6 in liver and spleen of intravenously infected BALB/c mice, but not in macrophage cell lines (PubMed:20176794).
PTM/Processing
Features
Showing features for signal, chain, modified residue, propeptide.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Signal | 1-30 | |||||
Sequence: MKKRWNSVFKLVLMVTAILGLSLYVTTSQG | ||||||
Chain | PRO_5004286629 | 31-548 | Internalin H | |||
Sequence: VEVRAESITQPTAINVIFPDPALANAIKIAAGKSNVTDTVTQADLDGITTLSAFGTGVTTIEGVQYLNNLIGLELKDNQITDLTPLKNLTKITELELSGNPLKNVSAIAGLQSIKTLDLTSTQITDVTPLAGLSNLQVLYLDLNQITNISPLAGLTNLQYLSIGNAQVSDLTPLANLSKLTTLKADDNKISDISPLASLPNLIEVHLKNNQISDVSPLANTSNLFIVTLTNQTITNQPVFYQNNLVVPNVVKGPSGAPIAPATISDNGTYASPNLTWNLTSFINNVSYTFNQSVTFKNTTVPFSGTVTQPLTEAYTAVFDVDGKQTSVTVGANELIKEPTAPTKEGYTFTGWYDAKTGGTKWDFATDKMPAEDITLYAQFTINSYTATFDIDGKLTTQKVTYQSLLEEPVAPTKDGYTFTGWYDAKTGGTKWDFATGKMPAGNITLYAQFTKNDNPNPDDPTTNTPTGNGDGTSNPSNSGGNTTLPTAGDENTMLPIFIGVFLLGTATLILRKTIKVK | ||||||
Modified residue | 518 | Pentaglycyl murein peptidoglycan amidated alanine | ||||
Sequence: A | ||||||
Propeptide | PRO_0000445901 | 519-548 | Removed by sortase A | |||
Sequence: GDENTMLPIFIGVFLLGTATLILRKTIKVK |
Keywords
- PTM
Proteomic databases
Expression
Induction
More prevalent in stationary than exponential phase (at protein level) (PubMed:20176794, PubMed:21725001).
Single gene transcripts are substantially decreased in a sigB mutant, induced in mouse gut (PubMed:20176794).
Induced as cells approach stationary phase and by growth at pH 5.5, at 45 degrees Celsius, in 7.5% NaCl and by oxidative stress (at protein level) (PubMed:20176794, PubMed:21725001).
Single gene transcripts are substantially decreased in a sigB mutant, induced in mouse gut (PubMed:20176794).
Induced as cells approach stationary phase and by growth at pH 5.5, at 45 degrees Celsius, in 7.5% NaCl and by oxidative stress (at protein level) (PubMed:20176794, PubMed:21725001).
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for repeat, region, motif.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Repeat | 93-105 | LRR 1 | ||||
Sequence: GVQYLNNLIGLEL | ||||||
Repeat | 113-127 | LRR 2 | ||||
Sequence: LTPLKNLTKITELEL | ||||||
Repeat | 135-149 | LRR 3 | ||||
Sequence: VSAIAGLQSIKTLDL | ||||||
Repeat | 157-171 | LRR 4 | ||||
Sequence: VTPLAGLSNLQVLYL | ||||||
Repeat | 179-193 | LRR 5 | ||||
Sequence: ISPLAGLTNLQYLSI | ||||||
Repeat | 201-215 | LRR 6 | ||||
Sequence: LTPLANLSKLTTLKA | ||||||
Repeat | 223-236 | LRR 7 | ||||
Sequence: ISPLASLPNLIEVH | ||||||
Region | 480-518 | Disordered | ||||
Sequence: FTKNDNPNPDDPTTNTPTGNGDGTSNPSNSGGNTTLPTA | ||||||
Motif | 515-519 | LPXTG sorting signal | ||||
Sequence: LPTAG |
Domain
The N-terminus (36-79) resembles a truncated EF hand, the LRR region (80-262) has a curved form while residues 263-343 form an Ig-like region.
Sequence similarities
Belongs to the internalin family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length548
- Mass (Da)58,681
- Last updated2012-05-16 v1
- Checksum3EE34A05CAA1B88F
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AL591974 EMBL· GenBank· DDBJ | CAD00790.1 EMBL· GenBank· DDBJ | Genomic DNA |