Q7A656 · ISDB_STAAN
- ProteinIron-regulated surface determinant protein B
- GeneisdB
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids645 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Cell wall-anchored surface receptor that extracts heme from oxidized methemoglobin/metHb to enable growth on hemoglobin as a sole iron source (PubMed:24645787).
Rapidly extracts heme from hemoglobin and transfers it to IsdA or IsdC, which then relays it to the membrane transporter/IsdEF for internalization (PubMed:21574663, PubMed:24645787, PubMed:29109153).
Promotes also resistance to hydrogen peroxide and killing by neutrophils (By similarity).
Rapidly extracts heme from hemoglobin and transfers it to IsdA or IsdC, which then relays it to the membrane transporter/IsdEF for internalization (PubMed:21574663, PubMed:24645787, PubMed:29109153).
Promotes also resistance to hydrogen peroxide and killing by neutrophils (By similarity).
Biotechnology
A combined vaccine containing IsdA, IsdB, SdrD and SdrE afforded significant protection in mice against a lethal challenge with S.aureus Newman or any of the clinical isolates NRS252, N315, NRS248, USA100 and USA400. The immune response elicited by the combined vaccine is greater than the one elicited by its individual components.
Features
Showing features for binding site.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | extracellular region | |
Molecular Function | heme transmembrane transporter activity | |
Molecular Function | metal ion binding |
Keywords
- Biological process
- Ligand
Names & Taxonomy
Protein names
- Recommended nameIron-regulated surface determinant protein B
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Bacillota > Bacilli > Bacillales > Staphylococcaceae > Staphylococcus
Accessions
- Primary accessionQ7A656
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Secreted, cell wall ; Peptidoglycan-anchor
Note: Anchored to the cell wall by sortase A (By similarity).
Keywords
- Cellular component
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 361 | Severe heme binding disruption. | ||||
Sequence: S → A | ||||||
Mutagenesis | 440 | Severe heme binding disruption. | ||||
Sequence: Y → A | ||||||
Mutagenesis | 440 | Complete loss of heme transfer; in association with F-444. | ||||
Sequence: Y → F | ||||||
Mutagenesis | 444 | Severe heme binding disruption. | ||||
Sequence: Y → A | ||||||
Mutagenesis | 444 | Complete loss of heme transfer; in association with F-440. | ||||
Sequence: Y → F |
Miscellaneous
PTM/Processing
Features
Showing features for signal, chain, modified residue, propeptide.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Signal | 1-40 | |||||
Sequence: MNKQQKEFKSFYSIRKSSLGVASVAISTLLLLMSNGEAQA | ||||||
Chain | PRO_0000292577 | 41-613 | Iron-regulated surface determinant protein B | |||
Sequence: AAEETGGTNTEAQPKTEAVASPTTTSEKAPETKPVANAVSVSNKEVEAPTSETKEAKEVKEVKAPKETKAVKPAAKATNNTYPILNQELREAIKNPAIKDKDHSAPNSRPIDFEMKKENGEQQFYHYASSVKPARVIFTDSKPEIELGLQSGQFWRKFEVYEGDKKLPIKLVSYDTVKDYAYIRFSVSNGTKAVKIVSSTHFNNKEEKYDYTLMEFAQPIYNSADKFKTEEDYKAEKLLAPYKKAKTLERQVYELNKIQDKLPEKLKAEYKKKLEDTKKALDEQVKSAITEFQNVQPTNEKMTDLQDTKYVVYESVENNESMMDTFVKHPIKTGMLNGKKYMVMETTNDDYWKDFMVEGQRVRTISKDAKNNTRTIIFPYVEGKTLYDAIVKVHVKTIDYDGQYHVRIVDKEAFTKANTDKSNKKEQQDNSAKKEATPATPSKPTPSPVEKESQKQDSQKDDNKQLPSVEKENDASSESGKDKTPATKPTKGEVESSSTTPTKVVSTTQNVAKPTTASSKTTKDVVQTSAGSSEAKDSAPLQKANIKNTNDGHTQSQNNKNTQENKAKSLPQT | ||||||
Modified residue | 613 | Pentaglycyl murein peptidoglycan amidated threonine | ||||
Sequence: T | ||||||
Propeptide | PRO_0000292578 | 614-645 | Removed by sortase | |||
Sequence: GEESNKDMTLPLMALLALSSIVAFVLPRKRKN |
Keywords
- PTM
Expression
Induction
Repressed by fur in the presence of iron.
Interaction
Subunit
Interacts with host HBA; this interaction allows heme extraction as iron source. Interacts with IsdA (By similarity).
Structure
Family & Domains
Features
Showing features for motif, region, compositional bias, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Motif | 12-23 | YSIRK-G/S signaling motif | ||||
Sequence: YSIRKSSLGVAS | ||||||
Region | 38-113 | Disordered | ||||
Sequence: AQAAAEETGGTNTEAQPKTEAVASPTTTSEKAPETKPVANAVSVSNKEVEAPTSETKEAKEVKEVKAPKETKAVKP | ||||||
Compositional bias | 41-87 | Polar residues | ||||
Sequence: AAEETGGTNTEAQPKTEAVASPTTTSEKAPETKPVANAVSVSNKEVE | ||||||
Compositional bias | 88-110 | Basic and acidic residues | ||||
Sequence: APTSETKEAKEVKEVKAPKETKA | ||||||
Domain | 144-269 | NEAT 1 | ||||
Sequence: SAPNSRPIDFEMKKENGEQQFYHYASSVKPARVIFTDSKPEIELGLQSGQFWRKFEVYEGDKKLPIKLVSYDTVKDYAYIRFSVSNGTKAVKIVSSTHFNNKEEKYDYTLMEFAQPIYNSADKFKT | ||||||
Domain | 341-458 | NEAT 2 | ||||
Sequence: KMTDLQDTKYVVYESVENNESMMDTFVKHPIKTGMLNGKKYMVMETTNDDYWKDFMVEGQRVRTISKDAKNNTRTIIFPYVEGKTLYDAIVKVHVKTIDYDGQYHVRIVDKEAFTKAN | ||||||
Compositional bias | 458-472 | Basic and acidic residues | ||||
Sequence: NTDKSNKKEQQDNSA | ||||||
Region | 458-619 | Disordered | ||||
Sequence: NTDKSNKKEQQDNSAKKEATPATPSKPTPSPVEKESQKQDSQKDDNKQLPSVEKENDASSESGKDKTPATKPTKGEVESSSTTPTKVVSTTQNVAKPTTASSKTTKDVVQTSAGSSEAKDSAPLQKANIKNTNDGHTQSQNNKNTQENKAKSLPQTGEESNK | ||||||
Compositional bias | 473-492 | Polar residues | ||||
Sequence: KKEATPATPSKPTPSPVEKE | ||||||
Compositional bias | 493-529 | Basic and acidic residues | ||||
Sequence: SQKQDSQKDDNKQLPSVEKENDASSESGKDKTPATKP | ||||||
Compositional bias | 530-619 | Polar residues | ||||
Sequence: TKGEVESSSTTPTKVVSTTQNVAKPTTASSKTTKDVVQTSAGSSEAKDSAPLQKANIKNTNDGHTQSQNNKNTQENKAKSLPQTGEESNK | ||||||
Motif | 610-614 | LPXTG sorting signal | ||||
Sequence: LPQTG |
Domain
The two NEAr transporter (NEAT) domains act in concert to bind, extract, and transfer heme from hemoglobin to downstream Isd proteins.
Sequence similarities
Belongs to the IsdB family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length645
- Mass (Da)72,162
- Last updated2004-07-05 v1
- Checksum524BF70EC0C4F9D8
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 41-87 | Polar residues | ||||
Sequence: AAEETGGTNTEAQPKTEAVASPTTTSEKAPETKPVANAVSVSNKEVE | ||||||
Compositional bias | 88-110 | Basic and acidic residues | ||||
Sequence: APTSETKEAKEVKEVKAPKETKA | ||||||
Compositional bias | 458-472 | Basic and acidic residues | ||||
Sequence: NTDKSNKKEQQDNSA | ||||||
Compositional bias | 473-492 | Polar residues | ||||
Sequence: KKEATPATPSKPTPSPVEKE | ||||||
Compositional bias | 493-529 | Basic and acidic residues | ||||
Sequence: SQKQDSQKDDNKQLPSVEKENDASSESGKDKTPATKP | ||||||
Compositional bias | 530-619 | Polar residues | ||||
Sequence: TKGEVESSSTTPTKVVSTTQNVAKPTTASSKTTKDVVQTSAGSSEAKDSAPLQKANIKNTNDGHTQSQNNKNTQENKAKSLPQTGEESNK |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
BA000018 EMBL· GenBank· DDBJ | BAB42225.1 EMBL· GenBank· DDBJ | Genomic DNA |