Q7A656 · ISDB_STAAN

Function

function

Cell wall-anchored surface receptor that extracts heme from oxidized methemoglobin/metHb to enable growth on hemoglobin as a sole iron source (PubMed:24645787).
Rapidly extracts heme from hemoglobin and transfers it to IsdA or IsdC, which then relays it to the membrane transporter/IsdEF for internalization (PubMed:21574663, PubMed:24645787, PubMed:29109153).
Promotes also resistance to hydrogen peroxide and killing by neutrophils (By similarity).

Biotechnology

A combined vaccine containing IsdA, IsdB, SdrD and SdrE afforded significant protection in mice against a lethal challenge with S.aureus Newman or any of the clinical isolates NRS252, N315, NRS248, USA100 and USA400. The immune response elicited by the combined vaccine is greater than the one elicited by its individual components.

Features

Showing features for binding site.

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TypeIDPosition(s)Description
Binding site362Fe (UniProtKB | ChEBI) of heme (UniProtKB | ChEBI); axial binding residue
Binding site440Fe (UniProtKB | ChEBI) of heme (UniProtKB | ChEBI); axial binding residue

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentextracellular region
Molecular Functionheme transmembrane transporter activity
Molecular Functionmetal ion binding

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Iron-regulated surface determinant protein B
  • Alternative names
    • Fur-regulated protein B
    • Staphylococcal iron-regulated protein H
    • Staphylococcus aureus surface protein J

Gene names

    • Name
      isdB
    • Synonyms
      frpB, sasJ, sirH
    • Ordered locus names
      SA0976

Organism names

Accessions

  • Primary accession
    Q7A656

Proteomes

Subcellular Location

Secreted, cell wall
; Peptidoglycan-anchor
Note: Anchored to the cell wall by sortase A (By similarity).

Keywords

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis361Severe heme binding disruption.
Mutagenesis440Severe heme binding disruption.
Mutagenesis440Complete loss of heme transfer; in association with F-444.
Mutagenesis444Severe heme binding disruption.
Mutagenesis444Complete loss of heme transfer; in association with F-440.

Miscellaneous

PTM/Processing

Features

Showing features for signal, chain, modified residue, propeptide.

TypeIDPosition(s)Description
Signal1-40
ChainPRO_000029257741-613Iron-regulated surface determinant protein B
Modified residue613Pentaglycyl murein peptidoglycan amidated threonine
PropeptidePRO_0000292578614-645Removed by sortase

Keywords

Expression

Induction

Repressed by fur in the presence of iron.

Interaction

Subunit

Interacts with host HBA; this interaction allows heme extraction as iron source. Interacts with IsdA (By similarity).

Family & Domains

Features

Showing features for motif, region, compositional bias, domain.

TypeIDPosition(s)Description
Motif12-23YSIRK-G/S signaling motif
Region38-113Disordered
Compositional bias41-87Polar residues
Compositional bias88-110Basic and acidic residues
Domain144-269NEAT 1
Domain341-458NEAT 2
Compositional bias458-472Basic and acidic residues
Region458-619Disordered
Compositional bias473-492Polar residues
Compositional bias493-529Basic and acidic residues
Compositional bias530-619Polar residues
Motif610-614LPXTG sorting signal

Domain

The two NEAr transporter (NEAT) domains act in concert to bind, extract, and transfer heme from hemoglobin to downstream Isd proteins.

Sequence similarities

Belongs to the IsdB family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    645
  • Mass (Da)
    72,162
  • Last updated
    2004-07-05 v1
  • Checksum
    524BF70EC0C4F9D8
MNKQQKEFKSFYSIRKSSLGVASVAISTLLLLMSNGEAQAAAEETGGTNTEAQPKTEAVASPTTTSEKAPETKPVANAVSVSNKEVEAPTSETKEAKEVKEVKAPKETKAVKPAAKATNNTYPILNQELREAIKNPAIKDKDHSAPNSRPIDFEMKKENGEQQFYHYASSVKPARVIFTDSKPEIELGLQSGQFWRKFEVYEGDKKLPIKLVSYDTVKDYAYIRFSVSNGTKAVKIVSSTHFNNKEEKYDYTLMEFAQPIYNSADKFKTEEDYKAEKLLAPYKKAKTLERQVYELNKIQDKLPEKLKAEYKKKLEDTKKALDEQVKSAITEFQNVQPTNEKMTDLQDTKYVVYESVENNESMMDTFVKHPIKTGMLNGKKYMVMETTNDDYWKDFMVEGQRVRTISKDAKNNTRTIIFPYVEGKTLYDAIVKVHVKTIDYDGQYHVRIVDKEAFTKANTDKSNKKEQQDNSAKKEATPATPSKPTPSPVEKESQKQDSQKDDNKQLPSVEKENDASSESGKDKTPATKPTKGEVESSSTTPTKVVSTTQNVAKPTTASSKTTKDVVQTSAGSSEAKDSAPLQKANIKNTNDGHTQSQNNKNTQENKAKSLPQTGEESNKDMTLPLMALLALSSIVAFVLPRKRKN

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias41-87Polar residues
Compositional bias88-110Basic and acidic residues
Compositional bias458-472Basic and acidic residues
Compositional bias473-492Polar residues
Compositional bias493-529Basic and acidic residues
Compositional bias530-619Polar residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
BA000018
EMBL· GenBank· DDBJ
BAB42225.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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