Q75VR0 · TPC1B_TOBAC
- ProteinTwo pore calcium channel protein 1B
- GeneTPC1B
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids735 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score4/5
Function
function
Functions as a voltage-gated inward-rectifying Ca2+ channel (VDCC) across the plasma membrane that mediates sucrose-induced Ca2+ influx in autotrophically grown leaf cells. Acts as the major ROS-responsive Ca2+ channel and is the possible target of Al-dependent inhibition. Plays a regulatory role in defense responses.
Miscellaneous
Rescues the Ca2+ uptake activity in yeast mutant cch1.
Activity regulation
Inhibited by Al3+, La3+ and Gd3+. Up-regulated by H2O2, cryptogein, salicylic acid (SA) and cold shock.
GO annotations
all annotations | all molecular function | nucleotide binding | molecular_function | nucleic acid binding | dna binding | chromatin binding | dna-binding transcription factor activity | rna binding | cytoskeletal motor activity | catalytic activity | nuclease activity | signaling receptor binding | structural molecule activity | transporter activity | binding | protein binding | translation factor activity, rna binding | lipid binding | kinase activity | transferase activity | hydrolase activity | oxygen binding | enzyme regulator activity | carbohydrate binding | signaling receptor activity | translation regulator activity | transcription regulator activity | other molecular function | all biological process | carbohydrate metabolic process | generation of precursor metabolites and energy | nucleobase-containing compound metabolic process | dna metabolic process | translation | lipid metabolic process | transport | response to stress | cell cycle | cell communication | signal transduction | cell-cell signaling | multicellular organism development | circadian rhythm | biological_process | metabolic process | catabolic process | biosynthetic process | response to light stimulus | response to external stimulus | tropism | response to biotic stimulus | response to abiotic stimulus | response to endogenous stimulus | embryo development | post-embryonic development | fruit ripening | abscission | pollination | flower development | cellular process | programmed cell death | photosynthesis | cellular component organization | cell growth | protein metabolic process | cellular homeostasis | secondary metabolic process | reproductive process | cell differentiation | protein modification process | growth | epigenetic regulation of gene expression | response to chemical | anatomical structure development | regulation of molecular function | other biological process | all cellular component | cellular_component | extracellular region | cell wall | intracellular anatomical structure | nucleus | nuclear envelope | nucleoplasm | nucleolus | cytoplasm | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | cytosol | ribosome | cytoskeleton | plasma membrane | chloroplast | plastid | thylakoid | membrane | external encapsulating structure | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | monoatomic ion channel complex | |
Cellular Component | plant-type vacuole | |
Cellular Component | vacuolar membrane | |
Molecular Function | calcium ion binding | |
Molecular Function | voltage-gated calcium channel activity | |
Biological Process | calcium ion transport | |
Biological Process | defense response |
Keywords
- Molecular function
- Biological process
- Ligand
Names & Taxonomy
Protein names
- Recommended nameTwo pore calcium channel protein 1B
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > asterids > lamiids > Solanales > Solanaceae > Nicotianoideae > Nicotianeae > Nicotiana
Accessions
- Primary accessionQ75VR0
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Membrane ; Multi-pass membrane protein
Features
Showing features for topological domain, transmembrane, intramembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-76 | Cytoplasmic | ||||
Sequence: MEEYLLPGESSNSCRTRRRSGSIFDRRDAIAHGSAYQKAAALVDLAEDGIGLPEEILEGASFEKAAELYFMFTRFD | ||||||
Transmembrane | 77-97 | Helical; Name=S1 of repeat I | ||||
Sequence: FLWSLNYLALVVLNFFEKPLW | ||||||
Topological domain | 98-125 | Extracellular | ||||
Sequence: CSKHLAESCNNRDYYYLGELPFLTGAES | ||||||
Transmembrane | 126-146 | Helical; Name=S2 of repeat I | ||||
Sequence: LIFEGVTLLLLIIHILFPISY | ||||||
Topological domain | 147-161 | Cytoplasmic | ||||
Sequence: EGFNLYWRSLLNRLK | ||||||
Transmembrane | 162-182 | Helical; Name=S3 of repeat I | ||||
Sequence: VILLLILVADIVVYILLPADF | ||||||
Topological domain | 183 | Extracellular | ||||
Sequence: Y | ||||||
Transmembrane | 184-202 | Helical; Voltage-sensor; Name=S4 of repeat I | ||||
Sequence: YLPFRIAPYLRVVFFILNI | ||||||
Topological domain | 203-208 | Cytoplasmic | ||||
Sequence: RELRDS | ||||||
Transmembrane | 209-229 | Helical; Name=S5 of repeat I | ||||
Sequence: FFILAGMLGTYLNVVALSALF | ||||||
Topological domain | 230-248 | Extracellular | ||||
Sequence: LLFSSWLAYVFFEDTRQGK | ||||||
Intramembrane | 249-263 | Pore-forming; Name=Pore-forming 1 | ||||
Sequence: TTFTSYGTTLYQMFV | ||||||
Topological domain | 264-286 | Extracellular | ||||
Sequence: LFTTSNNPDVWIPAYKDSRWYCL | ||||||
Transmembrane | 287-307 | Helical; Name=S6 of repeat I | ||||
Sequence: FFVLYVLLGVYFVTNLILAVV | ||||||
Topological domain | 308-431 | Cytoplasmic | ||||
Sequence: YDSFKSELVKQVADKDRLRLRTLKKAFSLIDEANNGLLNEKQCTLLFEELNKYRTLPKISGDDFKSIFNELDDTGDFKINLEEFADLCSAIGLRFQKEDSLPIFEACPNFYHSPASEKLRGFIR | ||||||
Transmembrane | 432-452 | Helical; Name=S1 of repeat II | ||||
Sequence: GATFEYIIVFVLLVNLVAVII | ||||||
Topological domain | 453-470 | Extracellular | ||||
Sequence: ETTLDIQNNSGQTFWQKV | ||||||
Transmembrane | 471-491 | Helical; Name=S2 of repeat II | ||||
Sequence: EFTFGWLYVIEMALKVYTYGF | ||||||
Topological domain | 492-501 | Cytoplasmic | ||||
Sequence: ENYWRDGQNR | ||||||
Transmembrane | 502-522 | Helical; Name=S3 of repeat II | ||||
Sequence: FDFIVTWVIVIGETTTFVAPD | ||||||
Topological domain | 523-531 | Extracellular | ||||
Sequence: DLTFLSNGE | ||||||
Transmembrane | 532-549 | Helical; Voltage-sensor; Name=S4 of repeat II | ||||
Sequence: WIRYLLIARMLRLIRLLM | ||||||
Topological domain | 550-560 | Cytoplasmic | ||||
Sequence: HVERYRAFVAT | ||||||
Transmembrane | 561-581 | Helical; Name=S5 of repeat II | ||||
Sequence: FLTLIPSLMPYLGTIFCILCF | ||||||
Topological domain | 582-618 | Extracellular | ||||
Sequence: YCSLGLQIFGGIVNTGNPNLAQTDLAGNDYLLFNFND | ||||||
Intramembrane | 619-633 | Pore-forming; Name=Pore-forming 2 | ||||
Sequence: YPNGMVTLFNILVMG | ||||||
Topological domain | 634-654 | Extracellular | ||||
Sequence: NWQVWMQSYKELTGTSWTYAY | ||||||
Transmembrane | 655-675 | Helical; Name=S6 of repeat II | ||||
Sequence: FVSFYLISVLWLLNLIVAFVL | ||||||
Topological domain | 676-735 | Cytoplasmic | ||||
Sequence: EAFQAEMDLEASARCVDGDDKEAKRERRRNVGTKTRSQRVDFLLHHMLRSELTECSNDNP |
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain, glycosylation.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000343172 | 1-735 | Two pore calcium channel protein 1B | |||
Sequence: MEEYLLPGESSNSCRTRRRSGSIFDRRDAIAHGSAYQKAAALVDLAEDGIGLPEEILEGASFEKAAELYFMFTRFDFLWSLNYLALVVLNFFEKPLWCSKHLAESCNNRDYYYLGELPFLTGAESLIFEGVTLLLLIIHILFPISYEGFNLYWRSLLNRLKVILLLILVADIVVYILLPADFYYLPFRIAPYLRVVFFILNIRELRDSFFILAGMLGTYLNVVALSALFLLFSSWLAYVFFEDTRQGKTTFTSYGTTLYQMFVLFTTSNNPDVWIPAYKDSRWYCLFFVLYVLLGVYFVTNLILAVVYDSFKSELVKQVADKDRLRLRTLKKAFSLIDEANNGLLNEKQCTLLFEELNKYRTLPKISGDDFKSIFNELDDTGDFKINLEEFADLCSAIGLRFQKEDSLPIFEACPNFYHSPASEKLRGFIRGATFEYIIVFVLLVNLVAVIIETTLDIQNNSGQTFWQKVEFTFGWLYVIEMALKVYTYGFENYWRDGQNRFDFIVTWVIVIGETTTFVAPDDLTFLSNGEWIRYLLIARMLRLIRLLMHVERYRAFVATFLTLIPSLMPYLGTIFCILCFYCSLGLQIFGGIVNTGNPNLAQTDLAGNDYLLFNFNDYPNGMVTLFNILVMGNWQVWMQSYKELTGTSWTYAYFVSFYLISVLWLLNLIVAFVLEAFQAEMDLEASARCVDGDDKEAKRERRRNVGTKTRSQRVDFLLHHMLRSELTECSNDNP | ||||||
Glycosylation | 460 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N |
Keywords
- PTM
Proteomic databases
PTM databases
Interaction
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 325-360 | EF-hand 1 | ||||
Sequence: LRLRTLKKAFSLIDEANNGLLNEKQCTLLFEELNKY | ||||||
Domain | 366-401 | EF-hand 2 | ||||
Sequence: ISGDDFKSIFNELDDTGDFKINLEEFADLCSAIGLR |
Domain
Each of the two internal repeats contains five hydrophobic transmembrane segments (S1, S2, S3, S5, S6) and one positively charged transmembrane segment (S4). S4 segments probably represent the voltage-sensor and are characterized by a series of positively charged amino acids (By similarity).
Sequence similarities
Belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family. Two pore calcium channel subfamily.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length735
- Mass (Da)85,147
- Last updated2004-07-05 v1
- Checksum9E52EE2769D451B8
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AB124647 EMBL· GenBank· DDBJ | BAD15100.1 EMBL· GenBank· DDBJ | mRNA |