Q757M7 · SIR2_EREGS
- ProteinNAD-dependent histone deacetylase SIR2
- GeneSIR2
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids559 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
NAD-dependent deacetylase. Heterochromatin component that silences transcription at silent mating loci, telomeres and the ribosomal DNA, and that also suppresses recombination in the rDNA and extends replicative life span. It acts as a NAD-dependent histone deacetylase, which deacetylates 'Lys-9' and 'Lys-14' of Histone H3 and 'Lys-16' of Histone H4 (By similarity).
Catalytic activity
- H2O + N6-acetyl-L-lysyl-[protein] + NAD+ = 2''-O-acetyl-ADP-D-ribose + L-lysyl-[protein] + nicotinamide
Cofactor
Note: Binds 1 zinc ion per subunit.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 248-267 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: GAGISTSLGIPDFRSSKGFY | ||||||
Binding site | 330-333 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: QNID | ||||||
Active site | 350 | Proton acceptor | ||||
Sequence: H | ||||||
Binding site | 358 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 361 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 382 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 385 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 458-460 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: GTS | ||||||
Binding site | 483-485 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: NRD | ||||||
Binding site | 500 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: C |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | nucleus | |
Cellular Component | Set3 complex | |
Molecular Function | metal ion binding | |
Molecular Function | NAD+ binding | |
Molecular Function | NAD-dependent histone H4K16 deacetylase activity | |
Molecular Function | transferase activity | |
Biological Process | negative regulation of meiotic nuclear division | |
Biological Process | negative regulation of mitotic recombination | |
Biological Process | regulation of thiamine biosynthetic process | |
Biological Process | silent mating-type cassette heterochromatin formation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameNAD-dependent histone deacetylase SIR2
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Saccharomycotina > Saccharomycetes > Saccharomycetales > Saccharomycetaceae > Eremothecium
Accessions
- Primary accessionQ757M7
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000110274 | 1-559 | NAD-dependent histone deacetylase SIR2 | |||
Sequence: MSESASMLQGSKRGTDSSVDVGGAKRTKVDMDDGSSSNNDEKELLEATKADELDEVVDDYAEQNDHSAQEVAGEYVPKKPEQPIMLTKDSNSGKYVFPPISKEDSLNARYFLKYHGSAQFLDSYLPEDLNSLYVFHLIKLLGFQLKDRELLTAVQKAVQNDVTSGVSSVVGTNPMVERVSPPQLSASTPSGDGYDDPLEKKHAVRLIKDLQKAMNKVLSTRIRLANFFTLDHFISKLKSAKKVLVLTGAGISTSLGIPDFRSSKGFYSQVTNLGLDDPQDVFNLDIFMENPSVFYTIAEKILPPEHKFSPLHSFIKMIQDKGKLLRNYTQNIDNLESYAGIFKENIVQCHGSFATASCVTCHLKMPGERIFQQIKDREIPLCAYCYPKRQEEYPTVSDDPGTKNGQQSSHNSSSIFHMSRSFGVIKPDITFFGEALPLEFHTNIRQDVLQCDLLICIGTSLKVAPVSEIVNMVPAHVPQVLINRDPVKHAEFDLTLLGLCDDVAAFIAQKCGWDIPHENWPQLKQRNIQYDELERGMYYVYDPVKEQAKDTGQRQVQAP |
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for compositional bias, region, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 1-16 | Polar residues | ||||
Sequence: MSESASMLQGSKRGTD | ||||||
Region | 1-73 | Disordered | ||||
Sequence: MSESASMLQGSKRGTDSSVDVGGAKRTKVDMDDGSSSNNDEKELLEATKADELDEVVDDYAEQNDHSAQEVAG | ||||||
Compositional bias | 23-51 | Basic and acidic residues | ||||
Sequence: GAKRTKVDMDDGSSSNNDEKELLEATKAD | ||||||
Domain | 223-514 | Deacetylase sirtuin-type | ||||
Sequence: RLANFFTLDHFISKLKSAKKVLVLTGAGISTSLGIPDFRSSKGFYSQVTNLGLDDPQDVFNLDIFMENPSVFYTIAEKILPPEHKFSPLHSFIKMIQDKGKLLRNYTQNIDNLESYAGIFKENIVQCHGSFATASCVTCHLKMPGERIFQQIKDREIPLCAYCYPKRQEEYPTVSDDPGTKNGQQSSHNSSSIFHMSRSFGVIKPDITFFGEALPLEFHTNIRQDVLQCDLLICIGTSLKVAPVSEIVNMVPAHVPQVLINRDPVKHAEFDLTLLGLCDDVAAFIAQKCGWD |
Sequence similarities
Belongs to the sirtuin family. Class I subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length559
- Mass (Da)62,561
- Last updated2004-07-05 v1
- ChecksumC8817AE0DC14D24C
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 1-16 | Polar residues | ||||
Sequence: MSESASMLQGSKRGTD | ||||||
Compositional bias | 23-51 | Basic and acidic residues | ||||
Sequence: GAKRTKVDMDDGSSSNNDEKELLEATKAD |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AE016818 EMBL· GenBank· DDBJ | AAS52672.1 EMBL· GenBank· DDBJ | Genomic DNA |