Q756Y6 · Q756Y6_EREGS

Function

function

DNA-dependent ATPase and 5'-3' DNA helicase required for the maintenance of both mitochondrial and nuclear genome stability. Efficiently unwinds G-quadruplex (G4) DNA structures and forked RNA-DNA hybrids. Resolves G4 structures, preventing replication pausing and double-strand breaks (DSBs) at G4 motifs. Involved in the maintenance of telomeric DNA. Inhibits telomere elongation, de novo telomere formation and telomere addition to DSBs via catalytic inhibition of telomerase. Reduces the processivity of telomerase by displacing active telomerase from DNA ends. Releases telomerase by unwinding the short telomerase RNA/telomeric DNA hybrid that is the intermediate in the telomerase reaction. Involved in the maintenance of ribosomal (rDNA). Required for efficient fork arrest at the replicaion fork barrier within rDNA. Involved in the maintenance of mitochondrial (mtDNA). Required to maintain mtDNA under conditions that introduce dsDNA breaks in mtDNA, either preventing or repairing dsDNA breaks. May inhibit replication progression to allow time for repair. May have a general role in chromosomal replication by affecting Okazaki fragment maturation. May have a role in conjunction with DNA2 helicase/nuclease in 5'-flap extension during Okazaki fragment processing.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Features

Showing features for binding site, dna binding.

TypeIDPosition(s)Description
Binding site218-225ATP (UniProtKB | ChEBI)
DNA binding689-708

GO annotations

AspectTerm
Cellular Componentmitochondrial membrane
Cellular Componentmitochondrion
Cellular Componentnuclear replication fork
Molecular Function5'-3' DNA helicase activity
Molecular FunctionATP binding
Molecular FunctionATP hydrolysis activity
Molecular FunctionG-quadruplex DNA binding
Molecular Functionsingle-stranded DNA binding
Molecular Functiontelomerase inhibitor activity
Biological ProcessDNA duplex unwinding
Biological ProcessDNA replication
Biological ProcessDNA unwinding involved in DNA replication
Biological Processdouble-strand break repair via break-induced replication
Biological ProcessG-quadruplex DNA unwinding
Biological Processmitochondrial genome maintenance
Biological Processnegative regulation of telomere maintenance via telomerase
Biological Processreplication fork reversal
Biological Processtelomere maintenance
Biological Processtelomere maintenance via recombination

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    ATP-dependent DNA helicase PIF1
  • EC number
  • Alternative names
    • DNA repair and recombination helicase PIF1

Gene names

    • Name
      PIF1
    • ORF names
      AGOS_AER128W

Organism names

Accessions

  • Primary accession
    Q756Y6

Proteomes

Subcellular Location

Keywords

Interaction

Subunit

Monomer. Interacts with telomerase.

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain, region.

TypeIDPosition(s)Description
Domain210-358AAA+ ATPase
Region778-802Disordered

Sequence similarities

Belongs to the helicase family. PIF1 subfamily.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    802
  • Mass (Da)
    89,967
  • Last updated
    2004-07-05 v1
  • Checksum
    901A75489EBF306F
MLKYCRQLKTLHALQVRGTIHAGSKSYLVMKNRLEGSWAFNSDAKRFKHDGSMVQELEGEGKRGNASVLEEAWDTSGDDDILQHIAALEERRGEPDPSMCSGVASGTRLEVQMQRTEAEIKQSKEAPEPLIELPDVQILGSKTVDSKESIKNSAAGTQELAFLTQRPTLHEHISADQPQASDQRPSASPKRVQCFMLSDEQRNVLKLAEEGTNIFYTGSAGTGKSILLRELIKKLKTVHGRQGAVAVTASTGLAACNIGGITVHSFAGIGLGNSPKENLLKKVKRSRKHVQRWQNCKCLIIDEISMIDGELLDKLDYIARKIKKSKAPFGGIQLILCGDFFQLPPVTRDNQQPNFAFDSEAWKQAITATIVLTRVYRQQGDTKFIQMLNDMRLGKISEETEAEFRKLSRPLVTDDIIPAQLFSTRVEVERANGSQLAKLPGKRRAYNAIDGGDLSDEELKQRLLANFLAPKRLELKVGAQVMNIKNFDDTLVNGSLGKVIDFIDEATFMFYQSVRDDDLDVAEWEKYRDALAKNDWNAPELDEDEEPKKETRTSMVKKALREKFRTIDTDDTVDHLGDTIFDFLKDDREVTDPEVKANLERKKQLLQEVHMSSKGKKLPLVQFFQPDNTTRTVLVREEAWAIEDENEKPLVTRVQLPLMLAWALSIHKSQGQTLNKVKVDLRRVFEKGQAYVALSRAVSRGGLQVLNFDKEKIQAHSKVVEFYDSLVSANDAVKMRKDVQRKISFPITKSKAPNAPTLPLAPQLRGNSIITLLERSNKNSGRSLSQKFSYVPSQSNNQAPSP

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AE016818
EMBL· GenBank· DDBJ
AAS52811.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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