Q74JA6 · ADDA_LACJO
- ProteinATP-dependent helicase/nuclease subunit A
- GeneaddA
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids1204 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
The heterodimer acts as both an ATP-dependent DNA helicase and an ATP-dependent, dual-direction single-stranded exonuclease. Recognizes the chi site generating a DNA molecule suitable for the initiation of homologous recombination. The AddA nuclease domain is required for chi fragment generation; this subunit has the helicase and 3' -> 5' nuclease activities.
Catalytic activity
- ATP + H2O = ADP + phosphate + H+
Cofactor
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 23-30 | ATP (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Cellular Component | DNA helicase complex | |
Molecular Function | 3'-5' DNA helicase activity | |
Molecular Function | 3'-5' exonuclease activity | |
Molecular Function | ATP binding | |
Molecular Function | ATP hydrolysis activity | |
Molecular Function | double-stranded DNA binding | |
Molecular Function | isomerase activity | |
Biological Process | double-strand break repair via homologous recombination |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameATP-dependent helicase/nuclease subunit A
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Bacillota > Bacilli > Lactobacillales > Lactobacillaceae > Lactobacillus
Accessions
- Primary accessionQ74JA6
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000379284 | 1-1204 | ATP-dependent helicase/nuclease subunit A | ||
Interaction
Subunit
Heterodimer of AddA and AddB/RexB.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 2-469 | UvrD-like helicase ATP-binding | |||
Domain | 496-784 | UvrD-like helicase C-terminal | |||
Sequence similarities
Belongs to the helicase family. AddA subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,204
- Mass (Da)139,871
- Last updated2004-07-05 v1
- Checksum6049198AB96EA162
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AE017198 EMBL· GenBank· DDBJ | AAS09024.1 EMBL· GenBank· DDBJ | Genomic DNA |