Q740J9 · LYSX_MYCPA
- ProteinLysylphosphatidylglycerol biosynthesis bifunctional protein LysX
- GenelysX
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids1177 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Catalyzes the production of L-lysyl-tRNA(Lys)transfer and the transfer of a lysyl group from L-lysyl-tRNA(Lys) to membrane-bound phosphatidylglycerol (PG), which produces lysylphosphatidylglycerol (LPG), one of the components of the bacterial membrane with a positive net charge. LPG synthesis contributes to the resistance to cationic antimicrobial peptides (CAMPs) and likely protects M.tuberculosis against the CAMPs produced by competiting microorganisms (bacteriocins). In fact, the modification of anionic phosphatidylglycerol with positively charged L-lysine results in repulsion of the peptides (By similarity).
Catalytic activity
- ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys)
Cofactor
Note: Binds 3 Mg2+ ions per subunit.
Features
Showing features for dna binding, binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | plasma membrane | |
Molecular Function | ATP binding | |
Molecular Function | DNA binding | |
Molecular Function | lysine-tRNA ligase activity | |
Molecular Function | magnesium ion binding | |
Molecular Function | phosphatidylglycerol lysyltransferase activity | |
Biological Process | lipid metabolic process | |
Biological Process | lysyl-tRNA aminoacylation | |
Biological Process | response to antibiotic |
Keywords
- Molecular function
- Biological process
- Ligand
Names & Taxonomy
Protein names
- Recommended nameLysylphosphatidylglycerol biosynthesis bifunctional protein LysX
Including 2 domains:
- Recommended nameLysine--tRNA ligase
- EC number
- Alternative names
- Recommended namePhosphatidylglycerol lysyltransferase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Actinomycetota > Actinomycetes > Mycobacteriales > Mycobacteriaceae > Mycobacterium > Mycobacterium avium complex (MAC)
Accessions
- Primary accessionQ740J9
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Cell membrane ; Multi-pass membrane protein
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transmembrane | 93-113 | Helical | ||||
Sequence: VPAAAGWTVGVIATVSLLGSV | ||||||
Transmembrane | 135-155 | Helical | ||||
Sequence: FPDTSIAWSFVLALLAAALTA | ||||||
Transmembrane | 159-179 | Helical | ||||
Sequence: IAWLLLLGNMILAAALNVADI | ||||||
Transmembrane | 189-209 | Helical | ||||
Sequence: IFGENLGFAVHIVAIVLLVLA | ||||||
Transmembrane | 227-247 | Helical | ||||
Sequence: AVLVAGDVVGILVSWGLVELF | ||||||
Transmembrane | 281-301 | Helical | ||||
Sequence: VFLNAIFGLFGALALIMATIV |
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000394323 | 1-1177 | Lysylphosphatidylglycerol biosynthesis bifunctional protein LysX | |||
Sequence: MRRAGRSRQFSSVEEAFSTSAARPGPRGRRSGRENTAKFVPATLSASTSFSRIEGISSRSVTLASPGSRSGSGPRSGPRLGPRNRSTSRYRWVPAAAGWTVGVIATVSLLGSVSPLIRYLIKVPREFINDYLFNFPDTSIAWSFVLALLAAALTARKRIAWLLLLGNMILAAALNVADIAAGDNTAAEIFGENLGFAVHIVAIVLLVLAYREFWAKVRKGALVKAAAVLVAGDVVGILVSWGLVELFPGTLARQDRLPYVVNRVVGFALADPDLFTGRPHVFLNAIFGLFGALALIMATIVLFQSQRAENALTGEDESAIRGLLELYGKNDSLGYFATRRDKSVVFAQSGRAAITYRVEIGVCLASGDPIGDPRAWPQAVDAWLGLCQTYGWAPGVMGASTQGARTYREAGLNALELGDEAILRTSEFKLSGPDMRGVRQAVTRARRAGLTVRIRRHRDISPEAMADTIARADAWRDTQTERGFSMALGRLGDPADGDCLLVEAIDRDGSVVAMLSLVPWGTTGVSLDLMRRSPSSPNGTIELMVSELALNAESLGITRISLNFAMFRSAFEQGAQLGAGPVARLWRGLLLFFSRWWQLETLYRSNMKYQPDWVPRYACYEDARLIPRVGVASVIAEGFLVLPFSRRDKVHTGHHPAVPARLAQSGLLHHDGSAPDVSGLRPERTDAEEARSRLPEQVRVRLAKLKVLQRNGVDAYPVGCPPSHTVAQALDADDQQDITVAGRILRIRDFGGVLFAQLRDWSGEMQVLLDNSRLERGRTADFTAAIDLGDLVEVSGQMGFSKKGTRSLIVTDWRMIGKCLRPLPNKWKGLTDPEARVRTRYVDLAVNPESRELIAARSEVLRSVRQTLFAKGFIEVETPILQQIHGGATARPFVTHINTYDMDLFLRIAPELYLKRLCVGGVERVFELGRAFRNEGVDFSHNPEFTLLEAYQAHADYRVWIDGCRELIQNAAQAAHGEQTVLRPGADGRLQPVDISGIWAVKTVHDAVSEALGEQVDPGTSLSTLRKLSDAARIPYRAHWDAGAVVLELYEHLVEDRTEEPTFYVDFPTSVSPLTRPHRSRPGVAERWDLVAWGVELATAYSELTDPVEQRRRLQEQSLLAAGGDPEAMELDEDFLQAMEYAMPPTGGLGMGVDRLVMLITGRSIRETLPFPLAKPH |
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-40 | Disordered | ||||
Sequence: MRRAGRSRQFSSVEEAFSTSAARPGPRGRRSGRENTAKFV | ||||||
Region | 1-676 | Phosphatidylglycerol lysyltransferase | ||||
Sequence: MRRAGRSRQFSSVEEAFSTSAARPGPRGRRSGRENTAKFVPATLSASTSFSRIEGISSRSVTLASPGSRSGSGPRSGPRLGPRNRSTSRYRWVPAAAGWTVGVIATVSLLGSVSPLIRYLIKVPREFINDYLFNFPDTSIAWSFVLALLAAALTARKRIAWLLLLGNMILAAALNVADIAAGDNTAAEIFGENLGFAVHIVAIVLLVLAYREFWAKVRKGALVKAAAVLVAGDVVGILVSWGLVELFPGTLARQDRLPYVVNRVVGFALADPDLFTGRPHVFLNAIFGLFGALALIMATIVLFQSQRAENALTGEDESAIRGLLELYGKNDSLGYFATRRDKSVVFAQSGRAAITYRVEIGVCLASGDPIGDPRAWPQAVDAWLGLCQTYGWAPGVMGASTQGARTYREAGLNALELGDEAILRTSEFKLSGPDMRGVRQAVTRARRAGLTVRIRRHRDISPEAMADTIARADAWRDTQTERGFSMALGRLGDPADGDCLLVEAIDRDGSVVAMLSLVPWGTTGVSLDLMRRSPSSPNGTIELMVSELALNAESLGITRISLNFAMFRSAFEQGAQLGAGPVARLWRGLLLFFSRWWQLETLYRSNMKYQPDWVPRYACYEDARLIPRVGVASVIAEGFLVLPFSRRDKVHTGHHPAVPARLAQSGLLHHDGSAPD | ||||||
Region | 61-85 | Disordered | ||||
Sequence: VTLASPGSRSGSGPRSGPRLGPRNR | ||||||
Region | 673-693 | Disordered | ||||
Sequence: SAPDVSGLRPERTDAEEARSR | ||||||
Region | 677-1177 | Lysine--tRNA ligase | ||||
Sequence: VSGLRPERTDAEEARSRLPEQVRVRLAKLKVLQRNGVDAYPVGCPPSHTVAQALDADDQQDITVAGRILRIRDFGGVLFAQLRDWSGEMQVLLDNSRLERGRTADFTAAIDLGDLVEVSGQMGFSKKGTRSLIVTDWRMIGKCLRPLPNKWKGLTDPEARVRTRYVDLAVNPESRELIAARSEVLRSVRQTLFAKGFIEVETPILQQIHGGATARPFVTHINTYDMDLFLRIAPELYLKRLCVGGVERVFELGRAFRNEGVDFSHNPEFTLLEAYQAHADYRVWIDGCRELIQNAAQAAHGEQTVLRPGADGRLQPVDISGIWAVKTVHDAVSEALGEQVDPGTSLSTLRKLSDAARIPYRAHWDAGAVVLELYEHLVEDRTEEPTFYVDFPTSVSPLTRPHRSRPGVAERWDLVAWGVELATAYSELTDPVEQRRRLQEQSLLAAGGDPEAMELDEDFLQAMEYAMPPTGGLGMGVDRLVMLITGRSIRETLPFPLAKPH |
Sequence similarities
In the N-terminal section; belongs to the LPG synthetase family.
In the C-terminal section; belongs to the class-II aminoacyl-tRNA synthetase family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,177
- Mass (Da)129,146
- Last updated2004-07-05 v1
- Checksum6FE2FE4A144BC8FF
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AE016958 EMBL· GenBank· DDBJ | AAS03668.1 EMBL· GenBank· DDBJ | Genomic DNA |