Q73XV3 · MBTI_MYCPA
- ProteinSalicylate synthase
- GenembtI
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids450 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Involved in the incorporation of salicylate into the virulence-conferring salicylate-based siderophore mycobactin. Catalyzes the initial conversion of chorismate to yield the intermediate isochorismate (isochorismate synthase activity), and the subsequent elimination of the enolpyruvyl side chain in a lyase reaction to give salicylate (isochorismate pyruvate-lyase activity). In the absence of magnesium, MbtI displays a chorismate mutase activity and converts chorismate to prephenate.
Catalytic activity
- chorismate = isochorismate
Cofactor
Pathway
Siderophore biosynthesis; mycobactin biosynthesis.
Features
Showing features for active site, binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 252 | Proton donor | ||||
Sequence: E | ||||||
Binding site | 270-271 | substrate | ||||
Sequence: GT | ||||||
Binding site | 297 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 385 | substrate | ||||
Sequence: Y | ||||||
Binding site | 405 | substrate | ||||
Sequence: R | ||||||
Binding site | 419-421 | substrate | ||||
Sequence: GAG | ||||||
Binding site | 431 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 434 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 438 | substrate | ||||
Sequence: K |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | chorismate mutase activity | |
Molecular Function | isochorismate pyruvate lyase activity | |
Molecular Function | isochorismate synthase activity | |
Molecular Function | magnesium ion binding | |
Molecular Function | oxo-acid-lyase activity | |
Biological Process | catechol-containing siderophore biosynthetic process | |
Biological Process | salicylic acid biosynthetic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameSalicylate synthase
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Actinomycetota > Actinomycetes > Mycobacteriales > Mycobacteriaceae > Mycobacterium > Mycobacterium avium complex (MAC)
Accessions
- Primary accessionQ73XV3
Proteomes
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000262085 | 1-450 | Salicylate synthase | |||
Sequence: MTEVSVETTSAGSESPSIPLPVHIDPADLAAELAVVLSERAGEEYLLYERGGEWVLATGVRAMIELDSDELRVIRDGVTQRQHWSGRPGPVLGEAIDRLLLETDQLFGWIAFEFGVYRYGLQQRLAPGTALARVFWPNGRIVVTREAIQLFGTSTGRRDDVLGVLGDGVPGLRDASAVDVVTDPSNYRDRVASAVAEIAAGRYHKVILSRCLQVPFAVDFPSTYRLARRHNTPVRSFLLRLGGIRAVGYSPELVAAVRHDGVVVTEPLAGTRAFGRGALHDRQARDDLESNSKEIVEHAISVRSSLQEMAEIAEPGTAVVTDFMTVRERGSVQHLGSTVSGRLGTSNDRMDALEALFPAVTASGIPKAGGVEAILRLDEGPRGLYSGAVVMVSADGALDAALTLRAAYEHDGKTWLRAGAGIIEESTPEREFEETCEKLSTLAPYLIARQ |
Interaction
Structure
Sequence
- Sequence statusComplete
- Length450
- Mass (Da)48,635
- Last updated2004-07-05 v1
- Checksum93C76A2A3E1A2E24
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AE016958 EMBL· GenBank· DDBJ | AAS04522.1 EMBL· GenBank· DDBJ | Genomic DNA |