Q729A3 · METK_NITV2

Function

function

Catalyzes the formation of S-adenosylmethionine (AdoMet) from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the enzyme.

Catalytic activity

Cofactor

Protein has several cofactor binding sites:
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Binds 2 divalent ions per subunit.
K+ (UniProtKB | Rhea| CHEBI:29103 )

Note: Binds 1 potassium ion per subunit.

Pathway

Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis; S-adenosyl-L-methionine from L-methionine: step 1/1.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site19ATP (UniProtKB | ChEBI); ligand shared between two neighboring subunits; in other chain
Binding site21Mg2+ (UniProtKB | ChEBI)
Binding site47K+ (UniProtKB | ChEBI)
Binding site60L-methionine (UniProtKB | ChEBI); ligand shared between two neighboring subunits; in other chain
Binding site103L-methionine (UniProtKB | ChEBI); ligand shared between two neighboring subunits; in other chain
Binding site168-170ATP (UniProtKB | ChEBI); ligand shared between two neighboring subunits; in other chain
Binding site236-237ATP (UniProtKB | ChEBI); ligand shared between two neighboring subunits; in other chain
Binding site245ATP (UniProtKB | ChEBI); ligand shared between two neighboring subunits
Binding site245L-methionine (UniProtKB | ChEBI); ligand shared between two neighboring subunits
Binding site251-252ATP (UniProtKB | ChEBI); ligand shared between two neighboring subunits; in other chain
Binding site268ATP (UniProtKB | ChEBI); ligand shared between two neighboring subunits
Binding site272ATP (UniProtKB | ChEBI); ligand shared between two neighboring subunits
Binding site276L-methionine (UniProtKB | ChEBI); ligand shared between two neighboring subunits; in other chain

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular FunctionATP binding
Molecular Functionmagnesium ion binding
Molecular Functionmethionine adenosyltransferase activity
Biological Processone-carbon metabolic process
Biological ProcessS-adenosylmethionine biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    S-adenosylmethionine synthase
  • EC number
  • Short names
    AdoMet synthase
  • Alternative names
    • MAT
    • Methionine adenosyltransferase

Gene names

    • Name
      metK
    • Ordered locus names
      DVU_2449

Organism names

Accessions

  • Primary accession
    Q729A3

Proteomes

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00001745171-391S-adenosylmethionine synthase

Proteomic databases

Interaction

Subunit

Homotetramer; dimer of dimers.

Binary interactions

TypeEntry 1Entry 2Number of experimentsIntact
BINARY Q729A3DVU_0493 Q72ES62EBI-10071362, EBI-10071066

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for region.

TypeIDPosition(s)Description
Region103-113Flexible loop

Sequence similarities

Belongs to the AdoMet synthase family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    391
  • Mass (Da)
    42,424
  • Last updated
    2004-07-05 v1
  • Checksum
    89E6CF9683547FFD
MIPSKGKYYFTSESVTEGHPDKVADQISDAVLDVLLAQDPNSRVACETLVTTGMAVIAGEITTRGYADLPHVVRETIRNIGYNSSEMGFDWQTCAVISSIDKQSADIAQGVDRATNEDQGAGDQGMMFGFACDETATLMPAPIYWAHQLSQRLTEVRKDGTVDIFRPDGKTQVSFEYVDGKPVRINNVVVSTQHKDSASQADIIDAVKTHVIRPILEPSGFFDEKACDIFINTTGRFVIGGPMGDCGLTGRKIIQDTYGGMGHHGGGAFSGKDASKVDRSGAYMARYIAKNVVASGLAPKCEVQIAYCIGVAEPVSVLVSSQGTASVPDEVLTRAVREVFDLRPFHITRRLDLLRPIYGKTSCYGHFGRELPEFTWEHTDAAADLRTAAKV

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AE017285
EMBL· GenBank· DDBJ
AAS96921.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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