Q727C6 · RPOC_NITV2
- ProteinDNA-directed RNA polymerase subunit beta'
- GenerpoC
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids1385 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score3/5
Function
function
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.
Catalytic activity
- a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)
Cofactor
Protein has several cofactor binding sites:
Note: Binds 1 Mg2+ ion per subunit.
Note: Binds 2 Zn2+ ions per subunit.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 75 | Zn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 77 | Zn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 90 | Zn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 93 | Zn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 466 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 468 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 470 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 809 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 883 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 890 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 893 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: C |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | DNA-directed RNA polymerase complex | |
Molecular Function | DNA binding | |
Molecular Function | DNA-directed 5'-3' RNA polymerase activity | |
Molecular Function | magnesium ion binding | |
Molecular Function | zinc ion binding | |
Biological Process | DNA-templated transcription |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDNA-directed RNA polymerase subunit beta'
- EC number
- Short namesRNAP subunit beta'
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Thermodesulfobacteriota > Desulfovibrionia > Desulfovibrionales > Desulfovibrionaceae > Nitratidesulfovibrio
Accessions
- Primary accessionQ727C6
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000225532 | 1-1385 | DNA-directed RNA polymerase subunit beta' | |||
Sequence: MTLDDLFTVRGSAANIANIRNLKAIQITIASPENIREWSYGEVKKPETINYRTFKPERDGLFCAKIFGPVKDYECNCGKYKRMKHRGIVCEKCGVEVIASKVRRERMGHIELAAPVAHIWFLKTLPSKIGTLLDMTMADLEKVLYFDSYIVLDPGSTNLTKMQVISEDQYLQVIDHYGEDALTVGMGAEAVRSLLEELNLEELRVQLREESQATKSQTKKKKLTKRLKIVEAFLESNNKPEWMVMEVIPVIPPELRPLVPLDGGRFATSDLNDLYRRVINRNNRLKRLMELGAPDIIIRNEKRMLQEAVDALFDNGRRGRAITGTNGRPLKSLSDMIKGKQGRFRQNLLGKRVDYSGRSVIVVGPKLKLHQCGLPKKMALELFKPFIYSELEKRGLASTIKSAKKMVEREELVVWDILEEVVREYPIMLNRAPTLHRLGIQSFEPLLVEGKAIQLHPLVCSAYNADFDGDQMAVHVPLSVEAQIECRVLMMSTNNILSPANGSPVIVPSQDIVLGLYYMTVDRSFEKGENMSFCAPWEVVAAYDAGVVALHARINVRMEDGKVVRTTVGRILVWELLPHCVPFSMVNTTLTKKNIARLVSTAYRDAGTKATVILCDRLKDVGYEYATRAGVTIAVKDLTIPSTKKGLIETAQNEVDDIERQYRDGIITRTEKYNKVVDVWTKATQDVSNEMIREISSDIVEDPRTGAKEANSSFNSIYMMSTSGARGNQDQMRQLAGMRGLMAKPSGEIIETPITSSFREGLSVLQYFTSTHGARKGLADTALKTANSGYLTRRLVDVVQDVIVSEHDCGTVDGIELTHIKEGGEIKIPLADRALGRVLLYPVYDPETRDLLFPENTLVDENVAKVLVEREVSSVMIRSALTCQSDRGICTLCYGRDLARGHIVNIGETVGIIAAQSIGEPGTQLTMRTFHIGGTASREIERSSFEAQHPGRVILSRVKAVRNRDGQYMVMGKSGQLAIVDDQGREREKYTLPNGSRLLVTEGEEIRKGQILAEWDPFNEPFVSEVDGVIRFSDIVEGKTFQEKMDEATRMTTQTIIEYRTTNFRPSISICDEHGEVKMRGNNIPATYSLPVGAIIMVKNGQDLQAGDIIARKPRETSKTKDIVGGLPRVAELFEVRKPKDMAVVSEIAGIVTYAGETKGKRKLVVTPEIGEAKEYLVPKGKHITVTDGDFVEAGDLLTEGHPELHDILRTRGEKYLARYLTDEIQEVYRFQGVAIDDKHIEVIVRQMLKKVTVLDPGGTTFLVGEQVDKGEFRVENTRAMGEGRTPATAEPLVLGITQASLTTSSFISAASFQETTKVLTEASLRGKMDYLRGLKENVIVGRLIPAGTGYREYVNTDILVPEQRERPDKFLEDLEENPLLVDIY |
Proteomic databases
Interaction
Subunit
The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | Q727C6 | rpoB Q727C7 | 4 | EBI-10065699, EBI-10066295 |
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length1,385
- Mass (Da)154,813
- Last updated2004-07-05 v1
- Checksum1B7C3027F1EF7907
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AE017285 EMBL· GenBank· DDBJ | AAS97401.1 EMBL· GenBank· DDBJ | Genomic DNA |