Q70I53 · HDAH_ALCSD
- ProteinHistone deacetylase-like amidohydrolase
- GenehdaH
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids369 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Exhibits significant levels of protein deacetylase activity comparable to those of eukaryotic HDACs in assays both with fluorogenic peptidic substrates and acetate-radiolabeled histones. Accepts proteins with epsilon-acetylated lysine residues and tritiated-acetate-prelabeled chicken histones as substrates. The natural substrate protein is not yet known.
Cofactor
Note: Binds 1 zinc ion per subunit.
Activity regulation
Zinc, and cobalt and nickel at a lesser extent, are able to increase the catalytic activity (2.2-, 1.3- and 1.1-fold respectively) at concentrations of 1 mM. Higher concentrations have an inhibitory effect. Magnesium, manganese and calcium have no effect on activity at concentrations between 0 and 10 mM. At 100 mM, the catalytic activity is increased between 1.2- and 2.1-fold. Hydroxamates like TSA and SAHA inhibit the enzyme (PubMed:15060035).
Is also inhibited by azobenzenes, stilbenes and arylazopyrazoles (PubMed:27756124).
Is also inhibited by azobenzenes, stilbenes and arylazopyrazoles (PubMed:27756124).
pH Dependence
Optimum pH is 8.0.
Features
Showing features for active site, binding site, site.
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | hydrolase activity | |
Molecular Function | metal ion binding |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameHistone deacetylase-like amidohydrolase
- EC number
- Short namesHDAC-like amidohydrolase; HDAH
Gene names
Organism names
- Taxonomic lineageBacteria > Pseudomonadota > Betaproteobacteria > Burkholderiales > Alcaligenaceae
Accessions
- Primary accessionQ70I53
Phenotypes & Variants
Chemistry
PTM/Processing
Features
Showing features for initiator methionine, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Initiator methionine | 1 | Removed | ||||
Sequence: M | ||||||
Chain | PRO_0000114728 | 2-369 | Histone deacetylase-like amidohydrolase | |||
Sequence: AIGYVWNTLYGWVDTGTGSLAAANLTARMQPISHHLAHPDTKRRFHELVCASGQIEHLTPIAAVAATDADILRAHSAAHLENMKRVSNLPTGGDTGDGITMMGNGGLEIARLSAGGAVELTRRVATGELSAGYALVNPPGHHAPHNAAMGFCIFNNTSVAAGYARAVLGMERVAILDWDVHHGNGTQDIWWNDPSVLTISLHQHLCFPPDSGYSTERGAGNGHGYNINVPLPPGSGNAAYLHAMDQVVLHALRAYRPQLIIVGSGFDASMLDPLARMMVTADGFRQMARRTIDCAADICDGRIVFVQEGGYSPHYLPFCGLAVIEELTGVRSLPDPYHEFLAGMGGNTLLDAERAAIEEIVPLLADIR |
Interaction
Structure
Sequence
- Sequence statusComplete
- Length369
- Mass (Da)39,424
- Last updated2007-01-23 v3
- Checksum25A8C71FFF7C9FE7
Keywords
- Technical term