Q6ZQ23 · Q6ZQ23_MOUSE
- ProteinEndoribonuclease Dicer
- GeneDicer1
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids1475 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score3/5
Function
function
Double-stranded RNA (dsRNA) endoribonuclease playing a central role in short dsRNA-mediated post-transcriptional gene silencing. Cleaves naturally occurring long dsRNAs and short hairpin pre-microRNAs (miRNA) into fragments of twenty-one to twenty-three nucleotides with 3' overhang of two nucleotides, producing respectively short interfering RNAs (siRNA) and mature microRNAs. SiRNAs and miRNAs serve as guide to direct the RNA-induced silencing complex (RISC) to complementary RNAs to degrade them or prevent their translation. Gene silencing mediated by siRNAs, also called RNA interference, controls the elimination of transcripts from mobile and repetitive DNA elements of the genome but also the degradation of exogenous RNA of viral origin for instance. The miRNA pathway on the other side is a mean to specifically regulate the expression of target genes.
Catalytic activity
Cofactor
Protein has several cofactor binding sites:
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | ATP binding | |
Molecular Function | helicase activity | |
Molecular Function | ribonuclease III activity | |
Molecular Function | RNA binding | |
Biological Process | pre-miRNA processing | |
Biological Process | rRNA processing | |
Biological Process | siRNA processing |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameEndoribonuclease Dicer
- EC number
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionQ6ZQ23
Organism-specific databases
PTM/Processing
Proteomic databases
Structure
Family & Domains
Features
Showing features for domain, region, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 1-161 | Helicase C-terminal | ||||
Sequence: EPFLKVVLCGEIKLCFLRLIKEAGKQDPELAYISSNFITGHGIGKNQPRSKQMEAEFRKQEEVLRKFRAHETNLLIATSVVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYVMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSADGAEADVH | ||||||
Domain | 189-281 | Dicer dsRNA-binding fold | ||||
Sequence: AIGHINRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMDSVRLAERVVALICCEKLHKIGELDEHLMPVGKETVKY | ||||||
Region | 285-304 | Disordered | ||||
Sequence: LDLHDEEETSVPGRPGSTKR | ||||||
Domain | 454-601 | PAZ | ||||
Sequence: KFMEDIEKSEARIGIPSTKYSKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHP | ||||||
Domain | 842-931 | RNase III | ||||
Sequence: VGYSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVS | ||||||
Region | 1157-1186 | Disordered | ||||
Sequence: ALDPAQENGSSQQKSLSGSCASPVGPRSSA | ||||||
Compositional bias | 1159-1180 | Polar residues | ||||
Sequence: DPAQENGSSQQKSLSGSCASPV | ||||||
Domain | 1219-1377 | RNase III | ||||
Sequence: FETFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVKFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSG | ||||||
Domain | 1402-1467 | DRBM | ||||
Sequence: VPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKA |
Sequence similarities
Belongs to the helicase family. Dicer subfamily.
Family and domain databases
Sequence
- Sequence statusFragment
- Length1,475
- Mass (Da)166,367
- Last updated2004-07-05 v1
- ChecksumF7D14427C50E61F5
Features
Showing features for non-terminal residue, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Non-terminal residue | 1 | |||||
Sequence: E | ||||||
Compositional bias | 1159-1180 | Polar residues | ||||
Sequence: DPAQENGSSQQKSLSGSCASPV |