Q6Z6D6 · C7342_ORYSJ
- ProteinCytochrome P450 734A2
- GeneCYP734A2
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids557 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score4/5
Function
function
Cytochrome P450 involved in brassinosteroids (BRs) inactivation and regulation of BRs homeostasis. Is a multifunctional and multisubstrate enzyme that controls the endogenous bioactive BR content both by direct inactivation of castasterone (CS) and by decreasing the levels of BR precursors. Catalyzes the oxidation of carbon 22 hydroxylated BR intermediates to produce C26 oxidized metabolites.
Miscellaneous
Plants overexpressing CYP734A2 show a dwarf phenotype, characterized by abnormal leaves with stiff, tortuous blades, undeveloped leaf sheaths, no flowering and bear seeds. Over-expression of CYP734A2 causes an important reduction of the levels of the BRs castasterone (CS), 6-deoxocastasterone (6-deoxoCS) and 6-deoxotyphasterol (6-deoxoTY).
Cofactor
Features
Showing features for binding site.
GO annotations
all annotations | all molecular function | nucleotide binding | molecular_function | nucleic acid binding | dna binding | chromatin binding | dna-binding transcription factor activity | rna binding | cytoskeletal motor activity | catalytic activity | nuclease activity | signaling receptor binding | structural molecule activity | transporter activity | binding | protein binding | translation factor activity, rna binding | lipid binding | kinase activity | transferase activity | hydrolase activity | oxygen binding | enzyme regulator activity | carbohydrate binding | signaling receptor activity | translation regulator activity | transcription regulator activity | other molecular function | all biological process | carbohydrate metabolic process | generation of precursor metabolites and energy | nucleobase-containing compound metabolic process | dna metabolic process | translation | lipid metabolic process | transport | response to stress | cell cycle | cell communication | signal transduction | cell-cell signaling | multicellular organism development | circadian rhythm | biological_process | metabolic process | catabolic process | biosynthetic process | response to light stimulus | response to external stimulus | tropism | response to biotic stimulus | response to abiotic stimulus | response to endogenous stimulus | embryo development | post-embryonic development | fruit ripening | abscission | pollination | flower development | cellular process | programmed cell death | photosynthesis | cellular component organization | cell growth | protein metabolic process | cellular homeostasis | secondary metabolic process | reproductive process | cell differentiation | protein modification process | growth | epigenetic regulation of gene expression | response to chemical | anatomical structure development | regulation of molecular function | other biological process | all cellular component | cellular_component | extracellular region | cell wall | intracellular anatomical structure | nucleus | nuclear envelope | nucleoplasm | nucleolus | cytoplasm | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | cytosol | ribosome | cytoskeleton | plasma membrane | chloroplast | plastid | thylakoid | membrane | external encapsulating structure | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | membrane | |
Molecular Function | heme binding | |
Molecular Function | iron ion binding | |
Molecular Function | monooxygenase activity | |
Molecular Function | oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen | |
Biological Process | brassinosteroid homeostasis | |
Biological Process | brassinosteroid metabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameCytochrome P450 734A2
- EC number
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > Liliopsida > Poales > Poaceae > BOP clade > Oryzoideae > Oryzeae > Oryzinae > Oryza > Oryza sativa
Accessions
- Primary accessionQ6Z6D6
- Secondary accessions
Proteomes
Genome annotation databases
Subcellular Location
UniProt Annotation
GO Annotation
Membrane ; Single-pass membrane protein
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transmembrane | 13-35 | Helical | ||||
Sequence: WATWRVAAVAAAAAVWVTMHVAA |
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000411201 | 1-557 | Cytochrome P450 734A2 | |||
Sequence: MEEDGGGGAGWGWATWRVAAVAAAAAVWVTMHVAARMADALWWRPRRLEAHFAAQGVRGPPYRFLLGSVREMVALMAEASSKPMSPPTSHNALPRVLAFYHYWRKIYGHRFLIWFGPTPRLTVAEPELIREIFLTRADAFDRYEAHPVVRQLEGDGLVSLHGDKWALHRRVLTDAFYPDNLNRLIPHVGKSVAALAAKWGAMAEAGGSGEVEVDVAEWFQAVTEEAITRATFGRSYDDGRVVFAMQGQLMAFASEAFRKVLVPGYRFLPTKKNRLSWRLDREIRRSLMRLIGRRSDEAEQGEKADDGSFRDLLGLMINAGAAAATRGNAGGEKNSPAAAIPVEDMLEECKTFFFAGKQTTTNLLTWATVLLAMHPDWQERARREVFDVCGAGELPSKEHLPKLKTLGMIMNETLRLYPPAVATIRRAKVDVQLSDGCMIPRDMELLVPIMAIHHDTRYWGPDASQFNPARFANGASKAAKHPLAFIPFGLGSRMCVGQNLARLEAKLTMAILLQRFEIRTSPNYVHAPTVLMLLYPQYGAPLIFRPLSSHPPDSTGP |
Proteomic databases
Expression
Tissue specificity
Expressed in roots, shoot apex, leaf sheaths and leaf blades.
Induction
By brassinolide (BL) and dark treatment.
Interaction
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length557
- Mass (Da)61,964
- Last updated2004-07-05 v1
- Checksum294621C790EDF9C1
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AB488666 EMBL· GenBank· DDBJ | BAH23799.1 EMBL· GenBank· DDBJ | mRNA | ||
AP005008 EMBL· GenBank· DDBJ | BAD16010.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AP005915 EMBL· GenBank· DDBJ | BAD38430.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AP008208 EMBL· GenBank· DDBJ | BAH91576.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AP014958 EMBL· GenBank· DDBJ | BAS77550.1 EMBL· GenBank· DDBJ | Genomic DNA |