Q6Z6D6 · C7342_ORYSJ

Function

function

Cytochrome P450 involved in brassinosteroids (BRs) inactivation and regulation of BRs homeostasis. Is a multifunctional and multisubstrate enzyme that controls the endogenous bioactive BR content both by direct inactivation of castasterone (CS) and by decreasing the levels of BR precursors. Catalyzes the oxidation of carbon 22 hydroxylated BR intermediates to produce C26 oxidized metabolites.

Miscellaneous

Plants overexpressing CYP734A2 show a dwarf phenotype, characterized by abnormal leaves with stiff, tortuous blades, undeveloped leaf sheaths, no flowering and bear seeds. Over-expression of CYP734A2 causes an important reduction of the levels of the BRs castasterone (CS), 6-deoxocastasterone (6-deoxoCS) and 6-deoxotyphasterol (6-deoxoTY).

Cofactor

heme (UniProtKB | Rhea| CHEBI:30413 )

Features

Showing features for binding site.

155750100150200250300350400450500550
TypeIDPosition(s)Description
Binding site495Fe (UniProtKB | ChEBI) of heme (UniProtKB | ChEBI); axial binding residue

GO annotations

all annotationsall molecular functionnucleotide bindingmolecular_functionnucleic acid bindingdna bindingchromatin bindingdna-binding transcription factor activityrna bindingcytoskeletal motor activitycatalytic activitynuclease activitysignaling receptor bindingstructural molecule activitytransporter activitybindingprotein bindingtranslation factor activity, rna bindinglipid bindingkinase activitytransferase activityhydrolase activityoxygen bindingenzyme regulator activitycarbohydrate bindingsignaling receptor activitytranslation regulator activitytranscription regulator activityother molecular functionall biological processcarbohydrate metabolic processgeneration of precursor metabolites and energynucleobase-containing compound metabolic processdna metabolic processtranslationlipid metabolic processtransportresponse to stresscell cyclecell communicationsignal transductioncell-cell signalingmulticellular organism developmentcircadian rhythmbiological_processmetabolic processcatabolic processbiosynthetic processresponse to light stimulusresponse to external stimulustropismresponse to biotic stimulusresponse to abiotic stimulusresponse to endogenous stimulusembryo developmentpost-embryonic developmentfruit ripeningabscissionpollinationflower developmentcellular processprogrammed cell deathphotosynthesiscellular component organizationcell growthprotein metabolic processcellular homeostasissecondary metabolic processreproductive processcell differentiationprotein modification processgrowthepigenetic regulation of gene expressionresponse to chemicalanatomical structure developmentregulation of molecular functionother biological processall cellular componentcellular_componentextracellular regioncell wallintracellular anatomical structurenucleusnuclear envelopenucleoplasmnucleoluscytoplasmmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuscytosolribosomecytoskeletonplasma membranechloroplastplastidthylakoidmembraneexternal encapsulating structureother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentmembrane
Molecular Functionheme binding
Molecular Functioniron ion binding
Molecular Functionmonooxygenase activity
Molecular Functionoxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
Biological Processbrassinosteroid homeostasis
Biological Processbrassinosteroid metabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

Gene names

    • Name
      CYP734A2
    • ORF names
      OSJNBb0056C19.10, P0544H11.26
    • Ordered locus names
      Os02g0204700, LOC_Os02g11020

Organism names

  • Taxonomic identifier
  • Strain
    • cv. Nipponbare
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > Liliopsida > Poales > Poaceae > BOP clade > Oryzoideae > Oryzeae > Oryzinae > Oryza > Oryza sativa

Accessions

  • Primary accession
    Q6Z6D6
  • Secondary accessions
    • A0A0P0VG55

Proteomes

Genome annotation databases

Subcellular Location

Membrane
; Single-pass membrane protein

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane13-35Helical

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004112011-557Cytochrome P450 734A2

Proteomic databases

Expression

Tissue specificity

Expressed in roots, shoot apex, leaf sheaths and leaf blades.

Induction

By brassinolide (BL) and dark treatment.

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Belongs to the cytochrome P450 family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    557
  • Mass (Da)
    61,964
  • Last updated
    2004-07-05 v1
  • Checksum
    294621C790EDF9C1
MEEDGGGGAGWGWATWRVAAVAAAAAVWVTMHVAARMADALWWRPRRLEAHFAAQGVRGPPYRFLLGSVREMVALMAEASSKPMSPPTSHNALPRVLAFYHYWRKIYGHRFLIWFGPTPRLTVAEPELIREIFLTRADAFDRYEAHPVVRQLEGDGLVSLHGDKWALHRRVLTDAFYPDNLNRLIPHVGKSVAALAAKWGAMAEAGGSGEVEVDVAEWFQAVTEEAITRATFGRSYDDGRVVFAMQGQLMAFASEAFRKVLVPGYRFLPTKKNRLSWRLDREIRRSLMRLIGRRSDEAEQGEKADDGSFRDLLGLMINAGAAAATRGNAGGEKNSPAAAIPVEDMLEECKTFFFAGKQTTTNLLTWATVLLAMHPDWQERARREVFDVCGAGELPSKEHLPKLKTLGMIMNETLRLYPPAVATIRRAKVDVQLSDGCMIPRDMELLVPIMAIHHDTRYWGPDASQFNPARFANGASKAAKHPLAFIPFGLGSRMCVGQNLARLEAKLTMAILLQRFEIRTSPNYVHAPTVLMLLYPQYGAPLIFRPLSSHPPDSTGP

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AB488666
EMBL· GenBank· DDBJ
BAH23799.1
EMBL· GenBank· DDBJ
mRNA
AP005008
EMBL· GenBank· DDBJ
BAD16010.1
EMBL· GenBank· DDBJ
Genomic DNA
AP005915
EMBL· GenBank· DDBJ
BAD38430.1
EMBL· GenBank· DDBJ
Genomic DNA
AP008208
EMBL· GenBank· DDBJ
BAH91576.1
EMBL· GenBank· DDBJ
Genomic DNA
AP014958
EMBL· GenBank· DDBJ
BAS77550.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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