Q6YPZ6 · TSAD_ONYPE
- ProteintRNA N6-adenosine threonylcarbamoyltransferase
- GenetsaD
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids233 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaE and TsaB. TsaD likely plays a direct catalytic role in this reaction (By similarity).
Catalytic activity
- L-threonylcarbamoyladenylate + adenosine37 in tRNA = N6-L-threonylcarbamoyladenosine37 in tRNA + AMP + H+
Cofactor
Note: Binds 1 Fe2+ ion per subunit.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 111 | Fe cation (UniProtKB | ChEBI) | |||
Binding site | 115 | Fe cation (UniProtKB | ChEBI) | |||
Binding site | 133-137 | substrate | |||
Binding site | 166 | substrate | |||
Binding site | 179 | substrate | |||
Binding site | 183 | substrate | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | metal ion binding | |
Molecular Function | N(6)-L-threonylcarbamoyladenine synthase activity | |
Biological Process | tRNA threonylcarbamoyladenosine modification |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nametRNA N6-adenosine threonylcarbamoyltransferase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Mycoplasmatota > Mollicutes > Acholeplasmatales > Acholeplasmataceae > Candidatus Phytoplasma > 16SrI (Aster yellows group)
Accessions
- Primary accessionQ6YPZ6
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000303464 | 1-233 | tRNA N6-adenosine threonylcarbamoyltransferase | ||
Interaction
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length233
- Mass (Da)25,543
- Last updated2004-07-05 v1
- ChecksumDEB2A7AF0E3582B1
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AP006628 EMBL· GenBank· DDBJ | BAD04664.1 EMBL· GenBank· DDBJ | Genomic DNA |