Q6Y4S4 · KISS1_MOUSE
- ProteinMetastasis-suppressor KiSS-1
- GeneKiss1
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids130 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Metastasis suppressor protein. May regulate events downstream of cell-matrix adhesion, perhaps involving cytoskeletal reorganization. Generates a C-terminally amidated peptide, metastin which functions as the endogenous ligand of the G-protein coupled receptor GPR54. Activation of the receptor inhibits cell proliferation and cell migration, key characteristics of tumor metastasis. The receptor is also essential for normal gonadotropin-released hormone physiology and for puberty. The hypothalamic KiSS1/GPR54 system is a pivotal factor in central regulation of the gonadotropic axis at puberty and in adulthood. Intracerebroventricular administration induces an increase in serum LH and FSH levels in prepubertal male and female as well as in adult animals.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | apical plasma membrane | |
Cellular Component | extracellular space | |
Cellular Component | neuronal cell body | |
Molecular Function | kisspeptin receptor binding | |
Biological Process | decidualization | |
Biological Process | G protein-coupled receptor signaling pathway | |
Biological Process | generation of ovulation cycle rhythm | |
Biological Process | negative regulation of cell population proliferation | |
Biological Process | phospholipase C-activating G protein-coupled receptor signaling pathway | |
Biological Process | positive regulation of cytosolic calcium ion concentration | |
Biological Process | positive regulation of growth hormone secretion | |
Biological Process | positive regulation of luteinizing hormone secretion | |
Biological Process | positive regulation of MAPK cascade | |
Biological Process | positive regulation of synaptic transmission |
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameMetastasis-suppressor KiSS-1
- Alternative names
- Cleaved into 2 chains
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionQ6Y4S4
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
Phenotypes & Variants
Disruption phenotype
Animals are viable and healthy with no apparent abnormalities but fail to undergo sexual maturation. Mutant female do not progress through the estrous cycle, have thread-like uteri and small ovaries, and do not produce mature Graffian follicles. Mutant males have small testes, and spermatogenesis arrested mainly at the early haploid spermatid stage. Both sexes have low circulating gonadotropin (LH and FSH) and sex steroid (beta-estradiol or testosterone) hormone levels. Migration of GnRH neurons into the hypothalamus appears normal with appropriate axonal connections to the median eminence and total GnRH content. The hypothalamic-pituitary axis is functional, as shown by robust LH secretion after peripheral administration of kisspeptin.
PTM/Processing
Features
Showing features for signal, chain, peptide, disulfide bond, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Signal | 1-19 | |||||
Sequence: MISMASWQLLLLLCVATYG | ||||||
Chain | PRO_0000021552 | 20-130 | Metastasis-suppressor KiSS-1 | |||
Sequence: EPLAKVAPLVKPGSTGQQSGPQELVNAWEKESRYAESKPGSAGLRARRSSPCPPVEGPAGRQRPLCASRSRLIPAPRGAVLVQREKDLSTYNWNSFGLRYGRRQAARAARG | ||||||
Peptide | PRO_0000021553 | 68-119 | Metastin | |||
Sequence: SSPCPPVEGPAGRQRPLCASRSRLIPAPRGAVLVQREKDLSTYNWNSFGLRY | ||||||
Disulfide bond | 71↔85 | |||||
Sequence: CPPVEGPAGRQRPLC | ||||||
Modified residue | 110 | Phosphotyrosine | ||||
Sequence: Y | ||||||
Peptide | PRO_0000021554 | 110-119 | Kisspeptin-10 | |||
Sequence: YNWNSFGLRY | ||||||
Modified residue | 119 | Tyrosine amide | ||||
Sequence: Y |
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
Weak in all tissue types with highest levels in lung and 15- 17-day embryos. Expressed in areas of the hypothalamus implicated in the neuroendocrine regulation of gonadotropin secretion, including the anteroventral periventricular nucleus, the periventricular nucleus, and the arcuate nucleus.
Gene expression databases
Structure
Family & Domains
Features
Showing features for region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 49-82 | Disordered | ||||
Sequence: KESRYAESKPGSAGLRARRSSPCPPVEGPAGRQR | ||||||
Region | 110-119 | Essential for receptor binding and receptor activation | ||||
Sequence: YNWNSFGLRY |
Sequence similarities
Belongs to the KISS1 family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence & Isoform
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
This entry describes 2 isoforms produced by Alternative splicing.
Q6Y4S4-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- Length130
- Mass (Da)14,117
- Last updated2005-03-01 v2
- ChecksumAC9D30CF84B4F41B
Q6Y4S4-2
- Name2
- Differences from canonical
- 25-28: Missing
Sequence caution
Features
Showing features for alternative sequence.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Alternative sequence | VSP_012957 | 25-28 | in isoform 2 | |||
Sequence: Missing |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF472576 EMBL· GenBank· DDBJ | AAP13356.1 EMBL· GenBank· DDBJ | mRNA | ||
AY182231 EMBL· GenBank· DDBJ | AAO64981.1 EMBL· GenBank· DDBJ | mRNA | Different initiation | |
AB162440 EMBL· GenBank· DDBJ | BAD36756.1 EMBL· GenBank· DDBJ | mRNA | ||
AY707858 EMBL· GenBank· DDBJ | AAW34131.1 EMBL· GenBank· DDBJ | mRNA | ||
BC117046 EMBL· GenBank· DDBJ | AAI17047.1 EMBL· GenBank· DDBJ | mRNA | ||
BC119348 EMBL· GenBank· DDBJ | AAI19349.1 EMBL· GenBank· DDBJ | mRNA |