Q6XTB6 · PA_I69A0

Function

function

Plays an essential role in viral RNA transcription and replication by forming the heterotrimeric polymerase complex together with PB1 and PB2 subunits. The complex transcribes viral mRNAs by using a unique mechanism called cap-snatching. It consists in the hijacking and cleavage of host capped pre-mRNAs. These short capped RNAs are then used as primers for viral mRNAs. The PB2 subunit is responsible for the binding of the 5' cap of cellular pre-mRNAs which are subsequently cleaved after 10-13 nucleotides by the PA subunit that carries the endonuclease activity.

Cofactor

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Note: Binds 2 manganese ions per subunit.

Features

Showing features for binding site.

1716100200300400500600700
TypeIDPosition(s)Description
Binding site41Mn2+ 1 (UniProtKB | ChEBI)
Binding site80Mn2+ 2 (UniProtKB | ChEBI)
Binding site108Mn2+ 1 (UniProtKB | ChEBI)
Binding site108Mn2+ 2 (UniProtKB | ChEBI)
Binding site119Mn2+ 1 (UniProtKB | ChEBI)
Binding site120Mn2+ 1 (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componenthost cell cytoplasm
Cellular Componenthost cell nucleus
Molecular Functionendonuclease activity
Molecular Functionmetal ion binding
Molecular FunctionRNA binding
Biological Processcap snatching
Biological ProcessDNA-templated transcription
Biological Processsymbiont-mediated suppression of host mRNA transcription via inhibition of RNA polymerase II activity
Biological Processviral RNA genome replication
Biological Processviral translational frameshifting

Keywords

Enzyme and pathway databases

Protein family/group databases

Names & Taxonomy

Protein names

  • Recommended name
    Polymerase acidic protein
  • EC number
  • Alternative names
    • RNA-directed RNA polymerase subunit P2

Gene names

    • Name
      PA

Organism names

Accessions

  • Primary accession
    Q6XTB6

Subcellular Location

Host cytoplasm
Host nucleus
Note: PB1 and PA are transported in the host nucleus as a complex.

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00002792451-716Polymerase acidic protein

Post-translational modification

Phosphorylated on serines and threonines by host kinases, including human casein kinase II.

Keywords

Interaction

Subunit

Influenza RNA polymerase is composed of three subunits: PB1, PB2 and PA. Interacts (via C-terminus) with PB1 (via N-terminus).

Structure

3D structure databases

Family & Domains

Features

Showing features for motif.

TypeIDPosition(s)Description
Motif124-139Nuclear localization signal 1 (NLS1)
Motif184-247Nuclear localization signal 2 (NLS2)

Sequence similarities

Belongs to the influenza viruses PA family.

Family and domain databases

Sequence & Isoform

Align isoforms (2)
  • Sequence status
    Complete

This entry describes 2 isoforms produced by Ribosomal frameshifting.

Q6XTB6-1

This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

  • Length
    716
  • Mass (Da)
    82,932
  • Last updated
    2004-07-05 v1
  • Checksum
    166BB6B11A5CE172
MEDFVRQCFNPMIVELAEKAMKEYGEDLKIETNKFAAICTHLEVCFMYSDFHFINEQGESIVVELDDPNALLKHRFEIIEGRDRTMAWTVVNSICNTTGAEKPKFLPDLYDYKENRFIEIGVTRREVHIYYLEKANKIKSENTHIHIFSFTGEEMATKADYTLDEESRARIKTRLFTIRQEMANRGLWDSFRQSERGEETIEERFEITGTMRRLADQSLPPNFSCLENFRAYVDGFEPNGCIEGKLSQMSKEVNARIEPFLKTTPRPIRLPDGPPCFQRSKFLLMDALKLSIEDPSHEGEGIPLYDAIKCMRTFFGWKEPYIVKPHEKGINPNYLLSWKQVLAELQDIENEEKIPRTKNMKKTSQLKWALGENMAPEKVDFDNCRDISDLKQYDSDEPELRSLSSWIQNEFNKACELTDSIWIELDEIGEDVAPIEYIASMRRNYFTAEVSHCRATEYIMKGVYINTALLNASCAAMDDFQLIPMISKCRTKEGRRKTNLYGFIIKGRSHLRNDTDVVNFVSMEFSLTDPRLEPHKWEKYCVLEIGDMILRSAIGQMSRPMFLYVRTNGTSKIKMKWGMEMRRCLLQSLQQIESMIEAESSVKEKDMTKEFFENKSETWPIGESPKGVEEGSIGKVCRTLLAKSVFNSLYASPQLEGFSAESRKLLLVVQALRDNLEPGTFDLGGLYEAIEECLINDPWVLLNASWFNSFLTHALR

P0CK85-1

The sequence of this isoform can be found in the external entry linked below. Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

View isoform
  • Name
    PA-X
  • See also
    sequence in UniParc or sequence clusters in UniRef

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AY210196
EMBL· GenBank· DDBJ
AAO46510.1
EMBL· GenBank· DDBJ
Genomic RNA

Similar Proteins

Disclaimer

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