Q6WP49 · BOT1_BOTFU
- ProteinCytochrome P450 monooxygenase BOT1
- GeneBOT1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids510 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Cytochrome P450 monooxygenase; part of the gene cluster that mediates the biosynthesis of botrydial (PubMed:14651630, PubMed:15986930).
Botrydial is necessary for colonization of plant tissue by the T4 strain (PubMed:19035644).
It is a strain-dependent virulence factor since highly aggressive strains like SAS56 or B05 still retain substantial virulence when botrydial synthesis is impaired, since they produce also botcinic acid (PubMed:15986930).
The first step of botrydial biosynthesis is performed by the sesquiterpene synthase BOT2 which catalyzes the cyclization of farnesyl diphosphate (FPP) to presilphiperfolan-8-beta-ol (PSP) (PubMed:19035644, PubMed:19476353).
The cytochrome P450 monooxygenase BOT4 then catalyzes the hydroxylation at C-4 to give a probotryane intermediate (PubMed:27529428, PubMed:28617493).
Acetylation of the hydroxyl at C-4 is carried out by the acetyltransferase BOT5, followed by the combined action of the P450 monooxygenases BOT3 and BOT1, to yield finally the glycol, via the regio- and stereospecific hydroxylations at C-10 and C-15 of the probotryane intermediates, respectively (PubMed:15986930, PubMed:27529428).
The cleavage of the C10-C15 bond of probotryane skeleton is an intriguing and chemically important reaction, which could be mediated by some of the monooxygenases or by a combination of them (PubMed:27529428).
It is possible that either BOT3 or BOT1 would oxidize either the 10- or the 15-hydroxy group to the hydroperoxide derivative, which would then undergo heterolytic fragmentation to give the dialdehyde botrydial (PubMed:27529428).
Finally, the dehydrogenase BOT7 might be involved in the conversion of botrydial to dihydrobotrydial (PubMed:27721016).
Botrydial is necessary for colonization of plant tissue by the T4 strain (PubMed:19035644).
It is a strain-dependent virulence factor since highly aggressive strains like SAS56 or B05 still retain substantial virulence when botrydial synthesis is impaired, since they produce also botcinic acid (PubMed:15986930).
The first step of botrydial biosynthesis is performed by the sesquiterpene synthase BOT2 which catalyzes the cyclization of farnesyl diphosphate (FPP) to presilphiperfolan-8-beta-ol (PSP) (PubMed:19035644, PubMed:19476353).
The cytochrome P450 monooxygenase BOT4 then catalyzes the hydroxylation at C-4 to give a probotryane intermediate (PubMed:27529428, PubMed:28617493).
Acetylation of the hydroxyl at C-4 is carried out by the acetyltransferase BOT5, followed by the combined action of the P450 monooxygenases BOT3 and BOT1, to yield finally the glycol, via the regio- and stereospecific hydroxylations at C-10 and C-15 of the probotryane intermediates, respectively (PubMed:15986930, PubMed:27529428).
The cleavage of the C10-C15 bond of probotryane skeleton is an intriguing and chemically important reaction, which could be mediated by some of the monooxygenases or by a combination of them (PubMed:27529428).
It is possible that either BOT3 or BOT1 would oxidize either the 10- or the 15-hydroxy group to the hydroperoxide derivative, which would then undergo heterolytic fragmentation to give the dialdehyde botrydial (PubMed:27529428).
Finally, the dehydrogenase BOT7 might be involved in the conversion of botrydial to dihydrobotrydial (PubMed:27721016).
Cofactor
Pathway
Secondary metabolite biosynthesis.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | membrane | |
Molecular Function | heme binding | |
Molecular Function | iron ion binding | |
Molecular Function | monooxygenase activity | |
Molecular Function | oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameCytochrome P450 monooxygenase BOT1
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Pezizomycotina > Leotiomycetes > Helotiales > Sclerotiniaceae > Botrytis
Accessions
- Primary accessionQ6WP49
Subcellular Location
UniProt Annotation
GO Annotation
Membrane ; Single-pass membrane protein
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transmembrane | 16-36 | Helical | ||||
Sequence: PLAWAALILASFTLYSVQLVV |
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain, glycosylation.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000444642 | 1-510 | Cytochrome P450 monooxygenase BOT1 | |||
Sequence: MGLLSDVINNFLPETPLAWAALILASFTLYSVQLVVRRLYFHPLSKIPGPFLARSRYWYEFYQDIILGGIYVKNYAALHEKYGPVLRASPDRVHVSDPDFFHEVYSSGSKYMKDPAFFQAAGGIPEALPAIVDVEYHRRRRKLINDLFSAKSMEALSHLVLKVVQNALSKAHEHHEANKVLDIQRLYTGITIDTIMQVLCDRTLNFIDAKEEEEPPFLATLRTFSENFFLLKHFPILIWMALNIPKSIAQKLIPGEFEFRASINQWIRDRASEHELGVEKAEDGRKTVIDLLLRPEDGGRPLTHQAVEDETYSFAFAGTHTTSHTMSMGTYYLLSHPAKLQKLRDELKPIPKNDQGLYEYKTVRSLPYLNACIKESLRMSSPVPGILPRLVPAEGMTWRGHYLPPGTSVSSSIYSVHTDPNIFPNPEQFIPERWLANENLDHYLVVFGKGSRACIGLNVAWMETYLTFSNFFTSLNMTLFETNEQSTDWTDCGNAMIKKHVRVKVDSLAS | ||||||
Glycosylation | 476 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N |
Keywords
- PTM
PTM databases
Expression
Induction
The botrydial biosynthesis cluster genes are co-regulated by the Ca2+/calcineurin signal transduction pathway, which is under the control of the alpha subunit BCG1 of a heterotrimeric G protein (PubMed:14651630, PubMed:19035644).
Expression of the cluster is also positively regulated by the cluster-specific transcription factor BOT6 (PubMed:27721016).
Expression of the cluster is also positively regulated by the cluster-specific transcription factor BOT6 (PubMed:27721016).
Structure
Sequence
- Sequence statusComplete
- Length510
- Mass (Da)58,162
- Last updated2004-07-05 v1
- ChecksumA591F786FC5D565C