Q6TU48 · MAOX_DICDI
- ProteinNADP-dependent malic enzyme
- GenemalA
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids544 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score4/5
Function
Catalytic activity
- (S)-malate + NADP+ = pyruvate + CO2 + NADPH
Cofactor
Mn2+ (UniProtKB | Rhea| CHEBI:29035 )
Note: Divalent metal cations. Prefers magnesium or manganese.
Features
Showing features for active site, binding site, site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Active site | 92 | Proton donor | |||
Binding site | 145 | NAD+ (UniProtKB | ChEBI) | |||
Active site | 163 | Proton acceptor | |||
Binding site | 234 | a divalent metal cation (UniProtKB | ChEBI) | |||
Binding site | 235 | a divalent metal cation (UniProtKB | ChEBI) | |||
Binding site | 258 | a divalent metal cation (UniProtKB | ChEBI) | |||
Binding site | 258 | NAD+ (UniProtKB | ChEBI) | |||
Site | 258 | Important for activity | |||
Binding site | 287-303 | NADP+ (UniProtKB | ChEBI) | |||
Binding site | 400 | NAD+ (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | malate dehydrogenase (decarboxylating) (NAD+) activity | |
Molecular Function | malate dehydrogenase (decarboxylating) (NADP+) activity | |
Molecular Function | malic enzyme activity | |
Molecular Function | metal ion binding | |
Molecular Function | NAD binding | |
Molecular Function | oxaloacetate decarboxylase activity | |
Biological Process | malate metabolic process | |
Biological Process | pyruvate metabolic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameNADP-dependent malic enzyme
- EC number
- Short namesNADP-ME
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Amoebozoa > Evosea > Eumycetozoa > Dictyostelia > Dictyosteliales > Dictyosteliaceae > Dictyostelium
Accessions
- Primary accessionQ6TU48
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000328379 | 1-544 | NADP-dependent malic enzyme | ||
Proteomic databases
Expression
Tissue specificity
Expressed in the fruiting body.
Induction
By cAMP and the MADS-box protein srfA.
Developmental stage
Expressed at late stage of development (20 to 24 hours).
Interaction
Structure
Family & Domains
Features
Showing features for compositional bias, region.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Compositional bias | 1-19 | Polar residues | |||
Region | 1-22 | Disordered | |||
Sequence similarities
Belongs to the malic enzymes family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length544
- Mass (Da)60,652
- Last updated2004-07-05 v1
- MD5 ChecksumE19350ADDB1399B18DFB8A5A11003627
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Compositional bias | 1-19 | Polar residues | |||
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AY387644 EMBL· GenBank· DDBJ | AAQ95658.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAFI02000008 EMBL· GenBank· DDBJ | EAL71186.1 EMBL· GenBank· DDBJ | Genomic DNA |