Q6RYA0 · SABP2_TOBAC

Function

function

Required to convert methyl salicylate (MeSA) to salicylic acid (SA) as part of the signal transduction pathways that activate systemic acquired resistance in systemic tissue. MeSA is believed to be an inactive form that needs to be demethylated to exert a biological effect. Also able to catalyze the conversion of acibenzolar-S-methyl into acibenzolar to induce systemic acquired resistance.

Miscellaneous

Two amino acid substitutions near the catalytic pocket are sufficient to switch from an esterase to a hydroxynitrile lyase activity.

Catalytic activity

Activity regulation

Esterase activity is down-regulated by salicylic acid (SA) or by tetraFA, a synthetic SA analog.

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
8.6 μMmethyl salicylate (MeSA)
kcat is 0.45 sec-1 with methyl salicylate as substrate.

Pathway

Plant hormone biosynthesis.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site13salicylate (UniProtKB | ChEBI)
Active site81Acyl-ester intermediate
Binding site81salicylate (UniProtKB | ChEBI)
Binding site159salicylate (UniProtKB | ChEBI)
Active site210Charge relay system
Active site238Charge relay system
Binding site238salicylate (UniProtKB | ChEBI)
Binding site253salicylate (UniProtKB | ChEBI)
Binding site257salicylate (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular Functionlipase activity
Molecular Functionmethyl indole-3-acetate esterase activity
Molecular Functionmethyl jasmonate esterase activity
Molecular Functionmethyl salicylate esterase activity
Biological Processinnate immune response
Biological Processjasmonic acid metabolic process
Biological Processsalicylic acid metabolic process
Biological Processsystemic acquired resistance, salicylic acid mediated signaling pathway

Keywords

Enzyme and pathway databases

Protein family/group databases

Names & Taxonomy

Protein names

  • Recommended name
    Salicylic acid-binding protein 2
  • EC number
  • Short names
    NtSABP2
  • Alternative names
    • Methyl salicylate esterase

Gene names

    • Name
      SABP2
    • Ordered locus names
      LOC107791137

Organism names

  • Taxonomic identifier
  • Strains
    • cv. Xanthi NC
    • cv. TN90
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > asterids > lamiids > Solanales > Solanaceae > Nicotianoideae > Nicotianeae > Nicotiana

Accessions

  • Primary accession
    Q6RYA0
  • Secondary accessions
    • A0A1S3ZW27

Proteomes

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis12Abolishes methyl salicylate esterase activity and favors hydroxynitrile lyase activity; in association with K-239.
Mutagenesis13Abolishes salicylic acid-binding.
Mutagenesis81Abolishes methyl salicylate esterase activity.
Mutagenesis238Abolishes salicylic acid-binding and methyl salicylate esterase activity.
Mutagenesis239Abolishes methyl salicylate esterase activity and favors hydroxynitrile lyase activity; in association with T-12.

Chemistry

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004181751-260Salicylic acid-binding protein 2

Proteomic databases

Interaction

Protein-protein interaction databases

Family & Domains

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    260
  • Mass (Da)
    29,304
  • Last updated
    2004-07-05 v1
  • Checksum
    9AA49E6423361145
MKEGKHFVLVHGACHGGWSWYKLKPLLEAAGHKVTALDLAASGTDLRKIEELRTLYDYTLPLMELMESLSADEKVILVGHSLGGMNLGLAMEKYPQKIYAAVFLAAFMPDSVHNSSFVLEQYNERTPAENWLDTQFLPYGSPEEPLTSMFFGPKFLAHKLYQLCSPEDLALASSLVRPSSLFMEDLSKAKYFTDERFGSVKRVYIVCTEDKGIPEEFQRWQIDNIGVTEAIEIKGADHMAMLCEPQKLCASLLEIAHKYN

Computationally mapped potential isoform sequences

There is 1 potential isoform mapped to this entry

View all
EntryEntry nameGene nameLength
A0A8M5WKV0A0A8M5WKV0_TOBACLOC107791137260

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AY485932
EMBL· GenBank· DDBJ
AAR87711.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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