Q6RYA0 · SABP2_TOBAC
- ProteinSalicylic acid-binding protein 2
- GeneSABP2
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids260 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Required to convert methyl salicylate (MeSA) to salicylic acid (SA) as part of the signal transduction pathways that activate systemic acquired resistance in systemic tissue. MeSA is believed to be an inactive form that needs to be demethylated to exert a biological effect. Also able to catalyze the conversion of acibenzolar-S-methyl into acibenzolar to induce systemic acquired resistance.
Miscellaneous
Two amino acid substitutions near the catalytic pocket are sufficient to switch from an esterase to a hydroxynitrile lyase activity.
Catalytic activity
- H2O + methyl salicylate = H+ + methanol + salicylateThis reaction proceeds in the forward direction.
Activity regulation
Esterase activity is down-regulated by salicylic acid (SA) or by tetraFA, a synthetic SA analog.
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
8.6 μM | methyl salicylate (MeSA) |
kcat is 0.45 sec-1 with methyl salicylate as substrate.
Pathway
Plant hormone biosynthesis.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 13 | salicylate (UniProtKB | ChEBI) | ||||
Sequence: A | ||||||
Active site | 81 | Acyl-ester intermediate | ||||
Sequence: S | ||||||
Binding site | 81 | salicylate (UniProtKB | ChEBI) | ||||
Sequence: S | ||||||
Binding site | 159 | salicylate (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Active site | 210 | Charge relay system | ||||
Sequence: D | ||||||
Active site | 238 | Charge relay system | ||||
Sequence: H | ||||||
Binding site | 238 | salicylate (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 253 | salicylate (UniProtKB | ChEBI) | ||||
Sequence: L | ||||||
Binding site | 257 | salicylate (UniProtKB | ChEBI) | ||||
Sequence: H |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | lipase activity | |
Molecular Function | methyl indole-3-acetate esterase activity | |
Molecular Function | methyl jasmonate esterase activity | |
Molecular Function | methyl salicylate esterase activity | |
Biological Process | innate immune response | |
Biological Process | jasmonic acid metabolic process | |
Biological Process | salicylic acid metabolic process | |
Biological Process | systemic acquired resistance, salicylic acid mediated signaling pathway |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameSalicylic acid-binding protein 2
- EC number
- Short namesNtSABP2
- Alternative names
Gene names
Organism names
- Strains
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > asterids > lamiids > Solanales > Solanaceae > Nicotianoideae > Nicotianeae > Nicotiana
Accessions
- Primary accessionQ6RYA0
- Secondary accessions
Proteomes
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 12 | Abolishes methyl salicylate esterase activity and favors hydroxynitrile lyase activity; in association with K-239. | ||||
Sequence: G → T | ||||||
Mutagenesis | 13 | Abolishes salicylic acid-binding. | ||||
Sequence: A → L | ||||||
Mutagenesis | 81 | Abolishes methyl salicylate esterase activity. | ||||
Sequence: S → A | ||||||
Mutagenesis | 238 | Abolishes salicylic acid-binding and methyl salicylate esterase activity. | ||||
Sequence: H → A | ||||||
Mutagenesis | 239 | Abolishes methyl salicylate esterase activity and favors hydroxynitrile lyase activity; in association with T-12. | ||||
Sequence: M → K |
Chemistry
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000418175 | 1-260 | Salicylic acid-binding protein 2 | |||
Sequence: MKEGKHFVLVHGACHGGWSWYKLKPLLEAAGHKVTALDLAASGTDLRKIEELRTLYDYTLPLMELMESLSADEKVILVGHSLGGMNLGLAMEKYPQKIYAAVFLAAFMPDSVHNSSFVLEQYNERTPAENWLDTQFLPYGSPEEPLTSMFFGPKFLAHKLYQLCSPEDLALASSLVRPSSLFMEDLSKAKYFTDERFGSVKRVYIVCTEDKGIPEEFQRWQIDNIGVTEAIEIKGADHMAMLCEPQKLCASLLEIAHKYN |
Proteomic databases
Interaction
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length260
- Mass (Da)29,304
- Last updated2004-07-05 v1
- Checksum9AA49E6423361145
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A8M5WKV0 | A0A8M5WKV0_TOBAC | LOC107791137 | 260 |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AY485932 EMBL· GenBank· DDBJ | AAR87711.1 EMBL· GenBank· DDBJ | mRNA |