Q6QCM2 · Q6QCM2_HHV3

Function

function

Assemblin: Protease that plays an essential role in virion assembly within the nucleus. Catalyzes the cleavage of the assembly protein after formation of the spherical procapsid. By that cleavage, the capsid matures and gains its icosahedral shape. The cleavage sites seem to include -Ala-Ser-, -Ala-Ala-, as well as Ala-Thr bonds. Assemblin and cleavages products are evicted from the capsid before or during DNA packaging.
Assembly protein: Plays a major role in capsid assembly. Acts as a scaffold protein by binding major capsid protein. Multimerizes in the nucleus such as major capsid protein forms the icosahedral T=16 capsid. Cleaved by assemblin after capsid completion. The cleavages products are evicted from the capsid before or during DNA packaging.
Capsid scaffolding protein: Acts as a scaffold protein by binding major capsid protein in the cytoplasm, inducing the nuclear localization of both proteins. Multimerizes in the nucleus such as major capsid protein forms the icosahedral T=16 capsid. Autocatalytic cleavage releases the assembly protein, and subsequently abolishes interaction with major capsid protein. Cleavages products are evicted from the capsid before or during DNA packaging.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

  • Cleaves -Ala-|-Ser- and -Ala-|-Ala- bonds in the scaffold protein.
    EC:3.4.21.97 (UniProtKB | ENZYME | Rhea)

Features

Showing features for active site, site.

Type
IDPosition(s)Description
Active site52Charge relay system
Active site120Charge relay system
Active site139Charge relay system
Site236-237Cleavage; by assemblin; Release site

GO annotations

AspectTerm
Cellular Componenthost cell cytoplasm
Cellular Componenthost cell nucleus
Molecular Functionidentical protein binding
Molecular Functionserine-type endopeptidase activity
Biological Processnuclear capsid assembly
Biological Processproteolysis
Biological Processviral release from host cell

Keywords

Protein family/group databases

Names & Taxonomy

Protein names

  • Recommended name
    Capsid scaffolding protein
  • Alternative names
    • Protease precursor
      (pPR
      )
  • Cleaved into 2 chains
    • Assemblin
      (EC:3.4.21.97 (UniProtKB | ENZYME | Rhea)
      ) Alternative names: Protease
      (Pr
      )
    • Assembly protein
      (AP
      ) Alternative names: Capsid assembly protein

Gene names

    • Name
      ORF33
    • ORF names
      HHV3_E_SVETAgp35
      , HHV3gp35

Organism names

Accessions

  • Primary accession
    Q6QCM2

Proteomes

Subcellular Location

Capsid scaffolding protein

Host cytoplasm

Assembly protein

Host nucleus

Assemblin

Host nucleus

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_50405510181-236Assemblin
ChainPRO_50405510201-605Capsid scaffolding protein
ChainPRO_5040551019237-605Assembly protein

Post-translational modification

Capsid scaffolding protein: Capsid scaffolding protein is cleaved by assemblin after formation of the spherical procapsid. As a result, the capsid obtains its mature, icosahedral shape. Cleavages occur at two or more sites: release (R-site) and maturation (M-site).

Keywords

Interaction

Subunit

Assemblin

Exists in a monomer-dimer equilibrium with the dimer being the active species.

Assembly protein

Homomultimer. Interacts with major capsid protein.

Capsid scaffolding protein

Homomultimer. Interacts with major capsid protein.

Protein-protein interaction databases

Structure

3D structure databases

Family & Domains

Features

Showing features for region.

TypeIDPosition(s)Description
Region585-605Interaction with major capsid protein

Domain

Region of interaction between pPR and pAP is called Amino conserved domain (ACD). The region of interaction with major capsid protein is called carboxyl conserved domain (CCD).

Sequence similarities

Belongs to the herpesviridae capsid scaffolding protein family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    605
  • Mass (Da)
    66,047
  • Last updated
    2004-07-05 v1
  • MD5 Checksum
    819A9EF630FAD86B0E013AECEB8A1B79
MAAEADEENCEALYVAGYLALYSKDEGELNITPEIVRSALPPTSKIPINIDHRKDCVVGEVIAIIEDIRGPFFLGIVRCPQLHAVLFEAAHSNFFGNRDSVLSPLERALYLVTNYLPSVSLSSKRLSPNEIPDGNFFTHVALCVVGRRVGTVVNYDCTPESSIEPFRVLSMESKARLLSLVKDYAGLNKVWKVSEDKLAKVLLSTAVNNMLLRDRWDVVAKRRREAGIMGHVYLQASTGYGLARITNVNGVESKLPNAGVINATFHPGGPIYDLALGVGESNEDCEKTVPHLKVTQLCRNDSDMASVAGNASNISPQPPSGVPTGGEFVLIPTAYYSQLLTGQTKNPQVSIGAPNNGQYIVGPYGSPHPPAFPPNTGGYGCPPGHFGGPYGFPGYPPPNRLEMQMSAFMNALAAERGIDLQTPCVNFPDKTDVRRPGKRDFKSMDQRELDSFYSGESQMDGEFPSNIYFPGEPTYITHRRRRVSPSYWQRRHRVSNGQHEELAGVVAKLQQEVTELKSQNGTQMPLSHHTNIPEGTRDPRISILLKQLQSVSGLCSSQNTTSTPHTDTVGQDVNAVEASSKAPLIQGSTADDADMFANQMMVGRC

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AY548171
EMBL· GenBank· DDBJ
AAT07715.1
EMBL· GenBank· DDBJ
Genomic DNA
AY548170
EMBL· GenBank· DDBJ
AAT07791.1
EMBL· GenBank· DDBJ
Genomic DNA
DQ479953
EMBL· GenBank· DDBJ
ABF21533.1
EMBL· GenBank· DDBJ
Genomic DNA
DQ479954
EMBL· GenBank· DDBJ
ABF21606.1
EMBL· GenBank· DDBJ
Genomic DNA
DQ479955
EMBL· GenBank· DDBJ
ABF21679.1
EMBL· GenBank· DDBJ
Genomic DNA
DQ479956
EMBL· GenBank· DDBJ
ABF21752.1
EMBL· GenBank· DDBJ
Genomic DNA
DQ479958
EMBL· GenBank· DDBJ
ABF21898.1
EMBL· GenBank· DDBJ
Genomic DNA
DQ479959
EMBL· GenBank· DDBJ
ABF21971.1
EMBL· GenBank· DDBJ
Genomic DNA
DQ479961
EMBL· GenBank· DDBJ
ABF22117.1
EMBL· GenBank· DDBJ
Genomic DNA
DQ479962
EMBL· GenBank· DDBJ
ABF22190.1
EMBL· GenBank· DDBJ
Genomic DNA
DQ674250
EMBL· GenBank· DDBJ
ABH08485.1
EMBL· GenBank· DDBJ
Genomic DNA
EU154348
EMBL· GenBank· DDBJ
ABW06908.1
EMBL· GenBank· DDBJ
Genomic DNA
JN704690
EMBL· GenBank· DDBJ
AEW88010.1
EMBL· GenBank· DDBJ
Genomic DNA
JN704691
EMBL· GenBank· DDBJ
AEW88082.1
EMBL· GenBank· DDBJ
Genomic DNA
JN704692
EMBL· GenBank· DDBJ
AEW88154.1
EMBL· GenBank· DDBJ
Genomic DNA
JN704693
EMBL· GenBank· DDBJ
AEW88226.1
EMBL· GenBank· DDBJ
Genomic DNA
JN704694
EMBL· GenBank· DDBJ
AEW88298.1
EMBL· GenBank· DDBJ
Genomic DNA
JN704695
EMBL· GenBank· DDBJ
AEW88370.1
EMBL· GenBank· DDBJ
Genomic DNA
JN704699
EMBL· GenBank· DDBJ
AEW88658.1
EMBL· GenBank· DDBJ
Genomic DNA
JN704700
EMBL· GenBank· DDBJ
AEW88730.1
EMBL· GenBank· DDBJ
Genomic DNA
JN704701
EMBL· GenBank· DDBJ
AEW88802.1
EMBL· GenBank· DDBJ
Genomic DNA
JN704702
EMBL· GenBank· DDBJ
AEW88874.1
EMBL· GenBank· DDBJ
Genomic DNA
JN704703
EMBL· GenBank· DDBJ
AEW88946.1
EMBL· GenBank· DDBJ
Genomic DNA
JN704710
EMBL· GenBank· DDBJ
AEW89450.1
EMBL· GenBank· DDBJ
Genomic DNA
JQ972913
EMBL· GenBank· DDBJ
AFO85537.1
EMBL· GenBank· DDBJ
Genomic DNA
KP771889
EMBL· GenBank· DDBJ
AKG56102.1
EMBL· GenBank· DDBJ
Genomic DNA
KP771891
EMBL· GenBank· DDBJ
AKG56248.1
EMBL· GenBank· DDBJ
Genomic DNA
KP771892
EMBL· GenBank· DDBJ
AKG56321.1
EMBL· GenBank· DDBJ
Genomic DNA
KP771893
EMBL· GenBank· DDBJ
AKG56394.1
EMBL· GenBank· DDBJ
Genomic DNA
KP771894
EMBL· GenBank· DDBJ
AKG56467.1
EMBL· GenBank· DDBJ
Genomic DNA
KP771895
EMBL· GenBank· DDBJ
AKG56540.1
EMBL· GenBank· DDBJ
Genomic DNA
KP771896
EMBL· GenBank· DDBJ
AKG56613.1
EMBL· GenBank· DDBJ
Genomic DNA
KP771897
EMBL· GenBank· DDBJ
AKG56686.1
EMBL· GenBank· DDBJ
Genomic DNA
KP771899
EMBL· GenBank· DDBJ
AKG56832.1
EMBL· GenBank· DDBJ
Genomic DNA
KP771900
EMBL· GenBank· DDBJ
AKG56905.1
EMBL· GenBank· DDBJ
Genomic DNA
KP771901
EMBL· GenBank· DDBJ
AKG56978.1
EMBL· GenBank· DDBJ
Genomic DNA
KP771902
EMBL· GenBank· DDBJ
AKG57051.1
EMBL· GenBank· DDBJ
Genomic DNA
KP771903
EMBL· GenBank· DDBJ
AKG57124.1
EMBL· GenBank· DDBJ
Genomic DNA
KP771907
EMBL· GenBank· DDBJ
AKG57416.1
EMBL· GenBank· DDBJ
Genomic DNA
KP771908
EMBL· GenBank· DDBJ
AKG57489.1
EMBL· GenBank· DDBJ
Genomic DNA
KP771909
EMBL· GenBank· DDBJ
AKG57562.1
EMBL· GenBank· DDBJ
Genomic DNA
KP771910
EMBL· GenBank· DDBJ
AKG57635.1
EMBL· GenBank· DDBJ
Genomic DNA
KP771911
EMBL· GenBank· DDBJ
AKG57706.1
EMBL· GenBank· DDBJ
Genomic DNA
KP771912
EMBL· GenBank· DDBJ
AKG57779.1
EMBL· GenBank· DDBJ
Genomic DNA
KP771913
EMBL· GenBank· DDBJ
AKG57852.1
EMBL· GenBank· DDBJ
Genomic DNA
KP771915
EMBL· GenBank· DDBJ
AKG57996.1
EMBL· GenBank· DDBJ
Genomic DNA
KP771916
EMBL· GenBank· DDBJ
AKG58071.1
EMBL· GenBank· DDBJ
Genomic DNA
KP771917
EMBL· GenBank· DDBJ
AKG58144.1
EMBL· GenBank· DDBJ
Genomic DNA
KP771918
EMBL· GenBank· DDBJ
AKG58217.1
EMBL· GenBank· DDBJ
Genomic DNA
KP771920
EMBL· GenBank· DDBJ
AKG58363.1
EMBL· GenBank· DDBJ
Genomic DNA
KP771923
EMBL· GenBank· DDBJ
AKG58582.1
EMBL· GenBank· DDBJ
Genomic DNA
KU926311
EMBL· GenBank· DDBJ
ANS12905.1
EMBL· GenBank· DDBJ
Genomic DNA
KX262864
EMBL· GenBank· DDBJ
AON76499.1
EMBL· GenBank· DDBJ
Genomic DNA
KX262865
EMBL· GenBank· DDBJ
AON76572.1
EMBL· GenBank· DDBJ
Genomic DNA
KX262866
EMBL· GenBank· DDBJ
AON76645.1
EMBL· GenBank· DDBJ
Genomic DNA
MG764305
EMBL· GenBank· DDBJ
AYN61602.1
EMBL· GenBank· DDBJ
Genomic DNA
MG764312
EMBL· GenBank· DDBJ
AYN62113.1
EMBL· GenBank· DDBJ
Genomic DNA
MG764313
EMBL· GenBank· DDBJ
AYN62186.1
EMBL· GenBank· DDBJ
Genomic DNA
AJ871403
EMBL· GenBank· DDBJ
CAI44876.1
EMBL· GenBank· DDBJ
Genomic DNA
MH709312
EMBL· GenBank· DDBJ
QCA42951.1
EMBL· GenBank· DDBJ
Genomic DNA
MH709346
EMBL· GenBank· DDBJ
QCA45433.1
EMBL· GenBank· DDBJ
Genomic DNA
MH709354
EMBL· GenBank· DDBJ
QCA46017.1
EMBL· GenBank· DDBJ
Genomic DNA
MH709356
EMBL· GenBank· DDBJ
QCA46163.1
EMBL· GenBank· DDBJ
Genomic DNA
MH709361
EMBL· GenBank· DDBJ
QCA46528.1
EMBL· GenBank· DDBJ
Genomic DNA
MH709367
EMBL· GenBank· DDBJ
QCA46966.1
EMBL· GenBank· DDBJ
Genomic DNA
MH709369
EMBL· GenBank· DDBJ
QCA47112.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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