Q6QA32 · DHB3_DANRE
- Protein17-beta-hydroxysteroid dehydrogenase type 3
- Genehsd17b3
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids307 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Catalyzes the conversion of 17-oxosteroids to 17beta-hydroxysteroids in the presence of NADPH (PubMed:16216911, PubMed:27927697).
Favors the reduction of androstenedione to testosterone (PubMed:16216911, PubMed:27927697).
Testosterone is the key androgen driving male development and function (By similarity).
Among further tested androgens epiandrosterone and dehydroepiandrosterone are accepted as substrates and reduced at C-17 (PubMed:16216911).
Can also reduce 11-ketoandrostenedione as well as 11beta-hydroxyandrostenedione at C-17 to the respective testosterone forms (PubMed:16216911, PubMed:27927697).
Cannot use androsterone and androstanedione as substrates (PubMed:16216911).
Favors the reduction of androstenedione to testosterone (PubMed:16216911, PubMed:27927697).
Testosterone is the key androgen driving male development and function (By similarity).
Among further tested androgens epiandrosterone and dehydroepiandrosterone are accepted as substrates and reduced at C-17 (PubMed:16216911).
Can also reduce 11-ketoandrostenedione as well as 11beta-hydroxyandrostenedione at C-17 to the respective testosterone forms (PubMed:16216911, PubMed:27927697).
Cannot use androsterone and androstanedione as substrates (PubMed:16216911).
Catalytic activity
- a 17beta-hydroxy steroid + NADP+ = a 17-oxo steroid + H+ + NADPHThis reaction proceeds in the backward direction.
- 3beta-hydroxyandrost-5-en-17-one + H+ + NADPH = androst-5-en-3beta,17beta-diol + NADP+This reaction proceeds in the forward direction.
- 3beta-hydroxy-5alpha-androstan-17-one + H+ + NADPH = 5alpha-androstane-3beta,17beta-diol + NADP+This reaction proceeds in the forward direction.
- androst-4-ene-3,11,17-trione + H+ + NADPH = 17beta-hydroxyandrost-4-ene-3,11-dione + NADP+This reaction proceeds in the forward direction.
- 11beta-hydroxyandrost-4-ene-3,17-dione + H+ + NADPH = 11beta,17beta-dihydroxyandrost-4-ene-3-one + NADP+This reaction proceeds in the forward direction.
Pathway
Hormone biosynthesis; testosterone biosynthesis.
Steroid metabolism.
Features
Showing features for binding site, active site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | endoplasmic reticulum | |
Cellular Component | membrane | |
Molecular Function | testosterone 17-beta-dehydrogenase (NADP+) activity | |
Biological Process | androgen biosynthetic process | |
Biological Process | testosterone biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Chemistry
Names & Taxonomy
Protein names
- Recommended name17-beta-hydroxysteroid dehydrogenase type 3
- Short names17-beta-HSD 3
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Actinopterygii > Neopterygii > Teleostei > Ostariophysi > Cypriniformes > Danionidae > Danioninae > Danio
Accessions
- Primary accessionQ6QA32
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Membrane ; Single-pass membrane protein
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transmembrane | 6-26 | Helical | ||||
Sequence: IIFVLTGTCAILVFGGKIASL |
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000451342 | 1-307 | 17-beta-hydroxysteroid dehydrogenase type 3 | |||
Sequence: MTLTEIIFVLTGTCAILVFGGKIASLIMMLITKLFCPLPEAFFTSLGKWAVITGGSDGIGRAYAEELSKQGMSVIIISRNQEKLDRAAKKIELNTGGKVKVIAADFTKDDIYGHITENIEGLDIGVLVNNVGILPSQIPCKLLETSDLEERIYDIVNCNVKSMVKMCRIVLPGMQQRRRGVILNVSSGIAKIPCPIYTLYAASKVFVERFSQGLQAEYISKGIIIQTVAPFGVSTAMTGHQKPDMVTFTAEEFVRSSLKYLKTGDQTYGSITHTLLGRIVQSIPTWVLQSETFQHHFQEYVKNRDRR |
Expression
Tissue specificity
Expression shows strong sexual dimorphism. In female, highly expressed in ovaries, and at lower levels in skin muscle, eyes and liver (PubMed:16216911).
In males, strongly expressed in liver and at lower levels in testis, spleen, kidney, intestine and muscle (PubMed:16216911).
In males, strongly expressed in liver and at lower levels in testis, spleen, kidney, intestine and muscle (PubMed:16216911).
Developmental stage
Expressed in embryogenesis from sphere to 84 h post-fertilization.
Structure
Sequence
- Sequence statusComplete
- Length307
- Mass (Da)34,018
- Last updated2004-07-05 v1
- ChecksumD666B5904924C270
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
Q7T396 | Q7T396_DANRE | hsd17b3 | 236 |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CR376747 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
AY551081 EMBL· GenBank· DDBJ | AAS58451.1 EMBL· GenBank· DDBJ | mRNA |