Q6Q2Z6 · ACOT5_MOUSE
- ProteinAcyl-coenzyme A thioesterase 5
- GeneAcot5
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids421 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Catalyzes the hydrolysis of acyl-CoAs into free fatty acids and coenzyme A (CoASH), regulating their respective intracellular levels (PubMed:16940157).
Mainly active on medium-chain acyl-CoAs (PubMed:15007068).
Seems to be involved in intraperoxisomal regulation of acyl-CoA levels, but not CoASH levels (PubMed:15007068).
May have a function in termination of beta-oxidation of fatty acids (PubMed:15007068).
Mainly active on medium-chain acyl-CoAs (PubMed:15007068).
Seems to be involved in intraperoxisomal regulation of acyl-CoA levels, but not CoASH levels (PubMed:15007068).
May have a function in termination of beta-oxidation of fatty acids (PubMed:15007068).
Catalytic activity
- H2O + hexadecanoyl-CoA = CoA + H+ + hexadecanoateThis reaction proceeds in the forward direction.
- decanoyl-CoA + H2O = CoA + decanoate + H+This reaction proceeds in the forward direction.
- H2O + octanoyl-CoA = CoA + H+ + octanoateThis reaction proceeds in the forward direction.
- dodecanoyl-CoA + H2O = CoA + dodecanoate + H+This reaction proceeds in the forward direction.
- H2O + tetradecanoyl-CoA = CoA + H+ + tetradecanoateThis reaction proceeds in the forward direction.
- H2O + octadecanoyl-CoA = CoA + H+ + octadecanoateThis reaction proceeds in the forward direction.
- eicosanoyl-CoA + H2O = CoA + eicosanoate + H+This reaction proceeds in the forward direction.
- (9Z)-octadecenoyl-CoA + H2O = (9Z)-octadecenoate + CoA + H+This reaction proceeds in the forward direction.
- (9Z,12Z)-octadecadienoyl-CoA + H2O = (9Z,12Z)-octadecadienoate + CoA + H+This reaction proceeds in the forward direction.
- (5Z,8Z,11Z,14Z)-eicosatetraenoyl-CoA + H2O = (5Z,8Z,11Z,14Z)-eicosatetraenoate + CoA + H+This reaction proceeds in the forward direction.
- (9Z)-hexadecenoyl-CoA + H2O = (9Z)-hexadecenoate + CoA + H+This reaction proceeds in the forward direction.
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
28.4 μM | hexanoyl-coA | |||||
7.7 μM | octanoyl-coA | |||||
18 μM | decanoyl-coA | |||||
7.6 μM | dodecanoyl-coA | |||||
5.6 μM | tetradecanoyl-coA | |||||
15.9 μM | hexadecanoyl-coA | |||||
7 μM | (9Z)-hexadecenoyl-coA | |||||
34.8 μM | octadecanoyl-coA | |||||
8.9 μM | (9Z)-octadecenoyl-coA | |||||
32.5 μM | (9Z,12Z)-octadecadienoyl-coA | |||||
32.7 μM | eicosanoyl-coA | |||||
1.6 μM | (5Z,8Z,11Z,14Z)-eicosatetraenoyl-coA |
Vmax | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|
0.23 μmol/min/mg | with hexanoyl-coA as substrate | ||||
0.74 μmol/min/mg | with octanoyl-coA as substrate | ||||
1.2 μmol/min/mg | with decanoyl-coA as substrate | ||||
1.1 μmol/min/mg | with dodecanoyl-coA as substrate | ||||
0.83 μmol/min/mg | with tetradecanoyl-coA as substrate | ||||
0.49 μmol/min/mg | with hexadecanoyl-coA as substrate | ||||
0.32 μmol/min/mg | with (9Z)-hexadecenoyl-coA as substrate | ||||
0.38 μmol/min/mg | with octadecanoyl-coA as substrate | ||||
0.14 μmol/min/mg | with (9Z)-octadecenoyl-coA as substrate | ||||
0.17 μmol/min/mg | with (9Z,12Z)-octadecadienoyl-coA as substrate | ||||
0.18 μmol/min/mg | with eicosanoyl-coA as substrate | ||||
0.23 μmol/min/mg | with (5Z,8Z,11Z,14Z)-eicosatetraenoyl-coA as substrate |
Pathway
Lipid metabolism; fatty acid metabolism.
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 232 | Charge relay system | ||||
Sequence: S | ||||||
Active site | 326 | Charge relay system | ||||
Sequence: D | ||||||
Active site | 360 | Charge relay system | ||||
Sequence: H |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Cellular Component | peroxisome | |
Molecular Function | carboxylic ester hydrolase activity | |
Molecular Function | fatty acyl-CoA hydrolase activity | |
Biological Process | acyl-CoA metabolic process | |
Biological Process | fatty acid metabolic process | |
Biological Process | long-chain fatty acid metabolic process | |
Biological Process | saturated monocarboxylic acid metabolic process | |
Biological Process | unsaturated monocarboxylic acid metabolic process | |
Biological Process | very long-chain fatty acid metabolic process |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Protein family/group databases
Chemistry
Names & Taxonomy
Protein names
- Recommended nameAcyl-coenzyme A thioesterase 5
- EC number
- Short namesAcyl-CoA thioesterase 5
- Alternative names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionQ6Q2Z6
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000202151 | 1-421 | Acyl-coenzyme A thioesterase 5 | |||
Sequence: MVPTVSLEPTGHSCWDEPLSIAVRGLAPEQPVTLRTALRDEKGALFRAHARYRADSHGELDLARTPALGGSFSGLEPMGLLWAMEPDRPFWRLIKRDVQTPFVVELEVLDGHEPDGGRLLARAVHERHFMAPGVRRVPVREGRVRATLFLPPGTGPFPGIIDLFGVGGGLLEYRASLLAGKGFAVMALAYYKYDDLPKVIDILHLEYFEEAVTYLLSHPQVKGPGVGLLGISKGAELSLSMASFLKGITAAVVINGATVNVISTLYYKEESLPGLGMHLERIKVTKDGFKDIIDILNVPLEAPDQKSLIPLERSDTAFLFLVGQDDHNWKSEFYAREASKRLQAHGKEKPQIVCYPKTGHHIEPPYIPWSIAAPHSYFDKPILLGGEPRAHAMAQVDAWQRLQTFFHKHLSGDKRPSPAKL |
Proteomic databases
PTM databases
Expression
Tissue specificity
Highly expressed in spleen, brain, testis and proximal and distal intestine; expressed at low level in the liver.
Induction
In the liver and kidney, by peroxisome proliferator, via the peroxisome proliferator-activated receptors (PPARs) and by fasting.
Gene expression databases
Structure
Family & Domains
Features
Showing features for motif.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Motif | 419-421 | Microbody targeting signal | ||||
Sequence: AKL |
Sequence similarities
Belongs to the C/M/P thioester hydrolase family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length421
- Mass (Da)46,573
- Last updated2011-07-27 v2
- Checksum3156BA777FFBEE94
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
Q91YQ6 | Q91YQ6_MOUSE | Acot5 | 331 |
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 127 | in Ref. 1; AAS75457 | ||||
Sequence: R → C |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AY563099 EMBL· GenBank· DDBJ | AAS75457.1 EMBL· GenBank· DDBJ | mRNA | ||
AC125071 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. |