Q6PGN3 · DCLK2_MOUSE
- ProteinSerine/threonine-protein kinase DCLK2
- GeneDclk2
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids756 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Protein kinase with a significantly reduced Ca2++/CAM affinity and dependence compared to other members of the CaMK family. May play a role in the down-regulation of CRE-dependent gene activation probably by phosphorylation of the CREB coactivator CRTC2/TORC2 and the resulting retention of TORC2 in the cytoplasm.
Catalytic activity
- ATP + L-seryl-[protein] = ADP + H+ + O-phospho-L-seryl-[protein]
Features
Showing features for binding site, active site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | cytoskeleton | |
Molecular Function | ATP binding | |
Molecular Function | microtubule binding | |
Molecular Function | protein kinase activity | |
Molecular Function | protein serine kinase activity | |
Molecular Function | protein serine/threonine kinase activity | |
Biological Process | hippocampus development | |
Biological Process | intracellular signal transduction | |
Biological Process | microtubule cytoskeleton organization | |
Biological Process | negative regulation of protein localization to nucleus | |
Biological Process | protein localization to nucleus | |
Biological Process | pyramidal neuron development |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameSerine/threonine-protein kinase DCLK2
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionQ6PGN3
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Note: Colocalizes with microtubules.
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
Frequent spontaneous seizures that originate in the hippocampus, with most animals dying in the first few months of life.
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 422 | Loss of kinase activity. No effect on colocalization with microtubules. | ||||
Sequence: K → A |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 40 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000085923 | 1-756 | Serine/threonine-protein kinase DCLK2 | |||
Sequence: MASTRSIELEHFEERDKRPRPGSRRGAPSSSGGSSISGPKGNGLIPSPAHSAHCSFYRTRTLQALSSEKKAKKARFYRNGDRYFKGLVFAISNDRFRSFDALLIELTRSLSDNVNLPQGVRTIYTIDGSRKVTSLDELLEGESYVCASNEPFRKVDYTKNVNPNWSVNIKGGTTRTLAVASAKSEVKESKDFIKPKLVTVIRSGVKPRKAVRILLNKKTAHSFEQVLTDITEAIKLDSGVVKRLCTLDGKQVTCLQDFFGDDDVFIACGPEKYRYAQDDFVLDHSECRVLKSSYSRASAAKYSGSRSPGFSRRSKSPASVNGTPSSQLSTPKSTKSSSSSPTSPGSFRGLKQISAQGRSSSNVNGGPELDRCLSPEGVNGNRCSESFPLLEKYRIGKVIGDGNFAVVKECVDRYTGKEFALKIIDKAKCCGKEHLIENEVSILRRVKHPNIIMLVEEMETATDLFLVMELVKGGDLFDAITSSTKYTERDGSAMVYNLANALRYLHSLSIVHRDIKPENLLVCEYPDGTKSLKLGDFGLATVVEGPLYTVCGTPTYVAPEIIAETGYGLKVDVWAAGVITYILLCGFPPFRSENNLQEDLFDQILAGKLEFPAPYWDNITDSAKELISQMLQVNVEARCTAGEILSHPWVSDDASQENNMQAEVTGKLKQHFNNALPKQNSTTTGVSVIMNTALDKEGQIFCSKLCQDSSRPSREQTSPVPPSAQEAPPPLESPRPPGPPATSGCDLAGTWRRHRD | ||||||
Modified residue | 61 | Phosphothreonine | ||||
Sequence: T | ||||||
Modified residue | 361 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 646 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 665 | Phosphothreonine | ||||
Sequence: T |
Post-translational modification
Autophosphorylated.
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
Expressed in the central and peripheral nervous system including the brain, spinal cord, cranial and dorsal root ganglia and in the parasympathetic ganglia. Present in neurons, but not in glial cells, in most forebrain areas. Strong expression in the hippocampal CA1 pyramidal cell layer. Expressed in the photoreceptor sensory cilium complex and in eyes. Also detected in individual cells of the olfactory epithelium.
Developmental stage
At 17.5 dpc, predominantly expressed in the central nervous system, throughout the forebrain, midbrain, hindbrain, and the spinal cord. Expressed in the developing neocortex and at low levels in the ventricular zone, especially in the outer neuroblastic layer. In the developing retina, strongly expressed in the postmitotic inner neuroblastic layer. Also found in the developing ovary and, to a lower extent, throughout the kidney.
Gene expression databases
Structure
Family & Domains
Features
Showing features for region, compositional bias, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-44 | Disordered | ||||
Sequence: MASTRSIELEHFEERDKRPRPGSRRGAPSSSGGSSISGPKGNGL | ||||||
Compositional bias | 7-23 | Basic and acidic residues | ||||
Sequence: IELEHFEERDKRPRPGS | ||||||
Compositional bias | 27-41 | Polar residues | ||||
Sequence: APSSSGGSSISGPKG | ||||||
Domain | 72-158 | Doublecortin 1 | ||||
Sequence: KKARFYRNGDRYFKGLVFAISNDRFRSFDALLIELTRSLSDNVNLPQGVRTIYTIDGSRKVTSLDELLEGESYVCASNEPFRKVDYT | ||||||
Domain | 196-279 | Doublecortin 2 | ||||
Sequence: KLVTVIRSGVKPRKAVRILLNKKTAHSFEQVLTDITEAIKLDSGVVKRLCTLDGKQVTCLQDFFGDDDVFIACGPEKYRYAQDD | ||||||
Compositional bias | 301-367 | Polar residues | ||||
Sequence: KYSGSRSPGFSRRSKSPASVNGTPSSQLSTPKSTKSSSSSPTSPGSFRGLKQISAQGRSSSNVNGGP | ||||||
Region | 301-375 | Disordered | ||||
Sequence: KYSGSRSPGFSRRSKSPASVNGTPSSQLSTPKSTKSSSSSPTSPGSFRGLKQISAQGRSSSNVNGGPELDRCLSP | ||||||
Domain | 393-650 | Protein kinase | ||||
Sequence: YRIGKVIGDGNFAVVKECVDRYTGKEFALKIIDKAKCCGKEHLIENEVSILRRVKHPNIIMLVEEMETATDLFLVMELVKGGDLFDAITSSTKYTERDGSAMVYNLANALRYLHSLSIVHRDIKPENLLVCEYPDGTKSLKLGDFGLATVVEGPLYTVCGTPTYVAPEIIAETGYGLKVDVWAAGVITYILLCGFPPFRSENNLQEDLFDQILAGKLEFPAPYWDNITDSAKELISQMLQVNVEARCTAGEILSHPWV | ||||||
Compositional bias | 707-721 | Polar residues | ||||
Sequence: QDSSRPSREQTSPVP | ||||||
Region | 707-756 | Disordered | ||||
Sequence: QDSSRPSREQTSPVPPSAQEAPPPLESPRPPGPPATSGCDLAGTWRRHRD | ||||||
Compositional bias | 722-741 | Pro residues | ||||
Sequence: PSAQEAPPPLESPRPPGPPA |
Domain
The doublecortin domains are involved in the colocalization with microtubules.
Sequence similarities
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence & Isoforms
- Sequence statusComplete
This entry describes 6 isoforms produced by Alternative splicing.
Q6PGN3-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- Length756
- Mass (Da)82,979
- Last updated2004-07-05 v1
- ChecksumC658F0999547B779
Q6PGN3-2
- Name2
- Differences from canonical
- 352-352: Missing
Q6PGN3-3
- Name3
- Synonymsalpha
Q6PGN3-4
- Name4
- Synonymsbeta1
Q6PGN3-5
- Name5
- Synonymsbeta2
Q6PGN3-6
- Name6
Computationally mapped potential isoform sequences
There are 3 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A0A6YWI6 | A0A0A6YWI6_MOUSE | Dclk2 | 641 | ||
A0A0A6YX71 | A0A0A6YX71_MOUSE | Dclk2 | 711 | ||
A0A0A6YX33 | A0A0A6YX33_MOUSE | Dclk2 | 591 |
Features
Showing features for compositional bias, sequence conflict, alternative sequence.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 7-23 | Basic and acidic residues | ||||
Sequence: IELEHFEERDKRPRPGS | ||||||
Compositional bias | 27-41 | Polar residues | ||||
Sequence: APSSSGGSSISGPKG | ||||||
Sequence conflict | 33 | in Ref. 1; AAY40245 | ||||
Sequence: G → D | ||||||
Compositional bias | 301-367 | Polar residues | ||||
Sequence: KYSGSRSPGFSRRSKSPASVNGTPSSQLSTPKSTKSSSSSPTSPGSFRGLKQISAQGRSSSNVNGGP | ||||||
Alternative sequence | VSP_038892 | 320 | in isoform 3, isoform 4 and isoform 5 | |||
Sequence: V → VKRAGHSSAYSTAKSPV | ||||||
Alternative sequence | VSP_012797 | 352 | in isoform 2, isoform 3 and isoform 5 | |||
Sequence: Missing | ||||||
Alternative sequence | VSP_038893 | 625-651 | in isoform 6 | |||
Sequence: Missing | ||||||
Alternative sequence | VSP_038895 | 691-699 | in isoform 4 and isoform 5 | |||
Sequence: NTALDKEGQ → VQGHEHGSR | ||||||
Alternative sequence | VSP_038894 | 691-756 | in isoform 6 | |||
Sequence: NTALDKEGQIFCSKLCQDSSRPSREQTSPVPPSAQEAPPPLESPRPPGPPATSGCDLAGTWRRHRD → VSGTQSSASESRGWPSWSCCLDSQGSAHGSWCLPCSCLHGGLPGM | ||||||
Alternative sequence | VSP_038896 | 700-756 | in isoform 4 and isoform 5 | |||
Sequence: Missing | ||||||
Compositional bias | 707-721 | Polar residues | ||||
Sequence: QDSSRPSREQTSPVP | ||||||
Compositional bias | 722-741 | Pro residues | ||||
Sequence: PSAQEAPPPLESPRPPGPPA |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AY968049 EMBL· GenBank· DDBJ | AAY40243.1 EMBL· GenBank· DDBJ | mRNA | ||
AY968050 EMBL· GenBank· DDBJ | AAY40244.1 EMBL· GenBank· DDBJ | mRNA | ||
AY968051 EMBL· GenBank· DDBJ | AAY40245.1 EMBL· GenBank· DDBJ | mRNA | ||
AB198721 EMBL· GenBank· DDBJ | BAE06836.1 EMBL· GenBank· DDBJ | mRNA | ||
AK049179 EMBL· GenBank· DDBJ | BAC33590.1 EMBL· GenBank· DDBJ | mRNA | ||
AK082633 EMBL· GenBank· DDBJ | BAC38555.1 EMBL· GenBank· DDBJ | mRNA | ||
BC056921 EMBL· GenBank· DDBJ | AAH56921.1 EMBL· GenBank· DDBJ | mRNA |