Q6PGN3 · DCLK2_MOUSE

  • Protein
    Serine/threonine-protein kinase DCLK2
  • Gene
    Dclk2
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Protein kinase with a significantly reduced Ca2++/CAM affinity and dependence compared to other members of the CaMK family. May play a role in the down-regulation of CRE-dependent gene activation probably by phosphorylation of the CREB coactivator CRTC2/TORC2 and the resulting retention of TORC2 in the cytoplasm.

Catalytic activity

Features

Showing features for binding site, active site.

1756100200300400500600700
TypeIDPosition(s)Description
Binding site399-407ATP (UniProtKB | ChEBI)
Binding site422ATP (UniProtKB | ChEBI)
Active site514Proton acceptor

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular Componentcytoskeleton
Molecular FunctionATP binding
Molecular Functionmicrotubule binding
Molecular Functionprotein kinase activity
Molecular Functionprotein serine kinase activity
Molecular Functionprotein serine/threonine kinase activity
Biological Processhippocampus development
Biological Processintracellular signal transduction
Biological Processmicrotubule cytoskeleton organization
Biological Processnegative regulation of protein localization to nucleus
Biological Processprotein localization to nucleus
Biological Processpyramidal neuron development

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Serine/threonine-protein kinase DCLK2
  • EC number
  • Alternative names
    • CaMK-like CREB regulatory kinase 2 (CL2; CLICK-II; CLICK2)
    • Doublecortin-like and CAM kinase-like 2
    • Doublecortin-like kinase 2

Gene names

    • Name
      Dclk2
    • Synonyms
      Dcamkl2

Organism names

  • Taxonomic identifier
  • Strains
    • ICR
    • C57BL/6J
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus

Accessions

  • Primary accession
    Q6PGN3
  • Secondary accessions
    • Q1EDG7
    • Q1EDG8
    • Q4H483
    • Q4W8V1
    • Q8BUU0
    • Q8BX25

Proteomes

Organism-specific databases

Subcellular Location

Keywords

Phenotypes & Variants

Disruption phenotype

Frequent spontaneous seizures that originate in the hippocampus, with most animals dying in the first few months of life.

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis422Loss of kinase activity. No effect on colocalization with microtubules.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 40 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain, modified residue.

TypeIDPosition(s)Description
ChainPRO_00000859231-756Serine/threonine-protein kinase DCLK2
Modified residue61Phosphothreonine
Modified residue361Phosphoserine
Modified residue646Phosphoserine
Modified residue665Phosphothreonine

Post-translational modification

Autophosphorylated.

Keywords

Proteomic databases

PTM databases

Expression

Tissue specificity

Expressed in the central and peripheral nervous system including the brain, spinal cord, cranial and dorsal root ganglia and in the parasympathetic ganglia. Present in neurons, but not in glial cells, in most forebrain areas. Strong expression in the hippocampal CA1 pyramidal cell layer. Expressed in the photoreceptor sensory cilium complex and in eyes. Also detected in individual cells of the olfactory epithelium.

Developmental stage

At 17.5 dpc, predominantly expressed in the central nervous system, throughout the forebrain, midbrain, hindbrain, and the spinal cord. Expressed in the developing neocortex and at low levels in the ventricular zone, especially in the outer neuroblastic layer. In the developing retina, strongly expressed in the postmitotic inner neuroblastic layer. Also found in the developing ovary and, to a lower extent, throughout the kidney.

Gene expression databases

Interaction

Subunit

Binds to and stabilizes microtubules (By similarity).
Interacts with MAPK8IP1/JIP-1, MAPK8IP2/JIP-2, MAPK9/JNK2, PPP1R9B/NEURABIN-2 and actin

Protein-protein interaction databases

Miscellaneous

Structure

Family & Domains

Features

Showing features for region, compositional bias, domain.

TypeIDPosition(s)Description
Region1-44Disordered
Compositional bias7-23Basic and acidic residues
Compositional bias27-41Polar residues
Domain72-158Doublecortin 1
Domain196-279Doublecortin 2
Compositional bias301-367Polar residues
Region301-375Disordered
Domain393-650Protein kinase
Compositional bias707-721Polar residues
Region707-756Disordered
Compositional bias722-741Pro residues

Domain

The doublecortin domains are involved in the colocalization with microtubules.

Sequence similarities

Keywords

Phylogenomic databases

Family and domain databases

Sequence & Isoforms

Align isoforms (6)
  • Sequence status
    Complete

This entry describes 6 isoforms produced by Alternative splicing.

Q6PGN3-1

This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

  • Length
    756
  • Mass (Da)
    82,979
  • Last updated
    2004-07-05 v1
  • Checksum
    C658F0999547B779
MASTRSIELEHFEERDKRPRPGSRRGAPSSSGGSSISGPKGNGLIPSPAHSAHCSFYRTRTLQALSSEKKAKKARFYRNGDRYFKGLVFAISNDRFRSFDALLIELTRSLSDNVNLPQGVRTIYTIDGSRKVTSLDELLEGESYVCASNEPFRKVDYTKNVNPNWSVNIKGGTTRTLAVASAKSEVKESKDFIKPKLVTVIRSGVKPRKAVRILLNKKTAHSFEQVLTDITEAIKLDSGVVKRLCTLDGKQVTCLQDFFGDDDVFIACGPEKYRYAQDDFVLDHSECRVLKSSYSRASAAKYSGSRSPGFSRRSKSPASVNGTPSSQLSTPKSTKSSSSSPTSPGSFRGLKQISAQGRSSSNVNGGPELDRCLSPEGVNGNRCSESFPLLEKYRIGKVIGDGNFAVVKECVDRYTGKEFALKIIDKAKCCGKEHLIENEVSILRRVKHPNIIMLVEEMETATDLFLVMELVKGGDLFDAITSSTKYTERDGSAMVYNLANALRYLHSLSIVHRDIKPENLLVCEYPDGTKSLKLGDFGLATVVEGPLYTVCGTPTYVAPEIIAETGYGLKVDVWAAGVITYILLCGFPPFRSENNLQEDLFDQILAGKLEFPAPYWDNITDSAKELISQMLQVNVEARCTAGEILSHPWVSDDASQENNMQAEVTGKLKQHFNNALPKQNSTTTGVSVIMNTALDKEGQIFCSKLCQDSSRPSREQTSPVPPSAQEAPPPLESPRPPGPPATSGCDLAGTWRRHRD

Q6PGN3-2

  • Name
    2
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical

Q6PGN3-3

  • Name
    3
  • Synonyms
    alpha
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical

Q6PGN3-4

  • Name
    4
  • Synonyms
    beta1
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical

Q6PGN3-5

  • Name
    5
  • Synonyms
    beta2
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical

Q6PGN3-6

  • Name
    6
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical
    • 625-651: Missing
    • 691-756: NTALDKEGQIFCSKLCQDSSRPSREQTSPVPPSAQEAPPPLESPRPPGPPATSGCDLAGTWRRHRD → VSGTQSSASESRGWPSWSCCLDSQGSAHGSWCLPCSCLHGGLPGM

Computationally mapped potential isoform sequences

There are 3 potential isoforms mapped to this entry

View all
EntryEntry nameGene nameLength
A0A0A6YWI6A0A0A6YWI6_MOUSEDclk2641
A0A0A6YX71A0A0A6YX71_MOUSEDclk2711
A0A0A6YX33A0A0A6YX33_MOUSEDclk2591

Features

Showing features for compositional bias, sequence conflict, alternative sequence.

TypeIDPosition(s)Description
Compositional bias7-23Basic and acidic residues
Compositional bias27-41Polar residues
Sequence conflict33in Ref. 1; AAY40245
Compositional bias301-367Polar residues
Alternative sequenceVSP_038892320in isoform 3, isoform 4 and isoform 5
Alternative sequenceVSP_012797352in isoform 2, isoform 3 and isoform 5
Alternative sequenceVSP_038893625-651in isoform 6
Alternative sequenceVSP_038895691-699in isoform 4 and isoform 5
Alternative sequenceVSP_038894691-756in isoform 6
Alternative sequenceVSP_038896700-756in isoform 4 and isoform 5
Compositional bias707-721Polar residues
Compositional bias722-741Pro residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AY968049
EMBL· GenBank· DDBJ
AAY40243.1
EMBL· GenBank· DDBJ
mRNA
AY968050
EMBL· GenBank· DDBJ
AAY40244.1
EMBL· GenBank· DDBJ
mRNA
AY968051
EMBL· GenBank· DDBJ
AAY40245.1
EMBL· GenBank· DDBJ
mRNA
AB198721
EMBL· GenBank· DDBJ
BAE06836.1
EMBL· GenBank· DDBJ
mRNA
AK049179
EMBL· GenBank· DDBJ
BAC33590.1
EMBL· GenBank· DDBJ
mRNA
AK082633
EMBL· GenBank· DDBJ
BAC38555.1
EMBL· GenBank· DDBJ
mRNA
BC056921
EMBL· GenBank· DDBJ
AAH56921.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

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