Q6PER3 · MARE3_MOUSE
- ProteinMicrotubule-associated protein RP/EB family member 3
- GeneMapre3
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids281 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Plus-end tracking protein (+TIP) that binds to the plus-end of microtubules and regulates the dynamics of the microtubule cytoskeleton. Promotes microtubule growth. May be involved in spindle function by stabilizing microtubules and anchoring them at centrosomes. Also acts as a regulator of minus-end microtubule organization: interacts with the complex formed by AKAP9 and PDE4DIP, leading to recruit CAMSAP2 to the Golgi apparatus, thereby tethering non-centrosomal minus-end microtubules to the Golgi, an important step for polarized cell movement. Promotes elongation of CAMSAP2-decorated microtubule stretches on the minus-end of microtubules (By similarity).
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | cytoplasmic microtubule | |
Cellular Component | microtubule cytoskeleton | |
Cellular Component | microtubule organizing center | |
Cellular Component | microtubule plus-end | |
Cellular Component | midbody | |
Cellular Component | mitotic spindle astral microtubule end | |
Cellular Component | spindle midzone | |
Molecular Function | identical protein binding | |
Molecular Function | microtubule binding | |
Molecular Function | microtubule plus-end binding | |
Molecular Function | protein kinase binding | |
Biological Process | cell division | |
Biological Process | positive regulation of cyclin-dependent protein serine/threonine kinase activity | |
Biological Process | positive regulation of DNA-templated transcription | |
Biological Process | protein localization to microtubule | |
Biological Process | regulation of microtubule polymerization | |
Biological Process | regulation of microtubule polymerization or depolymerization | |
Biological Process | spindle assembly |
Keywords
- Biological process
Names & Taxonomy
Protein names
- Recommended nameMicrotubule-associated protein RP/EB family member 3
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionQ6PER3
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Note: Associated with the microtubule network. Detected at the plus end of microtubules.
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000213429 | 1-281 | Microtubule-associated protein RP/EB family member 3 | |||
Sequence: MAVNVYSTSVTSENLSRHDMLAWVNDSLHLNYTKIEQLCSGAAYCQFMDMLFPGCVHLRKVKFQAKLEHEYIHNFKVLQAAFKKMGVDKIIPVEKLVKGKFQDNFEFIQWFKKFFDANYDGKDYNPLLARQGQDVAPPPNPGDQIFNKSKKLIGTAVPQRTSPTGPKNMQTSGRLSNVAPPCILRKNPPSARNGGHEADAQILELNQQLLDLKLTVDGLEKERDFYFSKLRDIELICQEHESENSPVISGIIGILYATEEGFAPPEDDEIEEHQQEDQDEY | ||||||
Modified residue | 162 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 176 | Phosphoserine | ||||
Sequence: S |
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Gene expression databases
Interaction
Subunit
Homodimer (By similarity).
Heterodimer with MAPRE1 (By similarity).
Binds monomeric and polymerized GTP-bound tubulin. Interacts with DCTN1 and SRCIN1 (By similarity).
Binds to the C-terminal domain of APC (By similarity).
Interacts (via C-terminus) with CLIP1 (By similarity).
Interacts with SLAIN2 (By similarity).
Interacts with SLAIN1 (PubMed:21646404).
Interacts with APC2 (PubMed:17310996).
Interacts with AKAP9 (By similarity).
Interacts with PDE4DIP isoform 2/MMG8/SMYLE; this interaction is required for its recruitment to the Golgi apparatus (By similarity).
Heterodimer with MAPRE1 (By similarity).
Binds monomeric and polymerized GTP-bound tubulin. Interacts with DCTN1 and SRCIN1 (By similarity).
Binds to the C-terminal domain of APC (By similarity).
Interacts (via C-terminus) with CLIP1 (By similarity).
Interacts with SLAIN2 (By similarity).
Interacts with SLAIN1 (PubMed:21646404).
Interacts with APC2 (PubMed:17310996).
Interacts with AKAP9 (By similarity).
Interacts with PDE4DIP isoform 2/MMG8/SMYLE; this interaction is required for its recruitment to the Golgi apparatus (By similarity).
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for domain, compositional bias, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 14-116 | Calponin-homology (CH) | ||||
Sequence: NLSRHDMLAWVNDSLHLNYTKIEQLCSGAAYCQFMDMLFPGCVHLRKVKFQAKLEHEYIHNFKVLQAAFKKMGVDKIIPVEKLVKGKFQDNFEFIQWFKKFFD | ||||||
Compositional bias | 157-177 | Polar residues | ||||
Sequence: VPQRTSPTGPKNMQTSGRLSN | ||||||
Region | 157-181 | Disordered | ||||
Sequence: VPQRTSPTGPKNMQTSGRLSNVAPP | ||||||
Domain | 194-264 | EB1 C-terminal | ||||
Sequence: GGHEADAQILELNQQLLDLKLTVDGLEKERDFYFSKLRDIELICQEHESENSPVISGIIGILYATEEGFAP | ||||||
Region | 217-260 | APC-binding | ||||
Sequence: DGLEKERDFYFSKLRDIELICQEHESENSPVISGIIGILYATEE | ||||||
Region | 217-281 | DCTN1-binding | ||||
Sequence: DGLEKERDFYFSKLRDIELICQEHESENSPVISGIIGILYATEEGFAPPEDDEIEEHQQEDQDEY | ||||||
Region | 260-281 | Disordered | ||||
Sequence: EGFAPPEDDEIEEHQQEDQDEY | ||||||
Compositional bias | 262-281 | Acidic residues | ||||
Sequence: FAPPEDDEIEEHQQEDQDEY |
Domain
Composed of two functionally independent domains. The N-terminal domain forms a hydrophobic cleft involved in microtubule binding and the C-terminal is involved in the formation of mutually exclusive complexes with APC and DCTN1.
Sequence similarities
Belongs to the MAPRE family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length281
- Mass (Da)31,966
- Last updated2004-07-05 v1
- Checksum6713427C480838DC
Computationally mapped potential isoform sequences
There are 2 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A0J9YVB9 | A0A0J9YVB9_MOUSE | Mapre3 | 140 | ||
D3Z6G3 | D3Z6G3_MOUSE | Mapre3 | 266 |
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 157-177 | Polar residues | ||||
Sequence: VPQRTSPTGPKNMQTSGRLSN | ||||||
Compositional bias | 262-281 | Acidic residues | ||||
Sequence: FAPPEDDEIEEHQQEDQDEY |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
BC057918 EMBL· GenBank· DDBJ | AAH57918.1 EMBL· GenBank· DDBJ | mRNA | ||
U51204 EMBL· GenBank· DDBJ | AAA96321.1 EMBL· GenBank· DDBJ | mRNA |