Q6PEE6 · Q6PEE6_MOUSE
- ProteinAP-2 complex subunit alpha
- GeneAp2a2
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids938 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score2/5
Function
function
Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha subunit binds polyphosphoinositide-containing lipids, positioning AP-2 on the membrane. The AP-2 alpha subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 11-12 | a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3,4,5-trisphosphate) (UniProtKB | ChEBI) | |||
Binding site | 43 | a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3,4,5-trisphosphate) (UniProtKB | ChEBI) | |||
Binding site | 53 | a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3,4,5-trisphosphate) (UniProtKB | ChEBI) | |||
Binding site | 57-61 | a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3,4,5-trisphosphate) (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | AP-2 adaptor complex | |
Molecular Function | clathrin adaptor activity | |
Biological Process | clathrin-dependent endocytosis | |
Biological Process | intracellular protein transport |
Keywords
- Biological process
Names & Taxonomy
Protein names
- Recommended nameAP-2 complex subunit alpha
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionQ6PEE6
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Membrane, coated pit ; Peripheral membrane protein
Keywords
- Cellular component
PTM/Processing
Proteomic databases
Interaction
Subunit
Adaptor protein complex 2 (AP-2) is a heterotetramer composed of two large adaptins (alpha-type subunit AP2A1 or AP2A2 and beta-type subunit AP2B1), a medium adaptin (mu-type subunit AP2M1) and a small adaptin (sigma-type subunit AP2S1).
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for compositional bias, region, domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Compositional bias | 615-638 | Basic and acidic residues | |||
Region | 615-681 | Disordered | |||
Domain | 706-819 | Clathrin adaptor alpha/beta/gamma-adaptin appendage Ig-like subdomain | |||
Sequence similarities
Belongs to the adaptor complexes large subunit family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length938
- Mass (Da)104,002
- Last updated2004-07-05 v1
- MD5 ChecksumD718896D0A506125E81CDA415FEA3564
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Compositional bias | 615-638 | Basic and acidic residues | |||
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
BC058099 EMBL· GenBank· DDBJ | AAH58099.1 EMBL· GenBank· DDBJ | mRNA |