Q6PCN7 · HLTF_MOUSE

  • Protein
    Helicase-like transcription factor
  • Gene
    Hltf
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Has both helicase and E3 ubiquitin ligase activities. Possesses intrinsic ATP-dependent nucleosome-remodeling activity. This activity may be required for transcriptional activation or repression of specific target promoters (By similarity).
These may include the SERPINE1, to which this protein can bind directly. Plays a role in error-free postreplication repair (PRR) of damaged DNA and maintains genomic stability through acting as a ubiquitin ligase for 'Lys-63'-linked polyubiquitination of chromatin-bound PCNA (By similarity).

Catalytic activity

  • S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.
    EC:2.3.2.27 (UniProtKB | ENZYME | Rhea)

Pathway

Protein modification; protein ubiquitination.

Features

Showing features for binding site.

110031002003004005006007008009001,000
TypeIDPosition(s)Description
Binding site294-301ATP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular Componentnuclear matrix
Cellular Componentnucleolus
Cellular Componentnucleoplasm
Cellular ComponentRNA polymerase II transcription regulator complex
Molecular FunctionATP binding
Molecular FunctionATP-dependent activity, acting on DNA
Molecular FunctionATP-dependent chromatin remodeler activity
Molecular FunctionDNA binding
Molecular Functionhelicase activity
Molecular Functionhydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides
Molecular Functiontransferase activity
Molecular Functionubiquitin protein ligase binding
Molecular Functionzinc ion binding
Biological ProcessmRNA transcription by RNA polymerase II
Biological Processpositive regulation of transcription by RNA polymerase II
Biological Processprotein ubiquitination
Biological Processregulation of neurogenesis

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Helicase-like transcription factor
  • EC number
  • Alternative names
    • P113
    • RING-type E3 ubiquitin transferase HLTF
    • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 3
    • Sucrose nonfermenting protein 2-like 3
    • TNF-response element-binding protein

Gene names

    • Name
      Hltf
    • Synonyms
      Smarca3, Snf2l3, Zbu1

Organism names

  • Taxonomic identifier
  • Strain
    • C57BL/6J
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus

Accessions

  • Primary accession
    Q6PCN7
  • Secondary accessions
    • O35596
    • O35597
    • Q80VT6

Proteomes

Organism-specific databases

Subcellular Location

Cytoplasm
Nucleus
Nucleus, nucleolus
Nucleus, nucleoplasm
Note: Nuclear localization is stimulated by progesterone.

Keywords

PTM/Processing

Features

Showing features for chain, modified residue, cross-link.

TypeIDPosition(s)Description
ChainPRO_00000561851-1003Helicase-like transcription factor
Modified residue27Omega-N-methylarginine
Cross-link112Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)
Modified residue195Phosphotyrosine; by JAK2
Cross-link211Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)
Modified residue394Phosphoserine
Modified residue395Phosphoserine
Modified residue397Phosphoserine
Modified residue730Phosphothreonine

Keywords

Proteomic databases

PTM databases

Expression

Tissue specificity

Expressed in brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle.

Developmental stage

Expressed in the heart from 11.5 dpc. Gradually increases in skeletal muscle to 18.5 dpc.

Gene expression databases

Interaction

Subunit

Interacts with SP1 and SP3 independently of DNA; the interaction with these transcriptional factors may be required for basal transcription of target genes. Interacts with EGR1; the interaction requires prior binding to DNA and represses c-Rel via a DNA looping mechanism. Interacts with GATA4. Interacts with PCNA; the interaction promotes polyubiquitination of PCNA through association with the UBE2B-RAD18 and UBE2V2-UBE2N ubiquitin ligase complexes. Interacts with RAD18, SHPRH, UBE2V2 and UBE2N (By similarity).

Complex viewer

View interactors in UniProtKB
View CPX-899 in Complex Portal

Protein-protein interaction databases

Miscellaneous

Structure

Family & Domains

Features

Showing features for region, compositional bias, domain, motif, zinc finger.

TypeIDPosition(s)Description
Region38-287DNA-binding
Compositional bias317-355Basic and acidic residues
Region317-373Disordered
Compositional bias356-373Polar residues
Domain433-600Helicase ATP-binding
Motif551-554DEGH box
Zinc finger754-795RING-type
Domain831-990Helicase C-terminal
Region919-1003Interaction with SP1 and SP3

Sequence similarities

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    1,003
  • Mass (Da)
    113,317
  • Last updated
    2004-07-05 v1
  • Checksum
    91F08509ACEA5513
MSYTFTRGPVWKYSQSVQYGSHENIPRLSYSTFLPHFEFQDIIPPDDFLTSDEEQDLVLFGTMRGQVVGLRYYTGVVNNNEMVALQREPNNPYDKNAIKVNNVNGNQVGHIKREIAAAVAYIMDNKLAQVEGVVPFGASNTFTMPLYMTFWGKEENRNVVLEQLKKHGFKLGPTPKTLGSSLENAWGSGRAGPSYSRPAHVAVQMTTDQLKTEFDKLFEDLKEDDRTVEMEPAEAIETPLLPHQKQALAWMIARENSKELPPFWEQRNDLYYNTITNFSVKERPENVHGGILADDMGLGKTLTAIAVILTNFDDGRPLLSKRGKKNHPGKEYKDETIKRRGSNMDKKEDGHSESSTCGEEPSISGTPEKSSCTLSQLSSVCPKRRKISVQYIESSDSEEIETSELPQKMKGKLKNVQLNTKSRVKGSSKVKEDSKFALTFFASATQRKMLKKGMSMMECSEACDTGERTRATLIICPLSVLSNWIDQFGQHVKSEVHLNFYVYYGPDRIRDSAWLSKQDIILTTYNILTHDYGTKDDSPLHSIKWLRVILDEGHAIRNPNAQQTKAVLELEAERRWVLTGTPIQNSLKDLWSLLSFLKLKPFIDREWWYRIIQRPVTTGDEGGLRRLQSLIKNITLRRTKTSKIKGKPVLELPERKVFIQHITLSEEERKIYQSVKNEGKAAIGRYFTEGTVLAHYADVLGLLLRLRQICCHTHLLTNGMSSSGPSRSDTPEELRKMLIEKMKIILSSGSDEECAICLDSLTFPVITHCAHVFCKPCICQVIHSEQPHAKCPLCRNEIHGDNLLECPPEELACDSDKESSMEWKSSSKINALMHALIELRTKDPNIKSLVVSQFTTFLSLIETPLKASGFVFTRLDGSMAQKKRVESIQRFQNTEAGSPTIMLLSLKAGGVGLNLCAASRVFLMDPAWNPAAEDQCFDRCHRLGQKQEVIITKFIVKDSVEENMLKIQNTKRDLAAGAFGTKKTDANDMKQAKINEIRTLIDL

Computationally mapped potential isoform sequences

There are 2 potential isoforms mapped to this entry

View all
EntryEntry nameGene nameLength
E9PVX5E9PVX5_MOUSEHltf566
G3UVU1G3UVU1_MOUSEHltf628

Sequence caution

The sequence AAB63915.1 differs from that shown. Reason: Miscellaneous discrepancy Intron retention.

Features

Showing features for sequence conflict, compositional bias.

TypeIDPosition(s)Description
Sequence conflict150in Ref. 1; AAB64175/AAB63915
Sequence conflict191in Ref. 1; AAB64175/AAB63915
Compositional bias317-355Basic and acidic residues
Compositional bias356-373Polar residues
Sequence conflict394in Ref. 1; AAB64175/AAB63915
Sequence conflict648in Ref. 1; AAB64175/AAB63915
Sequence conflict902-905in Ref. 1; AAB64175
Sequence conflict918in Ref. 1; AAB64175
Sequence conflict980in Ref. 1; AAB64175

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AF010138
EMBL· GenBank· DDBJ
AAB64175.1
EMBL· GenBank· DDBJ
Genomic DNA
AF010600
EMBL· GenBank· DDBJ
AAB63915.1
EMBL· GenBank· DDBJ
mRNA Sequence problems.
BC039796
EMBL· GenBank· DDBJ
AAH39796.1
EMBL· GenBank· DDBJ
mRNA
BC057116
EMBL· GenBank· DDBJ
AAH57116.1
EMBL· GenBank· DDBJ
mRNA
BC059240
EMBL· GenBank· DDBJ
AAH59240.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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