Q6NZQ8 · MARH1_MOUSE

  • Protein
    E3 ubiquitin-protein ligase MARCHF1
  • Gene
    Marchf1
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

E3 ubiquitin-protein ligase that mediates ubiquitination of TFRC, CD86, FAS and MHC class II proteins, such as HLA-DR alpha and beta, and promotes their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. By constitutively ubiquitinating MHC class II proteins in immature dendritic cells, down-regulates their cell surface localization thus sequestering them in the intracellular endosomal system.

Catalytic activity

  • S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.
    EC:2.3.2.27 (UniProtKB | ENZYME | Rhea)

Pathway

Protein modification; protein ubiquitination.

Features

Showing features for binding site.

128920406080100120140160180200220240260280
TypeIDPosition(s)Description
Binding site80Zn2+ 1 (UniProtKB | ChEBI)
Binding site83Zn2+ 1 (UniProtKB | ChEBI)
Binding site97Zn2+ 2 (UniProtKB | ChEBI)
Binding site99Zn2+ 2 (UniProtKB | ChEBI)
Binding site107Zn2+ 1 (UniProtKB | ChEBI)
Binding site110Zn2+ 1 (UniProtKB | ChEBI)
Binding site123Zn2+ 2 (UniProtKB | ChEBI)
Binding site126Zn2+ 2 (UniProtKB | ChEBI)

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentcytoplasm
Cellular Componentearly endosome membrane
Cellular Componentendoplasmic reticulum membrane
Cellular Componentlate endosome membrane
Cellular Componentlysosomal membrane
Cellular Componentlysosome
Cellular Componentplasma membrane
Cellular Componenttrans-Golgi network membrane
Molecular FunctionMHC protein binding
Molecular Functionubiquitin protein ligase activity
Molecular Functionubiquitin-protein transferase activity
Molecular Functionzinc ion binding
Biological Processantigen processing and presentation of peptide antigen via MHC class II
Biological Processimmune response
Biological Processprotein polyubiquitination

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    E3 ubiquitin-protein ligase MARCHF1
  • EC number
  • Alternative names
    • Membrane-associated RING finger protein 1
    • Membrane-associated RING-CH protein I (MARCH-I)
    • RING-type E3 ubiquitin transferase MARCHF1

Gene names

    • Name
      Marchf1
    • Synonyms
      March1

Organism names

  • Taxonomic identifier
  • Strains
    • C57BL/6J
    • NOD
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus

Accessions

  • Primary accession
    Q6NZQ8
  • Secondary accessions
    • Q3U7M8
    • Q3UVF8
    • Q8C294
    • Q8CBA1

Proteomes

Organism-specific databases

Subcellular Location

Cytoplasmic vesicle membrane
; Multi-pass membrane protein
Late endosome membrane
; Multi-pass membrane protein
Early endosome membrane
; Multi-pass membrane protein
Golgi apparatus, trans-Golgi network membrane
; Multi-pass membrane protein
Lysosome membrane
; Multi-pass membrane protein

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane155-175Helical
Transmembrane197-217Helical

Keywords

Phenotypes & Variants

Disruption phenotype

Shows increase in the cell surface expression or half-life of MHC class II. Null cells have accumulated MHC class II and CD86 at the cell surface and a low antigen-presenting ability for exogenous antigens, in conventional dendritic cells (PubMed:19917682).
Null cells have high antigen-presenting ability for exogenous antigens in B-cells (PubMed:17255932).

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis114Loss of ubiquitin ligase activity and loss of down-regulation of CD86 cell surface expression.
Mutagenesis225Partial loss of down-regulation of CD86 cell surface expression. Almost complete loss of down-regulation of CD86 cell surface expression; when associated with F-232.
Mutagenesis232Almost complete loss of down-regulation of CD86 cell surface expression; when associated with S-225.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 7 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00002743661-289E3 ubiquitin-protein ligase MARCHF1

Post-translational modification

Has a short half-life. Instability/short half-life permits rapid changes that allow efficient induction of antigen presentation once antigen presenting cells, APCs, receive maturation signals. Small changes in protein levels significantly alter the cell surface display of MHC class II proteins.

Proteomic databases

PTM databases

Expression

Gene expression databases

Interaction

Protein-protein interaction databases

Miscellaneous

Structure

Family & Domains

Features

Showing features for region, compositional bias, zinc finger.

TypeIDPosition(s)Description
Region1-66Responsible for low stability
Region13-69Disordered
Compositional bias14-69Polar residues
Zinc finger72-133RING-CH-type
Region222-279Responsible for down-regulation of CD86 and MHC class II cell surface expression

Domain

The RING-CH-type zinc finger domain is required for E3 ligase activity.

Keywords

Phylogenomic databases

Family and domain databases

Sequence & Isoforms

Align isoforms (5)
  • Sequence status
    Complete

This entry describes 5 isoforms produced by Alternative splicing.

Q6NZQ8-1

This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

  • Length
    289
  • Mass (Da)
    32,347
  • Last updated
    2007-02-06 v2
  • Checksum
    0DBE9A308D5ED0CE
MLGWCEAIARNPHRIPNTTRTPETSGDVADASQTSTLNEKSPGRSASRSSNISKASSPTTGTAPRSQSRLSVCPSTQDICRICHCEGDEESPLITPCRCTGTLRFVHQSCLHQWIKSSDTRCCELCKYDFIMETKLKPLRKWEKLQMTTSERRKIFCSVTFHVIAVTCVVWSLYVLIDRTAEEIKQGNDNGVLEWPFWTKLVVVAIGFTGGLVFMYVQCKVYVQLWRRLKAYNRVIFVQNCPDTANKLEKNFPCNVNTEIKDAVVVPVPQTGSNTLPTAEGAPPEVIPV

Q6NZQ8-2

  • Name
    2
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical
    • 1-54: MLGWCEAIARNPHRIPNTTRTPETSGDVADASQTSTLNEKSPGRSASRSSNISK → MNLTMSNMTSSHICCNFLNMWKKSKISTMYYLNQDAKLSNLFLQ

Q6NZQ8-3

  • Name
    3
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical
    • 1-37: MLGWCEAIARNPHRIPNTTRTPETSGDVADASQTSTL → MPLHQISVIPARETASNGRSSMGRNKEKNKEVE

Q6NZQ8-4

  • Name
    4
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical
    • 1-54: MLGWCEAIARNPHRIPNTTRTPETSGDVADASQTSTLNEKSPGRSASRSSNISK → MNLTMSNMTSSHICCNFLNMWKKSKISTMYYLNQDAKLSNLFLQ
    • 187-190: Missing

Q6NZQ8-5

  • Name
    5
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical

Computationally mapped potential isoform sequences

There is 1 potential isoform mapped to this entry

View all
EntryEntry nameGene nameLength
D3YVY0D3YVY0_MOUSEMarchf1540

Features

Showing features for alternative sequence, compositional bias, sequence conflict.

TypeIDPosition(s)Description
Alternative sequenceVSP_0227271-37in isoform 3
Alternative sequenceVSP_0227261-54in isoform 2 and isoform 4
Compositional bias14-69Polar residues
Sequence conflict85in Ref. 1; BAE31341
Alternative sequenceVSP_022728187-190in isoform 4 and isoform 5

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AK036489
EMBL· GenBank· DDBJ
BAC29449.1
EMBL· GenBank· DDBJ
mRNA
AK089040
EMBL· GenBank· DDBJ
BAC40715.1
EMBL· GenBank· DDBJ
mRNA
AK137337
EMBL· GenBank· DDBJ
BAE23311.1
EMBL· GenBank· DDBJ
mRNA
AK152592
EMBL· GenBank· DDBJ
BAE31341.1
EMBL· GenBank· DDBJ
mRNA
AK163081
EMBL· GenBank· DDBJ
BAE37183.1
EMBL· GenBank· DDBJ
mRNA
BC066008
EMBL· GenBank· DDBJ
AAH66008.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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