Q6N4D3 · SYT_RHOPA
- ProteinThreonine--tRNA ligase
- GenethrS
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids658 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the attachment of threonine to tRNA(Thr) in a two-step reaction: L-threonine is first activated by ATP to form Thr-AMP and then transferred to the acceptor end of tRNA(Thr). Also edits incorrectly charged L-seryl-tRNA(Thr).
Catalytic activity
- tRNA(Thr) + L-threonine + ATP = L-threonyl-tRNA(Thr) + AMP + diphosphate + H+
Cofactor
Note: Binds 1 zinc ion per subunit.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 341 | Zn2+ (UniProtKB | ChEBI) | |||
Binding site | 392 | Zn2+ (UniProtKB | ChEBI) | |||
Binding site | 525 | Zn2+ (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | ATP binding | |
Molecular Function | metal ion binding | |
Molecular Function | threonine-tRNA ligase activity | |
Molecular Function | tRNA binding | |
Biological Process | threonyl-tRNA aminoacylation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameThreonine--tRNA ligase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Alphaproteobacteria > Hyphomicrobiales > Nitrobacteraceae > Rhodopseudomonas
Accessions
- Primary accessionQ6N4D3
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000101035 | 1-658 | Threonine--tRNA ligase | ||
Interaction
Structure
Family & Domains
Features
Showing features for domain, region.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 1-63 | TGS | |||
Region | 245-548 | Catalytic | |||
Sequence similarities
Belongs to the class-II aminoacyl-tRNA synthetase family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length658
- Mass (Da)74,110
- Last updated2004-07-05 v1
- MD5 ChecksumF2927B2504CB869577A5E61A35CB9734
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
BX572604 EMBL· GenBank· DDBJ | CAE28847.1 EMBL· GenBank· DDBJ | Genomic DNA |