Q6MG71 · CTL4_RAT
- ProteinCholine transporter-like protein 4
- GeneSlc44a4
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids707 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score4/5
Function
function
Choline transporter that plays a role in the choline-acetylcholine system and is required to the efferent innervation of hair cells in the olivocochlear bundle for the maintenance of physiological function of outer hair cells and the protection of hair cells from acoustic injury (By similarity).
Also described as a thiamine pyrophosphate transporter in colon, may mediate the absorption of microbiota-generated thiamine pyrophosphate and contribute to host thiamine (vitamin B1) homeostasis (By similarity).
Also described as a thiamine pyrophosphate transporter in colon, may mediate the absorption of microbiota-generated thiamine pyrophosphate and contribute to host thiamine (vitamin B1) homeostasis (By similarity).
Catalytic activity
- choline(out) + n H+(in) = choline(in) + n H+(out)
- thiamine diphosphate(out) = thiamine diphosphate(in)
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | apical plasma membrane | |
Cellular Component | plasma membrane | |
Molecular Function | antiporter activity | |
Molecular Function | choline transmembrane transporter activity | |
Molecular Function | thiamine pyrophosphate transmembrane transporter activity | |
Biological Process | acetylcholine biosynthetic process | |
Biological Process | acetylcholine secretion | |
Biological Process | choline transport | |
Biological Process | neuromast hair cell development | |
Biological Process | otolith formation | |
Biological Process | positive regulation of cell growth | |
Biological Process | thiamine pyrophosphate transmembrane transport |
Keywords
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameCholine transporter-like protein 4
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Rattus
Accessions
- Primary accessionQ6MG71
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Membrane ; Multi-pass membrane protein
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-32 | Cytoplasmic | ||||
Sequence: MGKKQKENEAYGNSAKYDPSFRGPIKNRGCTD | ||||||
Transmembrane | 33-53 | Helical | ||||
Sequence: IICCVLFLVFILGYIVVGLVA | ||||||
Topological domain | 54-227 | Extracellular | ||||
Sequence: WVYGDPRQVLYPRNSTGAYCGVGDNKDKPYVLYFNILSCAAAINVISIAENGLQCPTPQVCVSSCPQTPWVVEGFQLSNTVGDVYKEYRNFCVPAVSPDMVVMDSLQKGLCPSFLLPSTPALGRCFPLPNINFTLPEQLQINNTTVSKGISGLLDSINARDVSVKIFEDFAQSW | ||||||
Transmembrane | 228-248 | Helical | ||||
Sequence: YWILVALGVALVLSLLFILLL | ||||||
Topological domain | 249-250 | Cytoplasmic | ||||
Sequence: RL | ||||||
Transmembrane | 251-271 | Helical | ||||
Sequence: VAAPLVLLLIVGVLAVLAYGI | ||||||
Topological domain | 272-307 | Extracellular | ||||
Sequence: YHCWQQYRELRDQGVSITQLGFTANLSAYQNVKETW | ||||||
Transmembrane | 308-328 | Helical | ||||
Sequence: LAALIILAVLEGVLLLMLIFL | ||||||
Topological domain | 329-356 | Cytoplasmic | ||||
Sequence: RQRIRIAIALLKEASRAVGQMMSTMFYP | ||||||
Transmembrane | 357-377 | Helical | ||||
Sequence: LVTFVLLVICIGYWAVTALYL | ||||||
Topological domain | 378-452 | Extracellular | ||||
Sequence: ATSGQPQYVYWVHNTSTPGCEKVLVNVSCDPMAPLNSSCPELKCTFTGYSSSGLAQRSLFNLQIYGILGLFWTVN | ||||||
Transmembrane | 453-473 | Helical | ||||
Sequence: WVLALGQCVLAGAFASFYWAF | ||||||
Topological domain | 474-498 | Cytoplasmic | ||||
Sequence: HKPRDIPTFPLSSAFIRTLRYHTGS | ||||||
Transmembrane | 499-519 | Helical | ||||
Sequence: LAFGALILTLVQIARVILEYI | ||||||
Topological domain | 520-557 | Extracellular | ||||
Sequence: DHKLRGSQNPVARCIICCFKCCLWCLEKFIKFLNRNAY | ||||||
Transmembrane | 558-578 | Helical | ||||
Sequence: IMIAIYGKNFCVSAKNAFMLL | ||||||
Topological domain | 579-594 | Cytoplasmic | ||||
Sequence: MRNVVRVVVLDKVTDL | ||||||
Transmembrane | 595-615 | Helical | ||||
Sequence: LLFFGKLLVVGGVGVLSFFFF | ||||||
Topological domain | 616-635 | Extracellular | ||||
Sequence: SGRIKGLGKDFKNPDLNYYW | ||||||
Transmembrane | 636-656 | Helical | ||||
Sequence: LPIMTSIMGAYVIASGFFSVF | ||||||
Topological domain | 657-707 | Cytoplasmic | ||||
Sequence: GMCVDTLFLCFLEDLERNDGSQERPYYMPKALLKILGKKNEVPTGGKNRKK |
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain, glycosylation.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000191725 | 1-707 | Choline transporter-like protein 4 | |||
Sequence: MGKKQKENEAYGNSAKYDPSFRGPIKNRGCTDIICCVLFLVFILGYIVVGLVAWVYGDPRQVLYPRNSTGAYCGVGDNKDKPYVLYFNILSCAAAINVISIAENGLQCPTPQVCVSSCPQTPWVVEGFQLSNTVGDVYKEYRNFCVPAVSPDMVVMDSLQKGLCPSFLLPSTPALGRCFPLPNINFTLPEQLQINNTTVSKGISGLLDSINARDVSVKIFEDFAQSWYWILVALGVALVLSLLFILLLRLVAAPLVLLLIVGVLAVLAYGIYHCWQQYRELRDQGVSITQLGFTANLSAYQNVKETWLAALIILAVLEGVLLLMLIFLRQRIRIAIALLKEASRAVGQMMSTMFYPLVTFVLLVICIGYWAVTALYLATSGQPQYVYWVHNTSTPGCEKVLVNVSCDPMAPLNSSCPELKCTFTGYSSSGLAQRSLFNLQIYGILGLFWTVNWVLALGQCVLAGAFASFYWAFHKPRDIPTFPLSSAFIRTLRYHTGSLAFGALILTLVQIARVILEYIDHKLRGSQNPVARCIICCFKCCLWCLEKFIKFLNRNAYIMIAIYGKNFCVSAKNAFMLLMRNVVRVVVLDKVTDLLLFFGKLLVVGGVGVLSFFFFSGRIKGLGKDFKNPDLNYYWLPIMTSIMGAYVIASGFFSVFGMCVDTLFLCFLEDLERNDGSQERPYYMPKALLKILGKKNEVPTGGKNRKK | ||||||
Glycosylation | 67 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 185 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 195 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 196 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 296 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 391 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 403 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 413 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N |
Post-translational modification
N-glycosylated; N-glycosylation of Asn-67 and Asn-391 is required for a proper thiamine pyrophosphate uptake.
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
Highly expressed in intestine, kidney and stomach. Also expressed in testis and lung.
Gene expression databases
Interaction
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length707
- Mass (Da)78,737
- Last updated2004-07-05 v1
- ChecksumB37416E597969501
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A8I5ZM85 | A0A8I5ZM85_RAT | Slc44a4 | 656 |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
BX883045 EMBL· GenBank· DDBJ | CAE83975.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BC079178 EMBL· GenBank· DDBJ | AAH79178.1 EMBL· GenBank· DDBJ | mRNA |