Q6LXQ1 · FDHA1_METMP
- ProteinF420-dependent formate dehydrogenase 1 subunit alpha
- GenefdhA1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids674 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Catalyzes the oxidation of formate to carbon dioxide, with coenzyme F420 as the electron acceptor (PubMed:18791018).
In vitro can also use methyl viologen as electron acceptor (PubMed:18791018).
In vitro can also use methyl viologen as electron acceptor (PubMed:18791018).
Miscellaneous
The genome of M.maripaludis harbors two sets of genes encoding the F420-dependent formate dehydrogenase (Fdh), fdhA1-fdhB1 (Fdh1 isozyme) and fdhA2-fdhB2 (Fdh2 isozyme) (PubMed:18791018).
The Fdh1 isozyme is the primary Fdh and the Fdh2 isozyme may not play a major role (PubMed:18791018).
The Fdh1 isozyme is the primary Fdh and the Fdh2 isozyme may not play a major role (PubMed:18791018).
Catalytic activity
- oxidized coenzyme F420-(gamma-L-Glu)(n) + formate + 2 H+ = reduced coenzyme F420-(gamma-L-Glu)(n) + CO2
oxidized coenzyme F420-(γ-L-Glu)(n) RHEA-COMP:12939 + CHEBI:15740 + 2 CHEBI:15378 = reduced coenzyme F420-(γ-L-Glu)(n) RHEA-COMP:14378 + CHEBI:16526
Cofactor
Protein has several cofactor binding sites:
Note: Binds 1 [4Fe-4S] cluster.
Note: Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 10 | [4Fe-4S] cluster (UniProtKB | ChEBI) | |||
Binding site | 13 | [4Fe-4S] cluster (UniProtKB | ChEBI) | |||
Binding site | 17 | [4Fe-4S] cluster (UniProtKB | ChEBI) | |||
Binding site | 45 | [4Fe-4S] cluster (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | 4 iron, 4 sulfur cluster binding | |
Molecular Function | formate dehydrogenase (NAD+) activity | |
Molecular Function | metal ion binding | |
Molecular Function | molybdopterin cofactor binding | |
Biological Process | formate metabolic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameF420-dependent formate dehydrogenase 1 subunit alpha
- EC number
- Short namesFdh1 subunit alpha
Gene names
Organism names
- Strain
- Taxonomic lineageArchaea > Euryarchaeota > Methanomada group > Methanococci > Methanococcales > Methanococcaceae > Methanococcus
Accessions
- Primary accessionQ6LXQ1
Proteomes
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000461127 | 1-674 | F420-dependent formate dehydrogenase 1 subunit alpha | ||
Interaction
Subunit
Dimer of an alpha (FdhA1) and a beta (FdhB1) subunit.
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 3-59 | 4Fe-4S Mo/W bis-MGD-type | |||
Sequence similarities
Belongs to the prokaryotic molybdopterin-containing oxidoreductase family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length674
- Mass (Da)74,225
- Last updated2011-05-03 v1
- Checksum9DAB8BE5FCEDA186
Features
Showing features for non-standard residue.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Non-standard residue | 132 | Selenocysteine | |||
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
BX950229 EMBL· GenBank· DDBJ | CAF30854.1 EMBL· GenBank· DDBJ | Genomic DNA |