Q6L743 · DOIAD_STRKN
- Protein2-deoxy-scyllo-inosamine dehydrogenase
- GenekanE
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids343 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the oxidation of 2-deoxy-scyllo-inosamine (DOIA) with NAD+ or NADP+, forming 3-amino-2,3-dideoxy-scyllo-inosose (amino-DOI).
Catalytic activity
- 2-deoxy-scyllo-inosamine + NADP+ = 3-amino-2,3-dideoxy-scyllo-inosose + NADPH + H+
Cofactor
Note: Binds 2 Zn2+ ions per subunit.
Pathway
Metabolic intermediate biosynthesis; 2-deoxystreptamine biosynthesis; 2-deoxystreptamine from D-glucose 6-phosphate: step 3/4.
Antibiotic biosynthesis; kanamycin biosynthesis.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 37 | Zn2+ 1 (UniProtKB | ChEBI); catalytic | ||||
Sequence: C | ||||||
Binding site | 59 | Zn2+ 1 (UniProtKB | ChEBI); catalytic | ||||
Sequence: H | ||||||
Binding site | 91 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 94 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 97 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 105 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 146 | Zn2+ 1 (UniProtKB | ChEBI); catalytic | ||||
Sequence: E |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | metal ion binding | |
Molecular Function | oxidoreductase activity | |
Biological Process | antibiotic biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended name2-deoxy-scyllo-inosamine dehydrogenase
- EC number
- Short namesDOIA dehydrogenase
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageBacteria > Actinomycetota > Actinomycetes > Kitasatosporales > Streptomycetaceae > Streptomyces
Accessions
- Primary accessionQ6L743
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000234044 | 1-343 | 2-deoxy-scyllo-inosamine dehydrogenase | |||
Sequence: MKALVFHSPEKATFEQRDVPTPRPGEALVHIAYNSICGSDLSLYRGVWHGFGYPVVPGHEWSGTVVEINGANGHDQSLVGKNVVGDLTCACGNCAACGRGTPVLCENLQELGFTKDGACAEYMTIPVDNLRPLPDALSLRSACQVEPLAVALNAVSIAGVAPGDRVAVMGAGGIGLMLMQVARHLGGEVTVVSEPVAERRAVAGQLGATELCSAEPGQLAELVARRPELTPDVVLEASGYPAALQEAIEVVRPGGRIGLIGYRVEETGPMSPQHIAVKALTLRGSLGPGGRFDDAVELLAKGDDIAVEPLLSHEFGLADYATALDLALSRTNGNVRSFFNLRD |
Structure
Family & Domains
Sequence similarities
Belongs to the zinc-containing alcohol dehydrogenase family. DOIA dehydrogenase subfamily.
Family and domain databases
Sequence
- Sequence statusComplete
- Length343
- Mass (Da)35,936
- Last updated2004-07-05 v1
- ChecksumA61AFA1521BAEFB8
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AJ582817 EMBL· GenBank· DDBJ | CAF60530.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AB164642 EMBL· GenBank· DDBJ | BAD20754.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AJ628422 EMBL· GenBank· DDBJ | CAF31584.1 EMBL· GenBank· DDBJ | Genomic DNA |