Q6L6S1 · EGCSE_HYDVU

Function

function

Hydrolysis of the glycosidic linkage between oligosaccharides and ceramides of glycosphingolipids, optimal substrates appear to be the glycosphingolipids with a gangliotetraose structure.

Catalytic activity

Activity regulation

Cu2+, zinc, manganese, calcium, magnesium and EDTA have no significant effects on enzyme activity. Enzyme requires presence of detergents such as Triton X-100 and Lubrol PX for the hydrolysis of glycosphingolipids. Taurodeoxycholate strongly inhibits the enzyme activity.

pH Dependence

Optimum pH is 3.0-4.0. Highly stable at acidic pH.

Features

Showing features for active site.

TypeIDPosition(s)Description
Active site230Proton donor

GO annotations

AspectTerm
Cellular Componentextracellular region
Molecular Functionendoglycosylceramidase activity
Biological Processcellulose catabolic process
Biological Processglycosphingolipid catabolic process

Keywords

Enzyme and pathway databases

Protein family/group databases

    • GH5Glycoside Hydrolase Family 5

Names & Taxonomy

Protein names

  • Recommended name
    Endoglycoceramidase
  • EC number
  • Short names
    EGCase
  • Alternative names
    • Glycosphingolipid-specific enzyme
      (GSL-specific enzyme
      )

Organism names

Accessions

  • Primary accession
    Q6L6S1

Proteomes

Subcellular Location

Secreted
Note: Released from digestive cells into the gastric cavity, and eventually into the surrounding medium, during the digestive process.

Keywords

PTM/Processing

Features

Showing features for signal, chain, glycosylation.

TypeIDPosition(s)Description
Signal1-17
ChainPRO_000039038218-517Endoglycoceramidase
Glycosylation99N-linked (GlcNAc...) asparagine
Glycosylation298N-linked (GlcNAc...) asparagine
Glycosylation380N-linked (GlcNAc...) asparagine
Glycosylation393N-linked (GlcNAc...) asparagine

Keywords

Expression

Tissue specificity

Expressed uniformly in digestive cells, tentacles and peduncle regions suggesting expression in the endoderm throughout the whole body (at protein level).

Structure

Family & Domains

Sequence similarities

Belongs to the glycosyl hydrolase 5 (cellulase A) family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    517
  • Mass (Da)
    59,685
  • Last updated
    2004-07-05 v1
  • Checksum
    9042532367D263F4
MISVALIILFLAKVISGKSDDFISVNPETNMLIDGYGRERFFHGTNVVVKHFPFHPETTGFNKDTFSEDDMKILQKFGLNSIRLGMMLPGYVPKREEYNETYIKVIQSIVTTAAKYGIYTLLDMHQDVFSPKFCVEGMPDWIVNTQGAKDFPMPLHKPFNLDPKTGYPYPEDCAKFSWADYYFTEAAGQAFQNLYDNVDGLRDEWAQFWKKTADVFKEEPSVIGYELINEPFCGNVFKHPTLLIPGVADYLNLQPTYDALQKAIRQVDEEHNIFFEGVTWDFFEVGFTEVPGGKQYQNRSVLSYHYYEPPDFSKKLNFEARLLDLKRLKCGGFLTEMFTVGTDFNSMFEMFDLCDKFKQSWHGWMYKSYGCIEQNLGCLNMSSPGKESIQIANTSRTYPQAVAGRTQSYAFDIKTKVFTLVYETVGSCKSGRTIVYFNKNLHYPNGYRYEINPNFKVTPSENEYFLYLDEVNKVPNTVVTFKLFPLSFTDSEDIHPVTVMGDKHLSENHNENEKKKK

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AB179748
EMBL· GenBank· DDBJ
BAD20464.1
EMBL· GenBank· DDBJ
mRNA

Similar Proteins

Disclaimer

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