Q6L5H6 · P2C50_ORYSJ
- ProteinProtein phosphatase 2C 50
- GenePP2C50
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids387 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Protein phosphatase involved in abscisic acid (ABA) signaling. Together with PYL3 and SAPK10, may form an ABA signaling module involved in stress response.
Catalytic activity
- H2O + O-phospho-L-seryl-[protein] = L-seryl-[protein] + phosphate
Cofactor
Mn2+ (UniProtKB | Rhea| CHEBI:29035 )
Note: Binds 2 magnesium or manganese ions per subunit.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | metal ion binding | |
Molecular Function | myosin phosphatase activity | |
Molecular Function | protein serine/threonine phosphatase activity | |
Biological Process | abscisic acid-activated signaling pathway |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameProtein phosphatase 2C 50
- EC number
- Short namesOsPP2C50
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > Liliopsida > Poales > Poaceae > BOP clade > Oryzoideae > Oryzeae > Oryzinae > Oryza > Oryza sativa
Accessions
- Primary accessionQ6L5H6
- Secondary accessions
Proteomes
Genome annotation databases
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 265 | Decreases binding affinity to PYL3 15-fold; when associated with M-267. | ||||
Sequence: S → F | ||||||
Mutagenesis | 265 | Abolishes interaction with PYR/PYL/RCAR abscisic acid intracellular receptors; when associated with K-267. | ||||
Sequence: S → K | ||||||
Mutagenesis | 267 | Abolishes interaction with PYR/PYL/RCAR abscisic acid intracellular receptors; when associated with K-265. | ||||
Sequence: I → K | ||||||
Mutagenesis | 267 | Decreases binding affinity to PYL3 15-fold; when associated with F-265. | ||||
Sequence: I → M |
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000363297 | 1-387 | Protein phosphatase 2C 50 | |||
Sequence: MAAAAAAAAICGEDETAARVGCTGEWAGGIERVDLGERKEAVAAAGAGKRSVYLMDCAPVWGCASTRGRSAEMEDASAAVPRFADVPVRLLASRRDLDALGLDADALRLPAHLFGVFDGHGGAEVANYCRERIHVVLSEELKRLGKNLGEMGEVDMKEHWDDVFTKCFQRVDDEVSGRVTRVVNGGGEVRSEPVTAENVGSTAVVALVCSSHVVVANCGDSRIVLCRGKEPVALSIDHKPDRKDERARIEAQGGKVIQWNGYRVSGILAMSRSIGDRYLKPFVIPKPEVMVVPRAKDDDCLILASDGLWDVVSNEEACKVARRQILLWHKNNGAASPLSDEGEGSTDPAAQAAADYLMRLALKKGSEDNITVIVVDLKPRKKLKNIS |
Proteomic databases
Expression
Induction
Induced by abscisic acid (ABA).
Interaction
Subunit
Interacts with PYL3, PYL5, PYL9 and PYL10. Binding to PYL3, PYL5, PYL9 and PYL10 is dependent on the presence of abscisic acid (ABA). Interacts with SAPK10.
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain, motif.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 60-377 | PPM-type phosphatase | ||||
Sequence: VWGCASTRGRSAEMEDASAAVPRFADVPVRLLASRRDLDALGLDADALRLPAHLFGVFDGHGGAEVANYCRERIHVVLSEELKRLGKNLGEMGEVDMKEHWDDVFTKCFQRVDDEVSGRVTRVVNGGGEVRSEPVTAENVGSTAVVALVCSSHVVVANCGDSRIVLCRGKEPVALSIDHKPDRKDERARIEAQGGKVIQWNGYRVSGILAMSRSIGDRYLKPFVIPKPEVMVVPRAKDDDCLILASDGLWDVVSNEEACKVARRQILLWHKNNGAASPLSDEGEGSTDPAAQAAADYLMRLALKKGSEDNITVIVVDL | ||||||
Motif | 264-268 | Modulates binding affinity to PYR/PYL/RCAR abscisic acid intracellular receptors | ||||
Sequence: VSGIL |
Sequence similarities
Belongs to the PP2C family.
Phylogenomic databases
Family and domain databases
Sequence & Isoform
- Sequence statusComplete
This entry describes 2 isoforms produced by Alternative splicing.
Q6L5H6-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- Length387
- Mass (Da)41,554
- Last updated2004-07-05 v1
- Checksum8DE5BAF0E1CCA2E6
Q6L5H6-2
- Name2
Features
Showing features for alternative sequence.
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AC097112 EMBL· GenBank· DDBJ | AAT39223.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AC119291 EMBL· GenBank· DDBJ | AAV59393.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AP008211 EMBL· GenBank· DDBJ | BAF18083.2 EMBL· GenBank· DDBJ | Genomic DNA | ||
AP014961 EMBL· GenBank· DDBJ | BAS95091.1 EMBL· GenBank· DDBJ | Genomic DNA |