Q6J5K9 · ARMI_DROME
- ProteinProbable RNA helicase armi
- Genearmi
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids1188 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Probable RNA helicase required for axial polarization of the oocyte during early and mid oogenesis (PubMed:15035984).
Plays a central role in RNA interference (RNAi) process, a process that mediates mRNA destruction of translational repression (PubMed:15035984).
Required for the assembly of the RISC complex, a complex required for target RNA destruction or repression (PubMed:15035985).
May be required in the RISC assembly to unwind miRNAs, in the production of single-stranded miRNA from the double-stranded miRNA, a key step in RISC formation (PubMed:15035985).
Required both for the translational control of oskar (osk) mRNA and cytoskeletal polarization in the oocyte (PubMed:15035984).
Required for somatic primary piRNA biogenesis (PubMed:20966047).
Involved in repression of long interspersed nuclear elements (LINEs) including HeT-A, I-element and TART LINEs (PubMed:17428915).
Plays a central role in RNA interference (RNAi) process, a process that mediates mRNA destruction of translational repression (PubMed:15035984).
Required for the assembly of the RISC complex, a complex required for target RNA destruction or repression (PubMed:15035985).
May be required in the RISC assembly to unwind miRNAs, in the production of single-stranded miRNA from the double-stranded miRNA, a key step in RISC formation (PubMed:15035985).
Required both for the translational control of oskar (osk) mRNA and cytoskeletal polarization in the oocyte (PubMed:15035984).
Required for somatic primary piRNA biogenesis (PubMed:20966047).
Involved in repression of long interspersed nuclear elements (LINEs) including HeT-A, I-element and TART LINEs (PubMed:17428915).
Catalytic activity
- ATP + H2O = ADP + H+ + phosphate
Features
Showing features for binding site.
GO annotations
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameProbable RNA helicase armi
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Metazoa > Ecdysozoa > Arthropoda > Hexapoda > Insecta > Pterygota > Neoptera > Endopterygota > Diptera > Brachycera > Muscomorpha > Ephydroidea > Drosophilidae > Drosophila > Sophophora
Accessions
- Primary accessionQ6J5K9
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Note: Component of Yb bodies, an electron dense structure containing Yb protein found in somatic cells of ovary and testis.
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000080708 | 1-1188 | Probable RNA helicase armi | |||
Sequence: MFTYVSKFFTNPDRNREDILESLDRENSFLDQKLMEEKMDQQLKANPNEINGVLSNKIAELTHGLSEMDVSKESSCTARKGVITSLDGDRGVIDKDVLFETKVAEDIILDLHVGCVVEYLTFTTGEAMRVVKVKSILEHSWEDTSQKEIEKAVDNLKNEKPTFFNTETRSVLGLISQRLASSIDVETEYGQLTVELDNIEMNFIPTNGDRVRLECNIQLDDGFVDKQGEILEVTKLFPTRIQEGEKCIVERVYVHMVVLGPETYILKTDLPTGTDLHLGDIVLADLIECQYSKFTRRAIKITPLEKNFGATKLTQQSSMGSSGSGKAVTVTGVNRFITAELWQKESVSLKLTNNLNRTLRLESITVCNDSESQLSVVSPLESKEISSGSEITVTFEIHTQFLGEAIEKYVLNFDLLKVRRVFTVIVCKTKEEVAEAEKRMIAAEALMAPGRNSQERSRFYANQVWCNKVDVIPGQQIVTKRRFVALRLGCFEVPKELRQICLTSERRQEMIKAIEQHYSFLTEPLSIKTYMHRFRLLLHLEEIECFVNFRNYDRDRAHFLRDGEFLTLQIENLAERRPSLVIGDTLRVINPWSDPDSQTTKSYEGIIHKVLFDRILLKFHSSFQEKYNGEDYRLEFYFSRYSFRKQHHAISKIVGVMGEDFLFPSKVTKRENPQLDVYMKDDDMYLYDSKLEWYNQSLNSIQKRAVFNILRGEAENIPYVLFGPPGSGKTMTLIETLLQLVRNLPGARILVGTPSNSSADLVTKRLIDSKALLQGDFIRLVSYNQVEKDLIPPEIMSYCATSDVGAVGSCEDKMMVTESGLKLRCQAKFIGTHRITISTCTTLGNFLQLGFPAGHFTHVLFDEAGQCTEPETMVPIVMLTKKRSQVVLSGDPRQLQSIVTSRIASKMGFSISFLERLLERSPYRKDLQRFPESSGYNPLVLTKLLYNYRALPSIMSIYSRLFYDDELIPVLSEKDSRESRLLSKLRCVFESEKDIPQAHGTFFYGIIGENRQNNDSPSWFNPQEVREVFLMTIALYRANVTADQIGIITPYQKQVKMLRSMFIGTDVVMPKIGSVEEFQGQERDIILISTVRSSEEILRMDARFSLGFVRCSKRLNVAVSRARAMMIIFGNPHLLAVDECWRQLILFCVKNNAYFGCDLPQMVINQKDEVPVCLETFVPSLNTTDDLN |
Proteomic databases
Expression
Tissue specificity
Abundant in oocytes and syncytial blastoderm. Expressed at low level throughout development, including somatic tissues. First apparent early in oogenesis, in the cytoplasm of stem cells and mitotically dividing cystoblasts. In regions 2a and 2b of the germarium, it is most concentrated in the center of the germline cysts, where the pro-oocyte is located. In stage 1 and early stage 2 egg chambers, it accumulates at the anterior of the oocyte, near the ring canals. It also extends through the ring canals forming a branched structure that links the early oocyte with adjacent nurse cells. In stage 3 cysts, it accumulates at the posterior cortex and localizes to extensions that pass through the oocyte into the nurse cells. Through stages 4 to 7, it continues to be somewhat enriched at the posterior cortex of the oocyte, but at significantly lower level. In stage 9 to 10 egg chambers, it is found throughout the cytoplasm of the oocyte and nurse cells, with slight enrichment at the oocyte cortex.
Gene expression databases
Interaction
Subunit
Forms a complex with piwi and fs1Yb; this interaction is required for proper piRNA loading and nuclear localization of piwi. The interaction of piwi and fs1Yb is likely to occur via armi.
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | Q6J5K9 | fs(1)Yb Q9W4W2 | 4 | EBI-2890374, EBI-3424083 | |
BINARY | Q6J5K9 | piwi Q9VKM1 | 4 | EBI-2890374, EBI-3406276 |
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for motif.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Motif | 862-865 | DEAG box | ||||
Sequence: DEAG |
Sequence similarities
Belongs to the DNA2/NAM7 helicase family. SDE3 subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,188
- Mass (Da)135,658
- Last updated2014-07-09 v4
- ChecksumBA1D8F4305825B0A
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
M9NDG7 | M9NDG7_DROME | armi | 1154 |
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 522 | in Ref. 1; AAT12000 | ||||
Sequence: T → K | ||||||
Sequence conflict | 527 | in Ref. 1; AAT12000 | ||||
Sequence: I → V | ||||||
Sequence conflict | 905 | in Ref. 1; AAT12000 | ||||
Sequence: S → L |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AY598469 EMBL· GenBank· DDBJ | AAT12000.1 EMBL· GenBank· DDBJ | mRNA | ||
AE014296 EMBL· GenBank· DDBJ | AAF47775.2 EMBL· GenBank· DDBJ | Genomic DNA | ||
AE014296 EMBL· GenBank· DDBJ | AAX52729.1 EMBL· GenBank· DDBJ | Genomic DNA |