Q6GNL7 · AL1L1_XENLA
- ProteinCytosolic 10-formyltetrahydrofolate dehydrogenase
- Genealdh1l1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids902 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score4/5
Function
function
Cytosolic 10-formyltetrahydrofolate dehydrogenase that catalyzes the NADP+-dependent conversion of 10-formyltetrahydrofolate to tetrahydrofolate and carbon dioxide. May also have an NADP+-dependent aldehyde dehydrogenase activity towards formaldehyde, acetaldehyde, propionaldehyde, and benzaldehyde.
Catalytic activity
- (6R)-10-formyltetrahydrofolate + NADP+ + H2O = (6S)-5,6,7,8-tetrahydrofolate + CO2 + NADPH + H+This reaction proceeds in the forward direction.
Features
Showing features for binding site, active site, site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 88-90 | (6R)-10-formyltetrahydrofolate (UniProtKB | ChEBI) | |||
Active site | 106 | Proton donor | |||
Binding site | 142 | (6R)-10-formyltetrahydrofolate (UniProtKB | ChEBI) | |||
Site | 142 | Essential for catalytic activity | |||
Binding site | 571-573 | NADP+ (UniProtKB | ChEBI) | |||
Binding site | 597-600 | NADP+ (UniProtKB | ChEBI) | |||
Binding site | 630-635 | NADP+ (UniProtKB | ChEBI) | |||
Binding site | 650-651 | NADP+ (UniProtKB | ChEBI) | |||
Active site | 673 | Proton acceptor | |||
Binding site | 673-674 | NADP+ (UniProtKB | ChEBI) | |||
Active site | 707 | Proton donor | |||
Binding site | 757 | NADP+ (UniProtKB | ChEBI) | |||
Binding site | 804-806 | NADP+ (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | aldehyde dehydrogenase (NAD+) activity | |
Molecular Function | formyltetrahydrofolate dehydrogenase activity | |
Biological Process | 10-formyltetrahydrofolate catabolic process | |
Biological Process | biosynthetic process | |
Biological Process | NADPH regeneration | |
Biological Process | one-carbon metabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameCytosolic 10-formyltetrahydrofolate dehydrogenase
- EC number
- Short names10-FTHFDH; FDH
- Alternative names
Gene names
Organism names
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Amphibia > Batrachia > Anura > Pipoidea > Pipidae > Xenopodinae > Xenopus > Xenopus
Accessions
- Primary accessionQ6GNL7
Proteomes
Organism-specific databases
Subcellular Location
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000315999 | 1-902 | Cytosolic 10-formyltetrahydrofolate dehydrogenase | ||
Modified residue | 354 | O-(pantetheine 4'-phosphoryl)serine | |||
Post-translational modification
Phosphopantetheinylation at Ser-354 by AASDHPPT is required for the formyltetrahydrofolate dehydrogenase activity.
Keywords
- PTM
Expression
Gene expression databases
Interaction
Subunit
Homotetramer.
Structure
Family & Domains
Features
Showing features for region, domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Region | 1-310 | Hydrolase domain | |||
Domain | 318-395 | Carrier | |||
Region | 417-902 | Aldehyde dehydrogenase domain | |||
Domain
The N-terminal hydrolase domain has an NADP-independent formyltetrahydrofolate hydrolase activity, releasing formate and tetrahydrofolate.
The C-terminal aldehyde dehydrogenase domain has an NADP-dependent dehydrogenase activity. It catalyzes the oxidation of formate, released by the hydrolysis of formyltetrahydrofolate, into CO2.
The carrier domain is phosphopantetheinylated and uses the 4'-phosphopantetheine/4'-PP swinging arm to transfer the formyl group released by the N-terminal formyltetrahydrofolate hydrolase activity to the C-terminal aldehyde dehydrogenase domain that catalyzes its NADP-dependent oxidation into CO2. The overall NADP-dependent physiological reaction requires the 3 domains (N-terminal hydrolase, C-terminal aldehyde dehydrogenase and carrier domains) to convert formyltetrahydrofolate into tetrahydrofolate and CO2.
Sequence similarities
In the N-terminal section; belongs to the GART family.
In the C-terminal section; belongs to the aldehyde dehydrogenase family. ALDH1L subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length902
- Mass (Da)99,982
- Last updated2004-07-19 v1
- ChecksumC484728096D4C281
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
BC073490 EMBL· GenBank· DDBJ | AAH73490.1 EMBL· GenBank· DDBJ | mRNA |