Q6GMV2 · SMYD5_HUMAN
- ProteinHistone-lysine N-trimethyltransferase SMYD5
- GeneSMYD5
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids418 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Histone methyltransferase that specifically trimethylates 'Lys-20' of histone H4 to form trimethylated histone H4 lysine 20 (H4K20me3) which represents a specific tag for epigenetic transcriptional repression (By similarity).
In association with the NCoR corepressor complex, is involved in the repression of toll-like receptor 4 (TLR4)-target inflammatory genes in macrophages by catalyzing the formation of H4K20me3 at the gene promoters (By similarity).
Plays an important role in embryonic stem (ES) cell self-renewal and differentiation (By similarity).
Promotes ES cell maintenance by silencing differentiation genes through deposition of H4K20me3 marks (By similarity).
Maintains genome stability of ES cells during differentiation through regulation of heterochromatin formation and repression of endogenous repetitive DNA elements by depositing H4K20me3 marks (PubMed:28951459).
In association with the NCoR corepressor complex, is involved in the repression of toll-like receptor 4 (TLR4)-target inflammatory genes in macrophages by catalyzing the formation of H4K20me3 at the gene promoters (By similarity).
Plays an important role in embryonic stem (ES) cell self-renewal and differentiation (By similarity).
Promotes ES cell maintenance by silencing differentiation genes through deposition of H4K20me3 marks (By similarity).
Maintains genome stability of ES cells during differentiation through regulation of heterochromatin formation and repression of endogenous repetitive DNA elements by depositing H4K20me3 marks (PubMed:28951459).
Catalytic activity
- L-lysyl20-[histone H4] + 3 S-adenosyl-L-methionine = 3 H+ + N6,N6,N6-trimethyl-L-lysyl20-[histone H4] + 3 S-adenosyl-L-homocysteine
RHEA-COMP:15554 CHEBI:29969 Position: 20+ 3 CHEBI:59789 = 3 CHEBI:15378 + RHEA-COMP:15998 CHEBI:61961 Position: 20+ 3 CHEBI:57856
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | histone H4K20 methyltransferase activity | |
Molecular Function | histone H4K20 trimethyltransferase activity | |
Molecular Function | metal ion binding | |
Biological Process | methylation | |
Biological Process | negative regulation of gene expression, epigenetic | |
Biological Process | regulation of stem cell differentiation | |
Biological Process | regulation of stem cell division | |
Biological Process | retrotransposon silencing by heterochromatin formation |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameHistone-lysine N-trimethyltransferase SMYD5
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Primates > Haplorrhini > Catarrhini > Hominidae > Homo
Accessions
- Primary accessionQ6GMV2
- Secondary accessions
Proteomes
Organism-specific databases
Disease & Variants
Variants
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The viewer provides 446 variants from UniProt as well as other sources including ClinVar and dbSNP.
Organism-specific databases
Miscellaneous
Genetic variation databases
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000227788 | 1-418 | Histone-lysine N-trimethyltransferase SMYD5 | |||
Sequence: MAASMCDVFSFCVGVAGRARVSVEVRFVSSAKGKGLFATQLIRKGETIFVERPLVAAQFLWNALYRYRACDHCLRALEKAEENAQRLTGKPGQVLPHPELCTVRKDLHQNCPHCQVMYCSAECRLAATEQYHQVLCPGPSQDDPLHPLNKLQEAWRSIHYPPETASIMLMARMVATVKQAKDKDRWIRLFSQFCNKTANEEEEIVHKLLGDKFKGQLELLRRLFTEALYEEAVSQWFTPDGFRSLFALVGTNGQGIGTSSLSQWVHACDTLELKPQDREQLDAFIDQLYKDIEAATGEFLNCEGSGLFVLQSCCNHSCVPNAETSFPENNFLLHVTALEDIKPGEEICISYLDCCQRERSRHSRHKILRENYLFVCSCPKCLAEADEPNVTSEEEEEEEEEEEGEPEDAELGDEMTDV |
Proteomic databases
PTM databases
Expression
Interaction
Subunit
Interacts with the N-CoR complex (By similarity).
Interacts with EHMT2 and CBX5 (By similarity).
Interacts with EHMT2 and CBX5 (By similarity).
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
XENO | Q6GMV2 | Ppargc1a O70343 | 4 | EBI-11057552, EBI-1371053 | |
BINARY | Q6GMV2 | ZNF417 Q8TAU3 | 3 | EBI-11057552, EBI-740727 | |
BINARY | Q6GMV2 | ZNF587 Q96SQ5 | 3 | EBI-11057552, EBI-6427977 |
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for domain, zinc finger, region, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 21-352 | SET | ||||
Sequence: VSVEVRFVSSAKGKGLFATQLIRKGETIFVERPLVAAQFLWNALYRYRACDHCLRALEKAEENAQRLTGKPGQVLPHPELCTVRKDLHQNCPHCQVMYCSAECRLAATEQYHQVLCPGPSQDDPLHPLNKLQEAWRSIHYPPETASIMLMARMVATVKQAKDKDRWIRLFSQFCNKTANEEEEIVHKLLGDKFKGQLELLRRLFTEALYEEAVSQWFTPDGFRSLFALVGTNGQGIGTSSLSQWVHACDTLELKPQDREQLDAFIDQLYKDIEAATGEFLNCEGSGLFVLQSCCNHSCVPNAETSFPENNFLLHVTALEDIKPGEEICISYL | ||||||
Zinc finger | 98-136 | MYND-type | ||||
Sequence: PELCTVRKDLHQNCPHCQVMYCSAECRLAATEQYHQVLC | ||||||
Region | 385-418 | Disordered | ||||
Sequence: ADEPNVTSEEEEEEEEEEEGEPEDAELGDEMTDV | ||||||
Compositional bias | 388-418 | Acidic residues | ||||
Sequence: PNVTSEEEEEEEEEEEGEPEDAELGDEMTDV |
Sequence similarities
Belongs to the class V-like SAM-binding methyltransferase superfamily.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length418
- Mass (Da)47,341
- Last updated2006-03-21 v2
- Checksum11073818FF5EEB83
Computationally mapped potential isoform sequences
There are 3 potential isoforms mapped to this entry
Sequence caution
Features
Showing features for sequence conflict, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 7 | in Ref. 3; AAB38131 | ||||
Sequence: D → A | ||||||
Sequence conflict | 280-281 | in Ref. 3; AAB38131 | ||||
Sequence: QL → HV | ||||||
Compositional bias | 388-418 | Acidic residues | ||||
Sequence: PNVTSEEEEEEEEEEEGEPEDAELGDEMTDV |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CH471053 EMBL· GenBank· DDBJ | EAW99744.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CH471053 EMBL· GenBank· DDBJ | EAW99745.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BC073806 EMBL· GenBank· DDBJ | AAH73806.1 EMBL· GenBank· DDBJ | mRNA | Different initiation | |
U50383 EMBL· GenBank· DDBJ | AAB38131.1 EMBL· GenBank· DDBJ | mRNA |