Q6FRH8 · VPS15_CANGA

Function

function

Serine/threonine-protein kinase that plays a role in signaling in modulation of host immune response, intracellular survival and virulence (PubMed:25223215).
Required for impediment of phagosomal maturation in THP-1 macrophages (PubMed:25223215).
Regulatory subunit of the autophagy-specific VPS34 PI3-kinase complex I essential to recruit the ATG8-phosphatidylinositol conjugate and the ATG12-ATG5 conjugate to the pre-autophagosomal structure (By similarity).
Within the PS34 PI3-kinase complex I, VPS15-mediated phosphorylation of VPS34 may be required for recruiting VPS34 to the membrane but not for activation of its PI3K activity (PubMed:25223215).
Is also involved in endosome-to-Golgi retrograde transport as part of the VPS34 PI3-kinase complex II (By similarity).
This second complex is required for the endosome-to-Golgi retrieval of PEP1 and KEX2, and the recruitment of VPS5 and VPS7, two components of the retromer complex, to endosomal membranes (probably through the synthesis of a specific pool of phosphatidylinositol 3-phosphate recruiting the retromer to the endosomes) (By similarity).
By regulating VPS34 kinase activity, VPS15 appears to be essential for the efficient delivery of soluble hydrolases to the yeast vacuole (By similarity).

Catalytic activity

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site33-41ATP (UniProtKB | ChEBI)
Binding site54ATP (UniProtKB | ChEBI)
Active site147Proton acceptor

GO annotations

AspectTerm
Cellular Componentendosome membrane
Cellular ComponentGolgi apparatus
Cellular Componentlate endosome
Cellular Componentnucleus-vacuole junction
Cellular Componentphosphatidylinositol 3-kinase complex, class III, type I
Cellular Componentphosphatidylinositol 3-kinase complex, class III, type II
Cellular Componentvacuole-isolation membrane contact site
Molecular FunctionATP binding
Molecular Functionhistone H2AS1 kinase activity
Molecular Functionubiquitin binding
Biological Processcellular response to potassium ion starvation
Biological Processlate endosome to vacuole transport
Biological Processpexophagy
Biological Processphosphatidylinositol phosphate biosynthetic process
Biological Processpositive regulation of transcription elongation by RNA polymerase II
Biological Processprotein localization to vacuole
Biological Processprotein phosphorylation
Biological Processprotein retention in Golgi apparatus
Biological Processprotein targeting to vacuole
Biological Processvacuole inheritance

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    non-specific serine/threonine protein kinase
  • EC number

Gene names

    • Name
      VPS15
    • Ordered locus names
      CAGL0H08437g

Organism names

Accessions

  • Primary accession
    Q6FRH8

Proteomes

Organism-specific databases

Phenotypes & Variants

Disruption phenotype

Leads to large vacuoles and defective respiratory growth (PubMed:25223215).
Blocks maturation of the phagosomes and renders cells hyperadherent to epithelial cells and susceptible to the antimicrobial arsenal of primary murine and cultured human macrophages, as well as to thermal, salt, oxidative, genotoxic, cell wall and cell membrane stresses (PubMed:25223215).
Displays defects in protein trafficking (PubMed:25223215).

Miscellaneous

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004588941-1410non-specific serine/threonine protein kinase

Post-translational modification

Autophosphorylated.

Keywords

Interaction

Subunit

Component of the autophagy-specific VPS34 PI3-kinase complex I composed of VPS15, VPS30, VPS34, ATG14 and ATG38; and of the VPS34 PI3-kinase complex II composed of VPS15, VPS30, VPS34 and VPS38.

Protein-protein interaction databases

Structure

3D structure databases

Family & Domains

Features

Showing features for domain, repeat.

TypeIDPosition(s)Description
Domain27-299Protein kinase
Repeat441-478HEAT 1
Repeat485-525HEAT 2
Repeat556-594HEAT 3
Repeat596-633HEAT 4
Repeat635-672HEAT 5
Repeat1037-1076WD 1
Repeat1187-1226WD 2
Repeat1230-1273WD 3

Sequence similarities

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    1,410
  • Mass (Da)
    161,501
  • Last updated
    2004-07-19 v1
  • Checksum
    8FCA4E775FCF85E2
MGGQLSLLAQTAPSIGIFSYIDILDETHYVSQLNNSRFLKTCKALDPNGEIVVKVFIKPKEDSSLEKIIQRLKREAVLLSALPNVLNYSKIFESTRCGYLVRQHLKTNLYDRLSTRPYFTEIETKFVVFQLLQALKDIHDLDVIHGDIKTENLLLTSWDWVVLSDFCSNIKPAYIPDDNPGEFSFYFDTSKRRACYLAPEKFDSAKASGDGVHQATKEMDIFSLGCCIAELYLDGAALFNLSQLFKYKSGDYTLNDILPQTISKSSPVLKDILQDMLQVDPKKRLSAHELLEKYRTIYFPDTFYDFLYDYCKTLVTLGTSVPCADQIEVNTTLQDHLGSIDEILIKIYCDFGRICKSLKLPVLDQEDMNYNTKDSFLRVSDKLLRLENYETDKITASIQNEVSLIILVFLCKEFRNLQFPENKVKALQLILAFSLFVLDDTKLDRTLPYLVAALEDDSTRVKVMAMNCVTTLIKEVKHPNQLNENIFVDYLLPRVQALLQNGQEESLVRVAIASNLSDLALKANLFQEYCHTMQSSTIPNIVHDFESIEVIRKYSRKLQQLFEDLTVSILTDPEISVKVALLKNILPLCKYFGREKTNDVILSHLITYLNDRDPALRMYLVECISGIAILLGPITMEQYILPLIIQTITDEEELVVVSVLKNLKDLLKTRFVNKKYFYDITKFLSPLILHPNSWIRNFVLTTLVECINQMSKAEVYCVLYPVLRPFFDFDVDFTGDMLISCAKLPVSRNTYNLLRSWNNRSKKTFFWQRVTTNYVDAFGNSTINFVDKRYVKENYGLKTMKVESNIHLHTNENENIPLTLEDKFWIDKFKNSGLTDNELWKIVALREYVVRSSRSSSKKPETVTSIVSKLSLTPSNFSIENVMPNTVFFDIEFLHPETLTFNDLDVTNTNSIKESESNTFTDNHSKVIEMKGSLIFKTPRIPTTLSNLKNIYVQLEPTNNHSEGHAHLSARNQPANFIVKSSYEGQDKIIEKYLKQLNILPSLKEYKEFGFVSENTTAEADVINLHGKFVRSYPQIFDGTLLQSEVLLGTKSFMIYGSDQGALTVWDIDRLANEKSITRPLYYECSAEITCIKGLSGYDSFCVGLKSSEILIFRISLSKNGKAKNLQELICIRSLNLIGENSSEYPIQIECCPNNDQFQLVVLSNYSNVYLFDIRTMKVIEKLELNADYGCTISMVLDDKNNLLFFGTVSGIIEMWDARYFVQIRAWTFGESLPINKLAIMEQENKSLLVVCGGVDSAFFTLWNIEKLSCKHVFVRSNEQPSLDSFNVIDADKLDKLAFEKNNSNIKPIVQIFNNKVLYMDDIGRLLHILDTRNPEKSSTFAGSKVELHSFSVLQVTASLTMSLQKHNYQKEVNNSNYTSSRVMTVNVFQLKNKPYMLLTDEEGYINIYT

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CR380954
EMBL· GenBank· DDBJ
CAG60099.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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