Q6DJK5 · Q6DJK5_XENLA
- ProteinCytosolic non-specific dipeptidase
- Genecndp2.L
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids474 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score4/5
Function
function
Catalyzes the peptide bond hydrolysis in dipeptides, displaying a non-redundant activity toward threonyl dipeptides. Mediates threonyl dipeptide catabolism in a tissue-specific way. Has high dipeptidase activity toward cysteinylglycine, an intermediate metabolite in glutathione metabolism. Metabolizes N-lactoyl-amino acids, both through hydrolysis to form lactic acid and amino acids, as well as through their formation by reverse proteolysis. Plays a role in the regulation of cell cycle arrest and apoptosis.
Catalytic activity
- (S)-lactate + L-phenylalanine = N-[(S)-lactoyl]-L-phenylalanine + H2OThis reaction proceeds in the forward and the backward directions.
- L-cysteinylglycine + H2O = L-cysteine + glycineThis reaction proceeds in the forward direction.
- L-seryl-L-threonine + H2O = L-threonine + L-serineThis reaction proceeds in the forward direction.
- L-threonyl-L-serine + H2O = L-threonine + L-serineThis reaction proceeds in the forward direction.
- L-threonyl-L-threonine + H2O = 2 L-threonineThis reaction proceeds in the forward direction.
Cofactor
Note: Binds 2 manganese ions per subunit.
Features
Showing features for binding site, active site, site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 99 | Mn2+ 2 (UniProtKB | ChEBI) | |||
Active site | 101 | ||||
Binding site | 132 | Mn2+ 2 (UniProtKB | ChEBI) | |||
Binding site | 132 | Mn2+ 1 (UniProtKB | ChEBI) | |||
Active site | 166 | Proton acceptor | |||
Binding site | 167 | Mn2+ 1 (UniProtKB | ChEBI) | |||
Binding site | 195 | Mn2+ 2 (UniProtKB | ChEBI) | |||
Binding site | 195 | substrate; ligand shared between homodimeric partners; in other chain | |||
Binding site | 228 | substrate; ligand shared between homodimeric partners | |||
Site | 228 | Important for catalytic activity | |||
Binding site | 330 | substrate; ligand shared between homodimeric partners | |||
Binding site | 343 | substrate; ligand shared between homodimeric partners; in other chain | |||
Binding site | 417 | substrate; ligand shared between homodimeric partners; in other chain | |||
Binding site | 445 | Mn2+ 1 (UniProtKB | ChEBI) | |||
Binding site | 445 | substrate; ligand shared between homodimeric partners; in other chain | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | carboxypeptidase activity | |
Molecular Function | metal ion binding | |
Molecular Function | metallodipeptidase activity | |
Biological Process | proteolysis |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameCytosolic non-specific dipeptidase
- EC number
- Alternative names
Gene names
Organism names
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Amphibia > Batrachia > Anura > Pipoidea > Pipidae > Xenopodinae > Xenopus > Xenopus
Accessions
- Primary accessionQ6DJK5
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
PTM/Processing
Keywords
- PTM
Expression
Gene expression databases
Interaction
Subunit
Homodimer.
Structure
Sequence
- Sequence statusComplete
- Length474
- Mass (Da)52,841
- Last updated2004-08-16 v1
- ChecksumF97784E8FB36CB78
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
BC075171 EMBL· GenBank· DDBJ | AAH75171.1 EMBL· GenBank· DDBJ | mRNA | ||
BC157410 EMBL· GenBank· DDBJ | AAI57411.1 EMBL· GenBank· DDBJ | mRNA |