Q6BPU5 · DPH1_DEBHA
- Protein2-(3-amino-3-carboxypropyl)histidine synthase subunit 1
- GeneDPH1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids420 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the first step of diphthamide biosynthesis, a post-translational modification of histidine which occurs in elongation factor 2. DPH1 and DPH2 transfer a 3-amino-3-carboxypropyl (ACP) group from S-adenosyl-L-methionine (SAM) to a histidine residue, the reaction is assisted by a reduction system comprising DPH3 and a NADH-dependent reductase, predominantly CBR1.
Catalytic activity
- L-histidyl-[translation elongation factor 2] + S-adenosyl-L-methionine = 2-[(3S)-amino-3-carboxypropyl]-L-histidyl-[translation elongation factor 2] + S-methyl-5'-thioadenosine + H+
Cofactor
Note: Binds 1 [4Fe-4S] cluster per subunit. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.
Pathway
Protein modification; peptidyl-diphthamide biosynthesis.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 127 | [4Fe-4S] cluster (UniProtKB | ChEBI) | |||
Binding site | 233 | [4Fe-4S] cluster (UniProtKB | ChEBI) | |||
Binding site | 362 | [4Fe-4S] cluster (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | 2-(3-amino-3-carboxypropyl)histidine synthase complex | |
Cellular Component | cytoplasm | |
Molecular Function | 2-(3-amino-3-carboxypropyl)histidine synthase activity | |
Molecular Function | 4 iron, 4 sulfur cluster binding | |
Molecular Function | metal ion binding | |
Biological Process | protein histidyl modification to diphthamide |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended name2-(3-amino-3-carboxypropyl)histidine synthase subunit 1
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Saccharomycotina > Saccharomycetes > Saccharomycetales > Debaryomycetaceae > Debaryomyces
Accessions
- Primary accessionQ6BPU5
Proteomes
Organism-specific databases
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000083372 | 1-420 | 2-(3-amino-3-carboxypropyl)histidine synthase subunit 1 | ||
Interaction
Subunit
Component of the 2-(3-amino-3-carboxypropyl)histidine synthase complex composed of DPH1, DPH2, DPH3 and a NADH-dependent reductase, predominantly CBR1.
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length420
- Mass (Da)47,904
- Last updated2008-12-16 v2
- Checksum8E8D16525C7A8AAE
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CR382137 EMBL· GenBank· DDBJ | CAG88014.2 EMBL· GenBank· DDBJ | Genomic DNA |