Q6BG38 · Q6BG38_PARTE

  • Protein
    Adenylyltransferase and sulfurtransferase MOCS3 homolog
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Plays a central role in 2-thiolation of mcm5S2U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Acts by mediating the C-terminal thiocarboxylation of the sulfur carrier URM1. Its N-terminus first activates URM1 as acyl-adenylate (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 to form thiocarboxylation (-COSH) of its C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards URM1. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor; NFS1 probably acting as a sulfur donor for thiocarboxylation reactions.

Cofactor

Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Note: Binds 1 zinc ion per subunit.

Pathway

tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.

Features

Showing features for binding site, active site.

140950100150200250300350400
Type
IDPosition(s)Description
Binding site74ATP (UniProtKB | ChEBI)
Binding site95ATP (UniProtKB | ChEBI)
Binding site102-106ATP (UniProtKB | ChEBI)
Binding site119ATP (UniProtKB | ChEBI)
Binding site163-164ATP (UniProtKB | ChEBI)
Binding site203Zn2+ (UniProtKB | ChEBI)
Binding site206Zn2+ (UniProtKB | ChEBI)
Active site218Glycyl thioester intermediate; for adenylyltransferase activity
Binding site275Zn2+ (UniProtKB | ChEBI)
Binding site278Zn2+ (UniProtKB | ChEBI)
Active site368Cysteine persulfide intermediate; for sulfurtransferase activity

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular Componentcytosol
Molecular Functionadenylyltransferase activity
Molecular FunctionATP binding
Molecular Functionmetal ion binding
Molecular Functionnucleotidyltransferase activity
Molecular Functionthiosulfate sulfurtransferase activity
Molecular FunctionURM1 activating enzyme activity
Biological ProcesstRNA wobble position uridine thiolation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Adenylyltransferase and sulfurtransferase MOCS3 homolog
  • Alternative names
    • UBA4 homolog
    • Ubiquitin-like protein activator 4 homolog

Including 2 domains:

  • Recommended name
    Adenylyltransferase
  • EC number
  • Recommended name
    Sulfurtransferase
  • EC number

Gene names

    • ORF names
      GSPATT00000337001
      , PTMB.185

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • Stock d4-2
  • Taxonomic lineage
    Eukaryota > Sar > Alveolata > Ciliophora > Intramacronucleata > Oligohymenophorea > Peniculida > Parameciidae > Paramecium

Accessions

  • Primary accession
    Q6BG38

Proteomes

    • Identifier
    • Component
      Chromosome, Partially assembled WGS sequence

Subcellular Location

Keywords

Interaction

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain320-407Rhodanese

Sequence similarities

In the N-terminal section; belongs to the HesA/MoeB/ThiF family. UBA4 subfamily.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    409
  • Mass (Da)
    46,394
  • Last updated
    2004-08-16 v1
  • Checksum
    FD4B69B2FBFCFEA9
MKEYIEYLQNLLKQNKIEFEQEEDFIQHKVSQINNEFGYEHINRYKRQMILSEIGLTGQKQIHLAKVLIVGAGGIGAPAIYYLAGAGVGTIGLVDGDSVDVSNLHRQIIHNNYRQGMNKCESAKLQINEFNPLVNVITYKHHLSSANAIEIFQNYDVILDATDNPATRYLINDTAIYLNKPLVSGSSVGWEGQITVYGMQGPCYRCLFPQCPKTVQNCNEAGVFGVMPGLIGLIEALQAIKIIIGQQTLSQKMILIDGLRDVYKVVKLRGQQKDCIACQKQIKINDYDYASFAQTICSSSIPYRGGYKEIEWKDFLQIQRSEKVALLDVRPSSQYNIIKLDGFTNLPYSQIDQLQVEEYKDKEIYIMCRRGNNSRLACEYLKDKTPNIYNIIGGIDLYAKLYDPKMPLL

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CR548612
EMBL· GenBank· DDBJ
CAH03382.1
EMBL· GenBank· DDBJ
Genomic DNA
CT867985
EMBL· GenBank· DDBJ
CAK55902.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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