Q6BG38 · Q6BG38_PARTE
- ProteinAdenylyltransferase and sulfurtransferase MOCS3 homolog
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids409 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Plays a central role in 2-thiolation of mcm5S2U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Acts by mediating the C-terminal thiocarboxylation of the sulfur carrier URM1. Its N-terminus first activates URM1 as acyl-adenylate (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 to form thiocarboxylation (-COSH) of its C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards URM1. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor; NFS1 probably acting as a sulfur donor for thiocarboxylation reactions.
Cofactor
Note: Binds 1 zinc ion per subunit.
Pathway
tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 74 | ATP (UniProtKB | ChEBI) | |||
Binding site | 95 | ATP (UniProtKB | ChEBI) | |||
Binding site | 102-106 | ATP (UniProtKB | ChEBI) | |||
Binding site | 119 | ATP (UniProtKB | ChEBI) | |||
Binding site | 163-164 | ATP (UniProtKB | ChEBI) | |||
Binding site | 203 | Zn2+ (UniProtKB | ChEBI) | |||
Binding site | 206 | Zn2+ (UniProtKB | ChEBI) | |||
Active site | 218 | Glycyl thioester intermediate; for adenylyltransferase activity | |||
Binding site | 275 | Zn2+ (UniProtKB | ChEBI) | |||
Binding site | 278 | Zn2+ (UniProtKB | ChEBI) | |||
Active site | 368 | Cysteine persulfide intermediate; for sulfurtransferase activity | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | cytosol | |
Molecular Function | adenylyltransferase activity | |
Molecular Function | ATP binding | |
Molecular Function | metal ion binding | |
Molecular Function | nucleotidyltransferase activity | |
Molecular Function | thiosulfate sulfurtransferase activity | |
Molecular Function | URM1 activating enzyme activity | |
Biological Process | tRNA wobble position uridine thiolation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameAdenylyltransferase and sulfurtransferase MOCS3 homolog
- Alternative names
Including 2 domains:
- Recommended nameAdenylyltransferase
- EC number
- Recommended nameSulfurtransferase
- EC number
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Sar > Alveolata > Ciliophora > Intramacronucleata > Oligohymenophorea > Peniculida > Parameciidae > Paramecium
Accessions
- Primary accessionQ6BG38
Proteomes
Subcellular Location
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 320-407 | Rhodanese | |||
Sequence similarities
In the N-terminal section; belongs to the HesA/MoeB/ThiF family. UBA4 subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length409
- Mass (Da)46,394
- Last updated2004-08-16 v1
- ChecksumFD4B69B2FBFCFEA9
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CR548612 EMBL· GenBank· DDBJ | CAH03382.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CT867985 EMBL· GenBank· DDBJ | CAK55902.1 EMBL· GenBank· DDBJ | Genomic DNA |