Q6BCY4 · NB5R2_HUMAN
- ProteinNADH-cytochrome b5 reductase 2
- GeneCYB5R2
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids276 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
NADH-cytochrome b5 reductases are involved in desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction (By similarity).
Responsible for NADH-dependent lucigenin chemiluminescence in spermatozoa by reducing both lucigenin and 2-[4-iodophenyl]-3-[4-nitrophenyl]-5-[2,4-disulfophenyl]-2H tetrazolium monosodium salt (WST-1)
Responsible for NADH-dependent lucigenin chemiluminescence in spermatozoa by reducing both lucigenin and 2-[4-iodophenyl]-3-[4-nitrophenyl]-5-[2,4-disulfophenyl]-2H tetrazolium monosodium salt (WST-1)
Catalytic activity
- 2 Fe(III)-[cytochrome b5] + NADH = 2 Fe(II)-[cytochrome b5] + H+ + NAD+
Cofactor
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | endoplasmic reticulum membrane | |
Cellular Component | membrane | |
Cellular Component | mitochondrion | |
Cellular Component | nucleus | |
Molecular Function | cytochrome-b5 reductase activity, acting on NAD(P)H | |
Molecular Function | FAD binding | |
Biological Process | bicarbonate transport | |
Biological Process | sterol biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameNADH-cytochrome b5 reductase 2
- EC number
- Short namesb5R.2
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Primates > Haplorrhini > Catarrhini > Hominidae > Homo
Accessions
- Primary accessionQ6BCY4
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Disease & Variants
Features
Showing features for natural variant.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Natural variant | VAR_032321 | 15 | in dbSNP:rs11041525 | |||
Sequence: E → A | ||||||
Natural variant | VAR_032322 | 209 | in dbSNP:rs12801394 | |||
Sequence: N → D |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 449 variants from UniProt as well as other sources including ClinVar and dbSNP.
Organism-specific databases
Miscellaneous
Genetic variation databases
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000287548 | 1-276 | NADH-cytochrome b5 reductase 2 | |||
Sequence: MNSRRREPITLQDPEAKYPLPLIEKEKISHNTRRFRFGLPSPDHVLGLPVGNYVQLLAKIDNELVVRAYTPVSSDDDRGFVDLIIKIYFKNVHPQYPEGGKMTQYLENMKIGETIFFRGPRGRLFYHGPGNLGIRPDQTSEPKKTLADHLGMIAGGTGITPMLQLIRHITKDPSDRTRMSLIFANQTEEDILVRKELEEIARTHPDQFNLWYTLDRPPIGWKYSSGFVTADMIKEHLPPPAKSTLILVCGPPPLIQTAAHPNLEKLGYTQDMIFTY | ||||||
Modified residue | 17 | N6-acetyllysine | ||||
Sequence: K | ||||||
Modified residue | 18 | Phosphotyrosine | ||||
Sequence: Y |
Keywords
- PTM
Proteomic databases
2D gel databases
PTM databases
Expression
Tissue specificity
Restricted expression.
Gene expression databases
Organism-specific databases
Interaction
Binary interactions
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 15-127 | FAD-binding FR-type | ||||
Sequence: EAKYPLPLIEKEKISHNTRRFRFGLPSPDHVLGLPVGNYVQLLAKIDNELVVRAYTPVSSDDDRGFVDLIIKIYFKNVHPQYPEGGKMTQYLENMKIGETIFFRGPRGRLFYH |
Sequence similarities
Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.
Phylogenomic databases
Family and domain databases
Sequence & Isoform
- Sequence statusComplete
This entry describes 2 isoforms produced by Alternative splicing.
Q6BCY4-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- Length276
- Mass (Da)31,458
- Last updated2004-09-13 v1
- ChecksumA475039D1E56ABDA
Q6BCY4-2
- Name2
- Differences from canonical
- 221-276: WKYSSGFVTADMIKEHLPPPAKSTLILVCGPPPLIQTAAHPNLEKLGYTQDMIFTY → PWSAEGATLLSNSAQFH
Computationally mapped potential isoform sequences
There are 4 potential isoforms mapped to this entry
Features
Showing features for sequence conflict, alternative sequence.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 130 | in Ref. 1; AAF04811 | ||||
Sequence: G → R | ||||||
Alternative sequence | VSP_025559 | 221-276 | in isoform 2 | |||
Sequence: WKYSSGFVTADMIKEHLPPPAKSTLILVCGPPPLIQTAAHPNLEKLGYTQDMIFTY → PWSAEGATLLSNSAQFH | ||||||
Sequence conflict | 253 | in Ref. 1; AAF04811 | ||||
Sequence: P → T |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF169802 EMBL· GenBank· DDBJ | AAF04811.1 EMBL· GenBank· DDBJ | mRNA | ||
AY665398 EMBL· GenBank· DDBJ | AAT75296.1 EMBL· GenBank· DDBJ | mRNA | ||
BC001346 EMBL· GenBank· DDBJ | AAH01346.1 EMBL· GenBank· DDBJ | mRNA | ||
AL133582 EMBL· GenBank· DDBJ | CAB63726.1 EMBL· GenBank· DDBJ | mRNA |