Q6B7M7 · COF1_SHEEP

  • Protein
    Cofilin-1
  • Gene
    CFL1
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at transcript level
  • Annotation score
    5/5

Function

function

Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity (By similarity).
Important for normal progress through mitosis and normal cytokinesis (By similarity).
In conjunction with the subcortical maternal complex (SCMC), plays an essential role for zygotes to progress beyond the first embryonic cell divisions via regulation of actin dynamics (By similarity).
Required for the centralization of the mitotic spindle and symmetric division of zygotes (By similarity).
Plays a role in the regulation of cell morphology and cytoskeletal organization in epithelial cells (By similarity).
Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation (By similarity).
Required for neural tube morphogenesis and neural crest cell migration (By similarity).

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentactin cortical patch
Cellular Componentfocal adhesion
Cellular Componentgrowth cone
Cellular Componentlamellipodium
Cellular Componentlamellipodium membrane
Cellular Componentnuclear matrix
Cellular Componentruffle membrane
Molecular Functionactin filament binding
Biological Processactin filament fragmentation
Biological Processactin filament organization
Biological Processactin filament severing
Biological Processcytoskeleton organization
Biological Processestablishment of spindle localization
Biological Processmitotic cytokinesis
Biological Processpositive regulation by host of viral process
Biological Processpositive regulation of embryonic development
Biological Processregulation of cell morphogenesis
Biological Processregulation of dendritic spine morphogenesis
Biological Processresponse to virus

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Cofilin-1
  • Alternative names
    • Cofilin, non-muscle isoform

Gene names

    • Name
      CFL1

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Laurasiatheria > Artiodactyla > Ruminantia > Pecora > Bovidae > Caprinae > Ovis

Accessions

  • Primary accession
    Q6B7M7

Proteomes

Subcellular Location

Nucleus matrix
Cell projection, ruffle membrane
; Peripheral membrane protein
Cell projection, lamellipodium membrane
; Peripheral membrane protein
Cell projection, axon
Note: Colocalizes with the actin cytoskeleton in membrane ruffles and lamellipodia. Detected at the cleavage furrow and contractile ring during cytokinesis. Almost completely in nucleus in cells exposed to heat shock or 10% dimethyl sulfoxide.

Keywords

PTM/Processing

Features

Showing features for initiator methionine, modified residue, chain, cross-link.

TypeIDPosition(s)Description
Initiator methionine1Removed
Modified residue2N-acetylalanine
ChainPRO_00002149032-166Cofilin-1
Modified residue3Phosphoserine
Modified residue8Phosphoserine
Modified residue13N6-acetyllysine
Modified residue25Phosphothreonine
Modified residue41Phosphoserine
Modified residue68Phosphotyrosine
Modified residue73N6-acetyllysine
Cross-link132Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)
Modified residue140Phosphotyrosine
Modified residue144N6-acetyllysine
Modified residue156Phosphoserine

Post-translational modification

Inactivated by phosphorylation on Ser-3. Phosphorylated on Ser-3 in resting cells (By similarity).
Dephosphorylated by PDXP/chronophin; this restores its activity in promoting actin filament depolymerization. The phosphorylation of Ser-24 may prevent recognition of the nuclear localization signal (By similarity).
Phosphorylated via a ARRB1-RAC1-LIMK1-PAK1 cascade upon active ligand stimulation of atypical chemokine receptor ACKR2 (By similarity).

Keywords

Proteomic databases

Interaction

Subunit

Can bind G- and F-actin in a 1:1 ratio of cofilin to actin (By similarity).
It is a major component of intranuclear and cytoplasmic actin rods (By similarity).
Interacts with the subcortical maternal complex (SCMC) via interaction with TLE6 and NLRP5 (By similarity).
Interacts with C9orf72 (By similarity).

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for domain, motif.

TypeIDPosition(s)Description
Domain4-153ADF-H
Motif30-34Nuclear localization signal

Sequence similarities

Belongs to the actin-binding proteins ADF family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    166
  • Mass (Da)
    18,519
  • Last updated
    2007-01-23 v3
  • Checksum
    589EE8EC1ED12719
MASGVAVSDGVIKVFNDMKVRKSSTPEEVKKRKKAVLFCLSEDKKNIILEEGKEILVGDVGQTVDDPYATFVKMLPDKDCRYALYDATYETKESKKEDLVFIFWAPECAPLKSKMIYASSKDAIKKKLTGIKHELQANCYEEVKDRCTLAEKLGGSAVISLEGKPL

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AY676116
EMBL· GenBank· DDBJ
AAT77679.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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