Q6AYH1 · Q6AYH1_RAT
- ProteinPoly(A) polymerase
- GenePapolb
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids642 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score3/5
Function
function
Polymerase that creates the 3'-poly(A) tail of mRNA's.
Catalytic activity
- ATP + RNA(n) = diphosphate + RNA(n)-3'-adenine ribonucleotide
Cofactor
Protein has several cofactor binding sites:
Note: Binds 2 magnesium ions. Also active with manganese.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 101-103 | ATP (UniProtKB | ChEBI) | ||||
Sequence: FGS | ||||||
Binding site | 110 | ATP (UniProtKB | ChEBI) | ||||
Sequence: T | ||||||
Binding site | 114 | Mg2+ 1 (UniProtKB | ChEBI); catalytic | ||||
Sequence: D | ||||||
Binding site | 114 | Mg2+ 2 (UniProtKB | ChEBI); catalytic | ||||
Sequence: D | ||||||
Binding site | 114-116 | ATP (UniProtKB | ChEBI) | ||||
Sequence: DID | ||||||
Binding site | 116 | Mg2+ 1 (UniProtKB | ChEBI); catalytic | ||||
Sequence: D | ||||||
Binding site | 116 | Mg2+ 2 (UniProtKB | ChEBI); catalytic | ||||
Sequence: D | ||||||
Binding site | 168 | ATP (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 168 | Mg2+ 2 (UniProtKB | ChEBI); catalytic | ||||
Sequence: D | ||||||
Binding site | 229 | ATP (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 238 | ATP (UniProtKB | ChEBI) | ||||
Sequence: Y | ||||||
Binding site | 247-248 | ATP (UniProtKB | ChEBI) | ||||
Sequence: GV |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | nucleus | |
Molecular Function | ATP binding | |
Molecular Function | metal ion binding | |
Molecular Function | poly(A) RNA polymerase activity | |
Molecular Function | RNA binding | |
Biological Process | mRNA processing | |
Biological Process | RNA 3'-end processing |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePoly(A) polymerase
- EC number
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Rattus
Accessions
- Primary accessionQ6AYH1
Proteomes
Organism-specific databases
Subcellular Location
Structure
Family & Domains
Features
Showing features for compositional bias, region, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 1-19 | Polar residues | ||||
Sequence: MMPFAVTTQGAQQPAPAPK | ||||||
Region | 1-23 | Disordered | ||||
Sequence: MMPFAVTTQGAQQPAPAPKQYGI | ||||||
Domain | 22-215 | Poly(A) polymerase nucleotidyltransferase | ||||
Sequence: GISSPISLAAPKDTDRELTQKLIETLQPFGVFEEEEELQRRILILQKLNNLVKEWIREISESRNLPQAVIENVGGKIFTFGSYRLGVHTKGADIDALCVAPRHVDRNDFFTSFYDKLKLQEEVKDLRAVEEAFVPVIKLCFDGIEIDILFARLALQTIPEDLDLRDDSLLKNLDIRCIRSLNGCRVTDEILHLV | ||||||
Domain | 220-364 | Poly(A) polymerase central | ||||
Sequence: SFRLTLRAIKLWAKCHNIYSNILGFLGGVSWAMLVARTCQLYPNAIASTLVRKFFLVFSEWEWPNPVLLKEPEERNLNLPVWDPRVNPSDRYHLMPIITPAYPQQNSTYNVSVSTRMVMIEEFKQGLAITHEILLNKAEWSKLFE | ||||||
Domain | 367-435 | Poly(A) polymerase RNA-binding | ||||
Sequence: SFFQKYKHYIVLLASAPTEKQHLEWVGLVESKIRILVGSLEKNEFITLAHVNPQSFPAPKENVDKEEFR | ||||||
Region | 532-552 | Disordered | ||||
Sequence: NSMTAPSPTGTIKTGPLTANP | ||||||
Region | 620-642 | Disordered | ||||
Sequence: LVNHPSRPSGNTATNIPNPILGV |
Sequence similarities
Belongs to the poly(A) polymerase family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length642
- Mass (Da)72,339
- Last updated2004-09-13 v1
- Checksum78E8E2AA201588FC
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 1-19 | Polar residues | ||||
Sequence: MMPFAVTTQGAQQPAPAPK |
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
BC079046 EMBL· GenBank· DDBJ | AAH79046.1 EMBL· GenBank· DDBJ | mRNA | ||
CH474012 EMBL· GenBank· DDBJ | EDL89711.1 EMBL· GenBank· DDBJ | Genomic DNA |