Q68FE2 · ATG9A_MOUSE
- ProteinAutophagy-related protein 9A
- GeneAtg9a
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids839 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Cycles between the preautophagosomal structure/phagophore assembly site (PAS) and the cytoplasmic vesicle pool and supplies membrane for the growing autophagosome (By similarity).
Lipid scramblase activity plays a key role in preautophagosomal structure/phagophore assembly by distributing the phospholipids that arrive through ATG2 (ATG2A or ATG2B) from the cytoplasmic to the luminal leaflet of the bilayer, thereby driving autophagosomal membrane expansion (By similarity).
Also required to supply phosphatidylinositol 4-phosphate to the autophagosome initiation site by recruiting the phosphatidylinositol 4-kinase beta (PI4KB) in a process dependent on ARFIP2, but not ARFIP1 (By similarity).
In addition to autophagy, also plays a role in necrotic cell death (PubMed:27811852).
Catalytic activity
- a 1,2-diacyl-sn-glycero-3-phosphocholine(in) = a 1,2-diacyl-sn-glycero-3-phosphocholine(out)
- a 1,2-diacyl-sn-glycero-3-phospho-L-serine(in) = a 1,2-diacyl-sn-glycero-3-phospho-L-serine(out)
- a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(in) = a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(out)
GO annotations
Keywords
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameAutophagy-related protein 9A
- Alternative names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionQ68FE2
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
Export from the TGN to promote formation of autophagosomes is mediated by the AP-4 complex. Under amino acid starvation or rapamycin treatment, redistributes to preautophagosomal structure/phagophore assembly site (PAS). The starvation-induced redistribution depends on ULK1, ATG13, as well as SH3GLB1. Upon autophagy induction, a portion of transiently localizes to the autophagic membranes (By similarity).
Recruited to damaged mitochondria during mitophagy in a RIMOC1-dependent manner (By similarity).
Features
Showing features for topological domain, transmembrane, intramembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 2-61 | Cytoplasmic | ||||
Sequence: AQFDTEYQRLEASYSDSPPGEEDLLVHVAEGSKSPWHHIENLDLFFSRVYNLHQKNGFTC | ||||||
Transmembrane | 62-84 | Helical | ||||
Sequence: MLIGEMFELMQFLFVVAFTTFLV | ||||||
Topological domain | 85-128 | Lumenal | ||||
Sequence: SCVDYDILFANKMVNHSLHPTEPVKVTLPDAFLPAQVCSARIQE | ||||||
Transmembrane | 129-154 | Helical | ||||
Sequence: NGSLITILVIAGVFWIHRLIKFIYNI | ||||||
Topological domain | 155-290 | Cytoplasmic | ||||
Sequence: CCYWEIHSFYLHALRIPMSALPYCTWQEVQARIVQTQKEHQICIHKRELTELDIYHRILRFQNYMVALVNKSLLPLRFRLPGLGEVVFFTRGLKYNFELILFWGPGSLFLNEWSLKAEYKRGGQRLELAQRLSNRI | ||||||
Intramembrane | 291-301 | |||||
Sequence: LWIGIANFLLC | ||||||
Topological domain | 302-319 | Cytoplasmic | ||||
Sequence: PLILIWQILYAFFSYAEV | ||||||
Intramembrane | 320-328 | |||||
Sequence: LKREPGALG | ||||||
Topological domain | 329-371 | Cytoplasmic | ||||
Sequence: ARCWSLYGRCYLRHFNELEHELQSRLNRGYKPASKYMNCFLSP | ||||||
Transmembrane | 372-397 | Helical | ||||
Sequence: LLTLLAKNGAFFAGSILAVLIALTIY | ||||||
Topological domain | 398-406 | Lumenal | ||||
Sequence: DEDVLAVEH | ||||||
Transmembrane | 407-424 | Helical | ||||
Sequence: VLTTVTLLGVTVTVCRSF | ||||||
Topological domain | 425-470 | Cytoplasmic | ||||
Sequence: IPDQHMVFCPEQLLRVILAHIHYMPDHWQGNAHRSQTRDEFAQLFQ | ||||||
Intramembrane | 471-480 | |||||
Sequence: YKAVFILEEL | ||||||
Topological domain | 481-483 | Cytoplasmic | ||||
Sequence: LSP | ||||||
Intramembrane | 484-492 | |||||
Sequence: IVTPLILIF | ||||||
Topological domain | 493-839 | Cytoplasmic | ||||
Sequence: CLRPRALEIIDFFRNFTVEVVGVGDTCSFAQMDVRQHGHPQWLSGGQTEASVYQQAEDGKTELSLMHFAITNPGWQPPRESTAFLGFLKEQVQRDGAAAGLAQGGLLPENALFTSIQSLQSESEPLSLIANVVAGSSCRGPSLSRDLQGSRHRADVASALRSFSPLQPGAAPQGRVPSTMTGSGVDARTASSGSSVWEGQLQSLVLSEYASTEMSLHALYMHQLHKQQTQAEPERHVWHRRESDESGESAPEEGGEGARAPQPIPRSASYPCATPRPGAPETTALHGGFQRRYGGITDPGTVPRGPSHFSRLPLGGWAEDGQPASRHPEPVPEEGSEDELPPQVHKV |
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
Mice also show aberrant activation of the innate immune response (PubMed:19926846).
Fetal mice display significantly retarded growth (PubMed:26370455).
Conditional deletion in brain causes axon-specific degeneration: mice were born normally, but half of them die within one week, and none live beyond 4 weeks of age (PubMed:28513333).
Defects are caused by impaired autophagy in neurons, leading to progressive degeneration in the axons and their terminals, but not in neuronal cell bodies (PubMed:28513333).
In addition to defects in autophagy, mice also display impaired necrotic cell death during bone morphogenesis: the bone surface is rougher and bones are more porous due to defects in necrotic cell death in bone surface formation (PubMed:27811852).
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 8-11 | Decreased localization to the Golgi apparatus; when associated with 22-A--A-27. | ||||
Sequence: YQRL → AAAA | ||||||
Mutagenesis | 22-27 | Decreased localization to the Golgi apparatus; when associated with 8-A--A-11. | ||||
Sequence: EEDLLV → AAAAA |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 37 variants from UniProt as well as other sources including ClinVar and dbSNP.
Chemistry
PTM/Processing
Features
Showing features for initiator methionine, modified residue, chain, glycosylation.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Initiator methionine | 1 | Removed | ||||
Sequence: M | ||||||
Modified residue | 2 | N-acetylalanine | ||||
Sequence: A | ||||||
Chain | PRO_0000119821 | 2-839 | Autophagy-related protein 9A | |||
Sequence: AQFDTEYQRLEASYSDSPPGEEDLLVHVAEGSKSPWHHIENLDLFFSRVYNLHQKNGFTCMLIGEMFELMQFLFVVAFTTFLVSCVDYDILFANKMVNHSLHPTEPVKVTLPDAFLPAQVCSARIQENGSLITILVIAGVFWIHRLIKFIYNICCYWEIHSFYLHALRIPMSALPYCTWQEVQARIVQTQKEHQICIHKRELTELDIYHRILRFQNYMVALVNKSLLPLRFRLPGLGEVVFFTRGLKYNFELILFWGPGSLFLNEWSLKAEYKRGGQRLELAQRLSNRILWIGIANFLLCPLILIWQILYAFFSYAEVLKREPGALGARCWSLYGRCYLRHFNELEHELQSRLNRGYKPASKYMNCFLSPLLTLLAKNGAFFAGSILAVLIALTIYDEDVLAVEHVLTTVTLLGVTVTVCRSFIPDQHMVFCPEQLLRVILAHIHYMPDHWQGNAHRSQTRDEFAQLFQYKAVFILEELLSPIVTPLILIFCLRPRALEIIDFFRNFTVEVVGVGDTCSFAQMDVRQHGHPQWLSGGQTEASVYQQAEDGKTELSLMHFAITNPGWQPPRESTAFLGFLKEQVQRDGAAAGLAQGGLLPENALFTSIQSLQSESEPLSLIANVVAGSSCRGPSLSRDLQGSRHRADVASALRSFSPLQPGAAPQGRVPSTMTGSGVDARTASSGSSVWEGQLQSLVLSEYASTEMSLHALYMHQLHKQQTQAEPERHVWHRRESDESGESAPEEGGEGARAPQPIPRSASYPCATPRPGAPETTALHGGFQRRYGGITDPGTVPRGPSHFSRLPLGGWAEDGQPASRHPEPVPEEGSEDELPPQVHKV | ||||||
Modified residue | 14 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 16 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 18 | Phosphoserine | ||||
Sequence: S | ||||||
Glycosylation | 99 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Modified residue | 656 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 735 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 738 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 741 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 828 | Phosphoserine | ||||
Sequence: S |
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Gene expression databases
Interaction
Subunit
Interacts (via cytoplasmic its C-terminus) with ATG2A (By similarity).
Interacts with SUPT20H (By similarity).
Interacts (via the tyrosine-based sorting signal motif) with AP4M1; promoting association with the AP-4 complex (By similarity).
Interacts with ARFIP1 and ARFIP2 (By similarity).
Interacts with ATG4A; the interaction is direct and promotes ATG9A trafficking (By similarity).
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for motif, region, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Motif | 8-11 | Tyrosine-based sorting signal | ||||
Sequence: YQRL | ||||||
Region | 657-686 | Disordered | ||||
Sequence: PLQPGAAPQGRVPSTMTGSGVDARTASSGS | ||||||
Compositional bias | 666-686 | Polar residues | ||||
Sequence: GRVPSTMTGSGVDARTASSGS | ||||||
Region | 717-839 | Disordered | ||||
Sequence: HKQQTQAEPERHVWHRRESDESGESAPEEGGEGARAPQPIPRSASYPCATPRPGAPETTALHGGFQRRYGGITDPGTVPRGPSHFSRLPLGGWAEDGQPASRHPEPVPEEGSEDELPPQVHKV | ||||||
Compositional bias | 720-740 | Basic and acidic residues | ||||
Sequence: QTQAEPERHVWHRRESDESGE | ||||||
Compositional bias | 818-839 | Basic and acidic residues | ||||
Sequence: RHPEPVPEEGSEDELPPQVHKV |
Domain
Sequence similarities
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence & Isoform
- Sequence statusComplete
This entry describes 2 isoforms produced by Alternative splicing.
Q68FE2-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- Length839
- Mass (Da)94,425
- Last updated2021-09-29 v2
- Checksum1294E3B9231F1973
Q68FE2-2
- Name2
Computationally mapped potential isoform sequences
There are 4 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A087WP33 | A0A087WP33_MOUSE | Atg9a | 847 | ||
A0A087WQ26 | A0A087WQ26_MOUSE | Atg9a | 176 | ||
A0A087WPL8 | A0A087WPL8_MOUSE | Atg9a | 278 | ||
A0A087WRG3 | A0A087WRG3_MOUSE | Atg9a | 47 |
Features
Showing features for alternative sequence, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Alternative sequence | VSP_061206 | 455-551 | in isoform 2 | |||
Sequence: NAHRSQTRDEFAQLFQYKAVFILEELLSPIVTPLILIFCLRPRALEIIDFFRNFTVEVVGVGDTCSFAQMDVRQHGHPQWLSGGQTEASVYQQAEDG → VHLGGVAESHRHTPHSHLLPPPSGPGDHRLLPQLYGRGRGCGRHLLLCSDGRSPAWPSSVAVWRADRGLSVPASRGREDRVVAHALCHHQSRLAAPS | ||||||
Alternative sequence | VSP_061207 | 552-839 | in isoform 2 | |||
Sequence: Missing | ||||||
Compositional bias | 666-686 | Polar residues | ||||
Sequence: GRVPSTMTGSGVDARTASSGS | ||||||
Compositional bias | 720-740 | Basic and acidic residues | ||||
Sequence: QTQAEPERHVWHRRESDESGE | ||||||
Compositional bias | 818-839 | Basic and acidic residues | ||||
Sequence: RHPEPVPEEGSEDELPPQVHKV |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AM085510 EMBL· GenBank· DDBJ | CAJ30208.1 EMBL· GenBank· DDBJ | mRNA | ||
BC079884 EMBL· GenBank· DDBJ | AAH79884.1 EMBL· GenBank· DDBJ | mRNA | ||
BK004020 EMBL· GenBank· DDBJ | DAA05201.1 EMBL· GenBank· DDBJ | mRNA |