Q68EL2 · DTD2_DANRE

Function

function

Deacylates mischarged D-aminoacyl-tRNAs (By similarity).
Also deacylates mischarged glycyl-tRNA(Ala), protecting cells against glycine mischarging by AlaRS (By similarity).
Probably acts by rejecting L-amino acids from its binding site rather than specific recognition of D-amino acids (By similarity).
Catalyzes the hydrolysis of D-tyrosyl-tRNA(Tyr), has no activity on correctly charged L-tyrosyl-tRNA(Tyr) (By similarity).
By recycling D-aminoacyl-tRNA to D-amino acids and free tRNA molecules, this enzyme counteracts the toxicity associated with the formation of D-aminoacyl-tRNA entities in vivo and helps enforce protein L-homochirality. In contrast to DTD1, deacylates L-Ala mischarged on tRNA(Thr)(G4.U69) by alanine-tRNA ligase AARS (PubMed:29410408).
Can deacylate L-Ala due to a relaxed specificity for substrate chirality caused by the trans conformation of the Gly-Pro motif in the active site (PubMed:29410408).
Also hydrolyzes correctly charged, achiral, glycyl-tRNA(Gly) in vitro, although in vivo eef1a1a/EF-Tu may protect cognate achiral glycyl-tRNA(Gly) from DTD2-mediated deacetylation (By similarity).

Catalytic activity

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular FunctionAla-tRNA(Thr) hydrolase activity
Molecular FunctionD-tyrosyl-tRNA(Tyr) deacylase activity
Molecular FunctiontRNA binding
Biological Processaminoacyl-tRNA metabolism involved in translational fidelity
Biological ProcesstRNA metabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    D-aminoacyl-tRNA deacylase 2
  • EC number
  • Alternative names
    • Animalia-specific tRNA deacylase
      (ATD
      )
    • D-tyrosyl-tRNA deacylase 2
    • L-alanyl-tRNA deacylase

Gene names

    • Name
      dtd2
    • ORF names
      zgc:100795

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Actinopterygii > Neopterygii > Teleostei > Ostariophysi > Cypriniformes > Danionidae > Danioninae > Danio

Accessions

  • Primary accession
    Q68EL2

Proteomes

Organism-specific databases

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00002540511-160D-aminoacyl-tRNA deacylase 2

Proteomic databases

Interaction

Subunit

Homodimer.

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for motif.

TypeIDPosition(s)Description
Motif152-153Gly-transPro motif, allows the protein to recognize chirality of D-amino acids

Domain

A Gly-transPro motif from one monomer fits into the active site of the other monomer to allow specific chiral rejection of most L-amino acids except L-Ala. The trans conformation of the motif is maintained by Arg-143.

Sequence similarities

Belongs to the DTD family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    160
  • Mass (Da)
    17,847
  • Last updated
    2004-10-11 v1
  • Checksum
    EC85396F80C603CD
MKARVVLQQCLHARLQVKPPDEESEAEWVEVNRGMVIYICFFKGATEDLIPKMVNTLLNVKLCETESGKFTSVLQLPGSVLIVPQATLGGKPKGRGMQYHGNIGKDEGLKLYETFVSLCQSELSSCKNSDILTEVKHGTYGNRQVLKLDTNGPYTHLMEF

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
BC080214
EMBL· GenBank· DDBJ
AAH80214.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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