Q67ZB6 · JMJ20_ARATH
- ProteinArginine-specific demethylase JMJ20
- GeneJMJ20
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids462 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Histone demethylase that demethylates 'Arg-3' (H4R3me) of histone H4 with a specific activity for H4R3me2 (PubMed:22483719).
Involved in the positive regulation of gene expression (PubMed:22483719).
Together with JMJ22, positively regulates seed germination by promoting the removal of repressive histone arginine methylations (e.g. H4R3me2) at GA3ox1 and GA3ox2 to trigger gibberellic acid (GA) biosynthesis (PubMed:22483719).
Involved in the positive regulation of gene expression (PubMed:22483719).
Together with JMJ22, positively regulates seed germination by promoting the removal of repressive histone arginine methylations (e.g. H4R3me2) at GA3ox1 and GA3ox2 to trigger gibberellic acid (GA) biosynthesis (PubMed:22483719).
Catalytic activity
- 2-oxoglutarate + N(omega),N(omega)-dimethyl-L-arginyl-[protein] + O2 = CO2 + formaldehyde + N(omega)-methyl-L-arginyl-[protein] + succinateThis reaction proceeds in the forward direction.
Cofactor
Note: Binds 1 Fe2+ ion per subunit.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | nucleus | |
Molecular Function | cis-regulatory region sequence-specific DNA binding | |
Molecular Function | histone H3R2 demethylase activity | |
Molecular Function | histone H4R3 demethylase activity | |
Molecular Function | metal ion binding | |
Biological Process | epigenetic regulation of gene expression | |
Biological Process | gibberellic acid mediated signaling pathway | |
Biological Process | gibberellin mediated signaling pathway | |
Biological Process | positive regulation of seed germination | |
Biological Process | regulation of photomorphogenesis | |
Biological Process | response to red light |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameArginine-specific demethylase JMJ20
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionQ67ZB6
- Secondary accessions
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
Phenotypes & Variants
Disruption phenotype
Plants missing both JMJ20 and JMJ22 exhibit reduced seed germination efficiency during PHYB activation after red light (R)-pulse treatment due to an impaired H4R3me2 removal-dependent derepression of GA3ox1 and GA3ox2 causing lower endogenous gibberellic acid (GA) biosynthesis.
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 46 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000456190 | 1-462 | Arginine-specific demethylase JMJ20 | |||
Sequence: MGIQIIGQIERINGKELSYGDFAERYLAKNQPVVISDLTEDWRAREDWVSENGNPNLHVFATHFGKSRVQVADCDTREFTDQKRLEMSVTEFVEQWTNKDSIEESVLYLKDWHFVKEYPDYTAYQTPPLFSDDWLNVYLDNYQMHEDRDSFQKYDQISCSDYRFVYMGGKGSWTPLHADVFRSYSWSANVCGKKRWLFLPPPQSHLVYDRYMKNCVYDIFEEVNETKFPGFKKTTWLECIQEPGEIIFVPSGWHHQVYNLEDTISINHNWLNAYNLSWVWDLLWKDYKDTEESIEDIRDICDDFEAICQRNLAANTGMNLNDFFLFMSRFSLGNMVLLQSYSDKHKNLNSCSLAMAQNLLMNLSTILKVMMKMISAGGVTAEEVYLDLRETLEDPQFLRFVRDMGRTYARIHMEEEDQFLSSKELLQKLSGLAGPNMQICSPKDLVEMINHHNTFSSQIYFI |
Proteomic databases
Expression
Tissue specificity
Mostly expressed in leaves, and, to a lower extent, in inflorescences, roots, siliques and stems.
Induction
Repressed by the zinc-finger protein SOMNUS when PHYB is inactive in far-red (FR) conditions, but derepressed upon PHYB activation by red light (R).
Developmental stage
In far-red light (FR)-treated seeds, present in the whole embryo (PubMed:22483719).
Accumulates upon red light (R) (PubMed:22483719).
Accumulates upon red light (R) (PubMed:22483719).
Gene expression databases
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 115-287 | JmjC | ||||
Sequence: VKEYPDYTAYQTPPLFSDDWLNVYLDNYQMHEDRDSFQKYDQISCSDYRFVYMGGKGSWTPLHADVFRSYSWSANVCGKKRWLFLPPPQSHLVYDRYMKNCVYDIFEEVNETKFPGFKKTTWLECIQEPGEIIFVPSGWHHQVYNLEDTISINHNWLNAYNLSWVWDLLWKDY |
Sequence similarities
Belongs to the JARID1 histone demethylase family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length462
- Mass (Da)54,358
- Last updated2004-10-11 v1
- ChecksumD6D79E33AE4821B2
Sequence caution
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AB008265 EMBL· GenBank· DDBJ | BAB10550.1 EMBL· GenBank· DDBJ | Genomic DNA | Sequence problems. | |
CP002688 EMBL· GenBank· DDBJ | AED97697.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AK175825 EMBL· GenBank· DDBJ | BAD43588.1 EMBL· GenBank· DDBJ | mRNA | ||
AK176202 EMBL· GenBank· DDBJ | BAD43965.1 EMBL· GenBank· DDBJ | mRNA | ||
BT023460 EMBL· GenBank· DDBJ | AAY56451.1 EMBL· GenBank· DDBJ | mRNA |